Cell Culture Imaging Using Microimpedance Tomography

Linderholm, Pontus; Marescot, L.; Loke, M.H.; Renaud, Philippe

doi:10.1109/tbme.2007.910649

Cited by 66 publications

(28 citation statements)

References 34 publications

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“…Microelectrode systems have been successfully used to investigate growth and migration of monolayer cell cultures. By applying a voltage across a sample and then measuring the current flow using different kinds of measurement method, various cell functions have been monitored [3][4][5]. Methods such as scanning impedance imaging have also been applied to cell-culture studies [6,7].…”

Section: Introductionmentioning

confidence: 99%

Development of a prototype micro-EIT system using three sets of 15×8 array electrodes

Liu

et al. 2010

J. Phys.: Conf. Ser.

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We developed a new microscopic electrical impedance tomography (micro-EIT) system to visualize admittivity distributions within a miniature hexahedral container, where we place small biological samples with a background solution or gel. Each of two facing sides (left and right) of the container is fully covered by a solid metal electrode. We inject current between them, thereby producing a uniform parallel current flow along the longitudinal direction inside the container. Each of three sides at the bottom, front and back is equipped with a 15×8 array of voltage-sensing electrodes. Switching modules are located underneath the container so that we can measure voltage between any neighbouring pair of electrodes. Three switching modules are connected to a 16-channel multi-frequency EIT system to collect induced voltage data from the three sets of 15×8 array electrodes subject to the single fixed current injection. Voltage data set from 360 voltage-sensing electrodes on three sides are utilized to produce cross-sectional images of the admittivity distribution. We describe the design and construction of the new micro-EIT system. Our future work should include development of a customized image reconstruction algorithm for the micro-EIT system and experimental validation.

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Section: Introductionmentioning

confidence: 99%

Development of a prototype micro-EIT system using three sets of 15×8 array electrodes

Liu

et al. 2010

J. Phys.: Conf. Ser.

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“…Indeed, electrical resistivity tomography method is a non-invasive technique that enables to obtain a large number of resistivity data in a short period of time which has been widely used in environmental and engineering [15][16][17][18][19], hydrological [20,21], archaeological [22,23] and mineral exploration [24][25][26] surveys. In addition, it has been used to image structures from the millimetre scale to kilometres [27,28].…”

Section: Introductionmentioning

confidence: 99%

Electrical Resistivity Tomography as a Support Tool to Estimate Physicochemical Properties of Mining Tailings Pond

Gabarrón¹,

Martínez‐Pagán²,

Martínez-Segura³

et al. 2020

Preprint

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Legacy mining industry has left a large number of tailings ponds exposed to water and wind erosion that causes serious environmental and health problems. Prior to rehabilitation actions a deep sampling of the materials infilling the pond used to be necessary. Thus, the primary objective of this study is to demonstrate the usefulness of the Electrical Resistivity Tomography (ERT) method as a non-invasive tool to determine the physicochemical composition of mine tailings ponds, enabling more efficient and low-cost surveys. To achieve this objective, three ERT profiles and three boreholes in each profile were carried out, from each borehole three waste samples from differents depths were collected and a geochemical characterization of the samples was carried. In order to estimate the composition of the infilling wastes in tailing ponds from electrical resistivity measures, several regression models were calculated for different physicochemical properties and metal concentrations. As a result, a high resistivity area was depicted in profiles G2 and G3 while a non-resistive area (profile G1) was also found. Relationships among low resistivity values and high salinity, clay content and high metal concentrations and mobility were established. Specifically, calibrated models were obtained for electrical conductivity, particles sizes of 0.02-50 µm and 50-2000 µm, total Zn and Cd concentration, and bioavailable Ni, Cd and Fe. Therefore, the ERT technique could be considered as a useful tool for mine tailings ponds characterization, and it can be used to estmate some physicochemical properties and metal concentrations of this mine waste.

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“…Electrical Impedance Tomography (EIT) [1] is being researched in different areas of science and technology due to its many advantages [2][3][4] over other computed tomographic techniques [5]. Being a non-invasive, nonradiating, non-ionizing and inexpensive methodology, EIT has been extensively studied in clinical diagnosis [6], biomedical engineering [7] and biotechnology [8]. Attempts are also being made to develop a better medical-EIT system (Fig.-1) for different clinical investigations [9][10][11][12][13][14][15][16][17][18] and long time patient monitoring.…”

Section: Introductionmentioning

confidence: 99%

Improving Image Quality in Electrical Impedance Tomography (EIT) Using Projection Error Propagation-Based Regularization (PEPR) Technique: A Simulation Study

Bera

Biswas

Rajan

et al. 2011

Journal of Electrical Bioimpedance

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A Projection Error Propagation-based Regularization (PEPR) method is proposed and the reconstructed image quality is improved in Electrical Impedance Tomography (EIT). A projection error is produced due to the misfit of the calculated and measured data in the reconstruction process. The variation of the projection error is integrated with response matrix in each iterations and the reconstruction is carried out in EIDORS. The PEPR method is studied with the simulated boundary data for different inhomogeneity geometries. Simulated results demonstrate that the PEPR technique improves image reconstruction precision in EIDORS and hence it can be successfully implemented to increase the reconstruction accuracy in EIT.><p><a href="http://dx.doi.org/10.5617/jeb.158" target="_blank">doi:10.5617/jeb.158 </a><br />J Electr Bioimp, vol. 2, pp. 2-12, 2011</p>

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Cell Culture Imaging Using Microimpedance Tomography

Cited by 66 publications

References 34 publications

Development of a prototype micro-EIT system using three sets of 15×8 array electrodes

Development of a prototype micro-EIT system using three sets of 15×8 array electrodes

Electrical Resistivity Tomography as a Support Tool to Estimate Physicochemical Properties of Mining Tailings Pond

Improving Image Quality in Electrical Impedance Tomography (EIT) Using Projection Error Propagation-Based Regularization (PEPR) Technique: A Simulation Study

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