Cell Culture Chip Using Low-Shear Mass Transport

Liu, Ke; Pitchimani, Rajasekar; Dang, Dana; Bayer, Keith; Harrington, Tyler; Pappas, Dimitri

doi:10.1021/la8003917

Cited by 52 publications

(44 citation statements)

References 22 publications

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“…Using high flow rates in the primary channel, the microvortex generated by flow detachment in the side chamber enabled the investigation of high radial acceleration and shear stress applied to single cells. These relatively high shear stress levels are of interest because they may induce cellular stress responses [18]. For multiple perspective microscopy, low flow rates and low shear stress in the microvortex provide a more favorable environment including low shear stress on the rotated cell [10].…”

Section: Existing Techniques For Single Cell Rotationmentioning

confidence: 99%

Simulation and Experimental Characterization of Microscopically Accessible Hydrodynamic Microvortices

et al. 2012

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Abstract:Single-cell studies of phenotypic heterogeneity reveal more information about pathogenic processes than conventional bulk-cell analysis methods. By enabling high-resolution structural and functional imaging, a single-cell three-dimensional (3D) imaging system can be used to study basic biological processes and to diagnose diseases such as cancer at an early stage. One mechanism that such systems apply to accomplish 3D imaging is rotation of a single cell about a fixed axis. However, many cell rotation mechanisms require intricate and tedious microfabrication, or fail to provide a suitable environment for living cells. To address these and related challenges, we applied numerical simulation methods to design new microfluidic chambers capable of generating fluidic microvortices to rotate suspended cells. We then compared several microfluidic chip designs experimentally in terms of: (1) their ability to rotate biological cells in a stable and precise manner; and (2) their suitability, from a geometric standpoint, for microscopic cell imaging. We selected a design that incorporates a trapezoidal side chamber connected to a main flow channel because it provided well-controlled circulation and met imaging requirements. Micro particle-image velocimetry (micro-PIV) was used to provide a detailed characterization of flows in the new design. Simulated and experimental results OPEN ACCESSMicromachines 2012, 3 530 demonstrate that a trapezoidal side chamber represents a viable option for accomplishing controlled single cell rotation. Further, agreement between experimental and simulated results confirms that numerical simulation is an effective method for chamber design.

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Section: Existing Techniques For Single Cell Rotationmentioning

confidence: 99%

Simulation and Experimental Characterization of Microscopically Accessible Hydrodynamic Microvortices

et al. 2012

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“…A simple cell culture chip suitable for suspension cells was presented by Liu et al, (2008). They have fabricated a microfluidic device with minimum shear stress.…”

Section: Sample Preparation: Expansion Arrest and Fixation Of Chromomentioning

confidence: 99%

Microtechnologies Enable Cytogenetics

Kwasny¹,

Vedarethinam²,

Shah³

et al. 2012

Recent Trends in Cytogenetic Studies - Methodologies and Applications

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“…Studies have shown good viability of living cells when grown on cellular microenvironmental chips [359][360][361][362][363][364][365][366][367][368][369][370]. Microfluidics replaces the parallel plate chambers for cell growth, and shear stress investigations establish the need to match the length scales between the fluidic channels and the cells [359][360][361][362][363][364][365][366][367][368][369][370].…”

Section: Biocompatibility and Cell Viability Within Microfluidic Systemsmentioning

confidence: 99%

“…Microfluidics replaces the parallel plate chambers for cell growth, and shear stress investigations establish the need to match the length scales between the fluidic channels and the cells [359][360][361][362][363][364][365][366][367][368][369][370].…”

Section: Biocompatibility and Cell Viability Within Microfluidic Systemsmentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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This paper reviews microfluidic technologies with emphasis on applications in the fields of pharmacy, biology, and tissue engineering. Design and fabrication of microfluidic systems are discussed with respect to specific biological concerns, such as biocompatibility and cell viability. Recent applications and developments on genetic analysis, cell culture, cell manipulation, biosensors, pathogen detection systems, diagnostic devices, high-throughput screening and biomaterial synthesis for tissue engineering are presented. The pros and cons of materials like polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polystyrene (PS), polycarbonate (PC), cyclic olefin copolymer (COC), glass, and silicon are discussed in terms of biocompatibility and fabrication aspects. Microfluidic devices are widely used in life sciences. Here, commercialization and research trends of microfluidics as new, easy to use, and cost-effective measurement tools at the cell/tissue level are critically reviewed.

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Cell Culture Chip Using Low-Shear Mass Transport

Cited by 52 publications

References 22 publications

Simulation and Experimental Characterization of Microscopically Accessible Hydrodynamic Microvortices

Simulation and Experimental Characterization of Microscopically Accessible Hydrodynamic Microvortices

Microtechnologies Enable Cytogenetics

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

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