Cell-cell interaction by mouse limb cells during in vitro chondrogenesis: Analysis of the brachypod mutation

Owens, Elizabeth M.; Solursh, Michael

doi:10.1016/0012-1606(82)90043-4

Cited by 41 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together the data of the expression pattern, of the brachypodism mice (Owens and Solursh, 1982) and our in vitro data it is most likely that the primary physiological role of GDF5 is from the early stage of chondrogenesis and chondrocyte differentiation. It is interesting to hypothesize that GDF5 may act mainly on mesenchymal progenitor cells, directing their differentiation into cartilage without directly converting the progenitor cells into osteoblasts during the process of endochondral bone formation in the skeletal development of limbs.…”

Section: Discussionmentioning

confidence: 55%

“…One of the BMP related genes, called GDF5, may be required for the normal skeletal development of limbs as suggested by mutations of the GDF5 gene in brachypodism mice (Storm et al, 1994). The in vitro analyses of the mutant mice have suggested that the production of a signal stimulating mesenchyme aggregation and chondrogenesis in limbs may be disrupted (Owens and Solursh, 1982;Malinina et al, 1984). More recently, the study of cartilage-derived morphogenetic proteins (CDMPs) has shown that CDMP-1 which was proposed to be the GDF5 equivalent, is predominantly expressed at the stage of precartilaginous mesenchymal condensation and throughout the cartilaginous cores of the developing long bones (Chang et al, 1994).…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Recombinant Human Growth/Differentiation Factor 5 Stimulates Mesenchyme Aggregation and Chondrogenesis Responsible for the Skeletal Development of Limbs

Hötten

Matsumoto²,

Kimura³

et al. 1996

Growth Factors

149

View full text Add to dashboard Cite

We have expressed and biologically characterized recombinant human growth/differentiation factor 5 (huGDF5). This protein is composed of a mature homodimer consisting of 15 kD subunits. Using recombinant expressed protein, we have demonstrated that huGDF5 in vitro stimulated mesenchyme aggregation and chondrogenesis in rat limb bud cells. In vivo, partially purified huGDF5 induced cartilage and bone formation in muscular tissues of rodents. However, in contrast to the effects of other BMPs, as for example BMP-2, the osteoblastic MC3T3-E1 cells did not respond to huGDF5 as measured by alkaline phosphatase activity. These results suggest that the action of GDF5 may be relatively specific for chondrogenesis during the entire process of the endochondral bone formation. GDF5 may control the morphogenesis of cartilaginous tissue, including joints, in the skeletal development of limbs.

show abstract

Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 95%

Recombinant Human Growth/Differentiation Factor 5 Stimulates Mesenchyme Aggregation and Chondrogenesis Responsible for the Skeletal Development of Limbs

Hötten

Matsumoto²,

Kimura³

et al. 1996

Growth Factors

149

View full text Add to dashboard Cite

show abstract

“…Screening of the literature revealed that the bp mutation is a single gene mutation affecting several steps in early limb development resulting in a distinct shortening of the limbs. Careful analysis of the underlying cellular defect demonstrated that the primary defect in bp mice is due to the reduced ability of a specific mesenchymal cell population to provide an inductive chondrogenic stimulus (Owens and Solursh 1982). These data strongly suggest that a mutation in CDMP-1 was responsible for the bp phenotype in mice.…”

Section: Role Of the Cdmps In Skeletal Deveioprnentmentioning

confidence: 96%

Cartilage-derived morphogenetic proteins Key regulators in chondrocyte differentiation?

Luyten

1995

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

“…Brachydactyly and postaxial hemimelia characterize the mutant phenotype, first recognized as abnormal on day 13.5 of gestation (Grfineberg and Lee 1973), and have been attributed to a local defect of homotypic mesenchymal interactions Offprint requests to: W.A. Etmer in the limb (Owens and Solursh 1982). Affected regions exhibit altered temporal differentiation of precartilage anlagen that are reduced in size.…”

Section: Introductionmentioning

confidence: 99%

Morphological analysis of abnormal digital chondrogenesis in the Brachypod (bpH) mouse limb in organ culture

et al. 1992

View full text Add to dashboard Cite

Brachypod (bpH/bpH), an autosomal mutation in mice, is characterized by a shortening of the long bones and paws, and a delay or absence of ossification in some of the distal limb elements. The present study represents a detailed description of the brachypod phenotype in day 12 hindlimb buds maintained for 6 days in a submerged, serum-free organ culture system. Using this in vitro system, the proximal-to-distal effect on the severity of cartilage reduction was intensified in the brachypod explants with an intermediate expression in the heterozygotes. Immunofluorescent staining of the brachypod cartilage revealed a deficiency in and an abnormal distribution of the proteoglycans. Although there was no recognizable difference in the immunofluorescent staining for type II collagen between the mutant and wild-type, electron micrographs showed the presence of thick fibrils in the matrix. Other atypical structures in the brachypod cartilage included pleomorphic nuclei, reduced intracellular glycogen granules and profuse intercellular contacts. It is proposed that with the use of this in vitro system which supports the autonomous development of the individual limb elements, experiments concerning the pathogenesis of skeletal mutations such as brachypod should be more feasible.

show abstract

Cell-cell interaction by mouse limb cells during in vitro chondrogenesis: Analysis of the brachypod mutation

Cited by 41 publications

References 15 publications

Recombinant Human Growth/Differentiation Factor 5 Stimulates Mesenchyme Aggregation and Chondrogenesis Responsible for the Skeletal Development of Limbs

Recombinant Human Growth/Differentiation Factor 5 Stimulates Mesenchyme Aggregation and Chondrogenesis Responsible for the Skeletal Development of Limbs

Cartilage-derived morphogenetic proteins Key regulators in chondrocyte differentiation?

Morphological analysis of abnormal digital chondrogenesis in the Brachypod (bpH) mouse limb in organ culture

Contact Info

Product

Resources

About