Cell competition: how to eliminate your neighbours

Amoyel, Marc; Bach, Erika A.

doi:10.1242/dev.079129

Cited by 189 publications

(173 citation statements)

References 129 publications

(272 reference statements)

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“…6B). On the other hand, we cannot exclude the possibility that the higher apoptosis rate could be a result of increased cell competition in the tumor brain (29). The lower apoptosis rate in the tumor brain at 48-96 h ALH might suggest that the tumor cells have evaded apoptosis.…”

Section: Infrared Fluorescent Executioner-caspase Reporter Visualizesmentioning

confidence: 90%

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Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

Piggott

Makhijani

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

119

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Fluorescence resonance energy transfer-based reporters have been widely used in imaging cell signaling; however, their in vivo application has been handicapped because of poor signal. Although fluorogenic reporters overcome this problem, no such reporter of proteases has been demonstrated for in vivo imaging. Now we have redesigned an infrared fluorescent protein so that its chromophore incorporation is regulated by protease activity. Upon protease activation, the infrared fluorogenic protease reporter becomes fluorescent with no requirement of exogenous cofactor. To demonstrate biological applications, we have designed an infrared fluorogenic executioner-caspase reporter, which reveals spatiotemporal coordination between cell apoptosis and embryonic morphogenesis, as well as dynamics of apoptosis during tumorigenesis in Drosophila. The designed scaffold may be used to engineer reporters of other proteases with specific cleavage sequence.

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Section: Infrared Fluorescent Executioner-caspase Reporter Visualizesmentioning

confidence: 90%

“…The lower apoptosis rate in the tumor brain at 48-96 h ALH might suggest that the tumor cells have evaded apoptosis. Alternatively, the lower apoptosis rate could suggest that "vulnerable" cells may have died off because of cell competition (29).…”

Section: Infrared Fluorescent Executioner-caspase Reporter Visualizesmentioning

confidence: 99%

Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

Piggott

Makhijani

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

119

View full text Add to dashboard Cite

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“…These results imply that at the early stage of carcinogenesis, normal epithelial cells act as 'immunity' against transformed cells, and we have named this process 'epithelial defense against cancer' (EDAC) . In Drosophila, it has been also shown that normal and transformed epithelial cells often compete with each other for survival, and this phenomenon is called cell competition (Amoyel and Bach, 2014;Baker, 2011;Moreno and Rhiner, 2014). However, detailed molecular mechanisms regulating these non-cell-autonomous processes still remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

EPLIN is a crucial regulator for extrusion of RasV12-transformed cells

Ohoka

Kajita

Ikenouchi

et al. 2015

Journal of Cell Science

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At the initial stage of carcinogenesis, a mutation occurs in a single cell within a normal epithelial layer. We have previously shown that RasV12-transformed cells are apically extruded from the epithelium when surrounded by normal cells. However, the molecular mechanisms underlying this phenomenon remain elusive. Here, we demonstrate that Cav-1-containing microdomains and EPLIN (also known as LIMA1) are accumulated in RasV12-transformed cells that are surrounded by normal cells. We also show that knockdown of Cav-1 or EPLIN suppresses apical extrusion of RasV12-transformed cells, suggesting their positive role in the elimination of transformed cells from epithelia. EPLIN functions upstream of Cav-1 and affects its enrichment in RasV12-transformed cells that are surrounded by normal cells. Furthermore, EPLIN regulates non-cell-autonomous activation of myosin-II and protein kinase A (PKA) in RasV12-transformed cells. In addition, EPLIN substantially affects the accumulation of filamin A, a vital player in epithelial defense against cancer (EDAC), in the neighboring normal cells, and vice versa. These results indicate that EPLIN is a crucial regulator of the interaction between normal and transformed epithelial cells.

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“…Among the various types of communication, cell competition is a unique type of short-range communication originally identified in Drosophila. Cells compare their fitness level with those of neighboring cells, and cells with relatively higher fitness become winners, and cells with relatively lower fitness are eliminated as losers (for reviews, see Baker, 2011;Levayer and Moreno, 2013;Vincent et al, 2013;Amoyel and Bach, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cell competition in mouse NIH3T3 embryonic fibroblasts controlled by Tead activity and Myc

Mamada

Satō

Ota

et al. 2015

Journal of Cell Science

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Cell competition is a short-range communication originally observed in Drosophila. Relatively little is known about cell competition in mammals or in non-epithelial cells. Hippo signaling and its downstream transcription factors of the Tead family, control cell proliferation and apoptosis. Here, we established an in vitro model system that shows cell competition in mouse NIH3T3 embryo fibroblast cells. Co-culture of Tead-activity-manipulated cells with normal (wild-type) cells caused cell competition. Cells with reduced Tead activity became losers, whereas cells with increased Tead activity became super-competitors. Tead directly regulated Myc RNA expression, and cells with increased Myc expression also became super-competitors. At low cell density, cell proliferation required both Tead activity and Myc. At high cell density, however, reduction of either Tead activity or Myc was compensated for by an increase in the other, and this increase was sufficient to confer 'winner' activity. Collectively, NIH3T3 cells have cell competition mechanisms similar to those regulated by Yki and Myc in Drosophila. Establishment of this in vitro model system should be useful for analyses of the mechanisms of cell competition in mammals and in fibroblasts.

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Cell competition: how to eliminate your neighbours

Cited by 189 publications

References 129 publications

Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

EPLIN is a crucial regulator for extrusion of RasV12-transformed cells

Cell competition in mouse NIH3T3 embryonic fibroblasts controlled by Tead activity and Myc

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