Cell culture from tissues of grass prawn, Penaeus monodon.

Chen, Shiu-Nan; Chi, Shau-Chi; Kou, Guang Hsiung; Liao, I-Chiu

doi:10.3147/jsfp.21.161

Cited by 87 publications

(62 citation statements)

References 7 publications

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“…The earliest attempts on shrimp cell culture development appeared as published document in 1986 by Chen and colleagues from National Taiwan University, Taiwan [9]. They had chosen Penaeus monodon as the species of choice from which several cell culture systems could be generated using various tissues and organs.…”

Section: History Of Shrimp Cell Culturementioning

confidence: 99%

“…Considering the importance of inorganic salts for the maintenance of ionic balance and osmotic pressure [61], researchers have used KCl, MgSO 4 , MgCl 2 , and CaCl 2 at concentrations ranging from 0.9 to 3 g/l to supplement the required quantity in the growth medium [39,48,49]. To adjust osmolality, NaCl at a concentration ranging from 6 to 12 g/l [9,19,40,49] has also been added besides the balanced salt solutions [40,78]. Addition of vitamins [41,42], proline [49,56,57,82] and glutamine [28,82] has been proven to be the choice of supplements in the growth media.…”

Section: Organic and Inorganic Supplements To Improve Growth Of Shrimmentioning

confidence: 99%

“…Since the first attempt on shrimp cell line development, performed in 1986 by Chen et al [9], P. monodon remained the best sought after candidate species among all penaeids in the development of cell cultures; may be due to its availability in all South East Asian Countries and its popularity as the most widely cultured species. Of the 50 selected publications, 17 reported (34 %) P. monodon [2-4, 7, 8, 10, 15, 23, 35, 41, 42, 44, 58, 69, 83, 89, 90] as the species of choice, eight researchers (16 %) used Penaeus japonicus [10,39,47,48,[55][56][57]72], seven (17 %) selected Penaeus chinensis [13, 19, 36-38, 40, 80] and Penaeus vannamei [18,26,27,49,51,63,82].…”

Section: Species Of Choice: a Major Concernmentioning

confidence: 99%

“…[27,46,62,80]. Besides, testis [62,81], heart [9,10,48,62,80], hepatopancreas [9,28,48,55,58,62,64,81,89], gill [9,62], nerve [9,13,48,62,80,81], muscle [9,27,48], hematopoietic tissue [11,62,90], embryonic tissue [19,80,82], epidermis [81,82], gut [9,62] and Y organ [81] were also widely used for cell culture development (Fig. 3).…”

Section: Tissues and Organs Used For Cell Culture Developmentmentioning

confidence: 99%

“…However, it still remains unattained presenting researchers more questions than answers [9,65]. Till date, no permanent cell line could be made available from marine invertebrates in general [67] and shrimp in particular.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of Shrimp Cell Lines: Perception and Orientation

2012

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Development of continuous shrimp cell lines for effective investigation on shrimp viruses remains elusive with an arduous history of over 25 years. Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. Though improved growth and longevity of shrimp cells in vitro could be achieved by using modified growth media this did not make any leap to spontaneous transformation; probably due to the fact that shrimp cells inhibited neoplastic transformations. Oncogenic induction and immortalization are considered as the possible ways, and an exclusive medium for shrimp cell culture and an appropriate mode of transformation are crucial. In this review status of shrimp cell line development and its future orientation are discussed.

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Section: History Of Shrimp Cell Culturementioning

confidence: 99%

Section: Organic and Inorganic Supplements To Improve Growth Of Shrimmentioning

confidence: 99%

Section: Species Of Choice: a Major Concernmentioning

confidence: 99%

Section: Tissues and Organs Used For Cell Culture Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment of Shrimp Cell Lines: Perception and Orientation

2012

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Yolk protein synthesis in the riceland tadpole shrimp,Triops longicaudatus, measured by in vitro incorporation of3H-leucine

Riley

Tsukimura

1998

J. Exp. Zool.

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Investigation of reproductive control within the tadpole shrimp, Triops longicaudatus, required the isolation and characterization of the yolk protein (vitellin, Vn). To this end, tadpole shrimp were cultured in environmental chambers (29°C–22°C, with 14:10 light:dark cycle). Desiccated cysts hatched in 2–3 days after inundation. The tadpole shrimp began egg deposition 7 to 8 days after hatching and exhibited a mean growth rate of 1.85 ± 0.24 mm/day. It was observed that 4‐day‐old shrimp had visible eggs in their ovaries. In addition, Vn was isolated and characterized from reproductive animals, resolving as one protein on native PAGE, and possessing a molecular weight (MW) of 376,000 ± 2,900 as determined by FPLC. Examination by SDS‐PAGE revealed that Vn is composed of a single molecule with a MW of 214,000 ± 2,000. Methyl farnesoate (MF), a crustacean compound whose role in reproduction is still being elucidated and is structurally similar to juvenile hormone III (JH III) was incubated with ovarian explants. These explants were incubated for 24 h at room temperature in EAGLE's medium adjusted to Van Harreveld's solution in six concentrations (1 pM to 100 nM) of MF and JH III. Methyl farnesoate and JH III had no direct in vitro effect on yolk protein synthesis (P ≤ 0.545 and P ≤ 0.815, respectively). J. Exp. Zool. 281:238–247, 1998. © 1998 Wiley‐Liss, Inc.

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Antigenic characterization ofNephrops norvegicus (L.) hepatopancreas cells

Lyons-Alcantara¹,

Lambkin²,

Mothersill³

1999

Cell Biochem. Funct.

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During this work structural, differentiation and proliferation antigenic markers developed for mammals were applied in paraffin sections of Nephrops norvegicus (L.) hepatopancreas. The purpose was to establish standards for the characterization of invertebrate cells in vitro. Antibody concentration was optimized for quantification of cell proliferation. There are no antibodies specific for crustaceans on the market. An avidin-biotin immunoperoxidase method was used to visualize cell antigen expression. The immunocytochemical results indicate that the epithelium in the Nephrops hepatopancreas digestive tubules does express cytokeratins and proliferating cell nuclear antigen. The results of this work indicate that some mammalian antibodies cross-react with crustacean epitopes. This may facilitate cell characterization of cell types cultured in vitro.

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Cell culture from tissues of grass prawn, Penaeus monodon.

Cited by 87 publications

References 7 publications

Establishment of Shrimp Cell Lines: Perception and Orientation

Establishment of Shrimp Cell Lines: Perception and Orientation

Yolk protein synthesis in the riceland tadpole shrimp,Triops longicaudatus, measured by in vitro incorporation of3H-leucine

Antigenic characterization ofNephrops norvegicus (L.) hepatopancreas cells

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