Cell cultures from bone affected by pacet's disease

Mills, Barbara G.; Singer, Frederick R.; Weiner, Leslie P.; Holst, Patricia

doi:10.1002/art.1780231007

Cited by 45 publications

(11 citation statements)

References 13 publications

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“…Doubling times were similar to those found by others using this technique: 5 days [6] and 4-12 days [10,11]. The mean doubling times among the three groups of subjects were very similar.…”

Section: Discussionsupporting

confidence: 87%

“…This technique has not been widely used in the study of metabolic bone disease. However, over the past several years it has been used to study patients with Paget's disease [10], diseases of skeletal development [11], renal osteodystrophy [12] and pseudohypoparathyroidism [13,14]. Recently we [1] and others [15][16][17][18] have applied the explant technique to derive osteobtast-like cells from osteoporotic patients.…”

Section: Introductionmentioning

confidence: 99%

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In vitro study of osteoblastic cells from patients with idiopathic osteoporosis and comparison with cells from non-osteoporotic controls [1]

Wong

Rao

et al. 1994

Osteoporosis Int

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We have examined bone cells derived from iliac crest trabecular explants of 30 patients with idiopathic osteoporosis and 45 control subjects in order to determine whether intrinsic abnormalities in osteoblast function may contribute to the decreased bone formation observed in this disease. Bone cells isolated from all subjects expressed several in vitro characteristics of the osteoblast phenotype including adenylate cyclase responsiveness to parathyroid hormone (PTH) and prostaglandin E1 (PGE1), basal and 1,25(OH)2D3-stimulated alkaline phosphatase activity and osteocalcin production. Results were compared amongst three subject groups; young controls less than 40 years old, older controls over 40 years old, and osteoporotics. Osteoporotic cells were found in general to be fully active in vitro. There were no differences between osteoporotic and control cells in their basal levels of adenylate cyclase, or alkaline phosphatase, in their growth rates, or cell morphology. The cyclic AMP (cAMP) response to PTH was significantly lower in osteoporotic cells (71%, p < 0.01) and older control cells (64%, p < 0.005) relative to the response in cells from younger controls, suggesting that the decreased responsiveness in osteoporotic cells was due to subject age rather than the osteoporotic state. At the same time, the cAMP responses to PGE1 and cholera toxin were similar in cells from all three subject groups. The response to forskolin was reduced to about 40% in osteoporotic cells compared with controls, but this was not mirrored by similar differences in the responses to PTH, PGE1 or cholera toxin, suggesting that the availability of catalytic subunits is not rate-limiting in these cells. 1,25(OH)2D3-stimulated osteocalcin production was 220% higher in osteoporotics than in older controls, but the numbers tested were small and the difference did not reach significance. The one significant abnormality we observed in osteoporotic cells was in alkaline phosphatase activity: 1,25(OH)2D3-stimulated alkaline phosphatase activity was twofold higher in osteoporotics than in younger (p < 0.05), older (p < 0.05) and pooled controls (p < 0.025). The significance of this finding is unknown, but we postulate that it may reflect an intrinsic abnormality in osteoblast function in patients with idiopathic osteoporosis.

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“…Doubling times were similar to those found by others using this technique: 5 days [6] and 4-12 days [10,11]. The mean doubling times among the three groups of subjects were very similar.…”

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

In vitro study of osteoblastic cells from patients with idiopathic osteoporosis and comparison with cells from non-osteoporotic controls [1]

Wong

Rao

et al. 1994

Osteoporosis Int

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show abstract

“…However, these patients do not have high titers of circulating measles antibodies when compared with control subjects [49]. In the same disease, respiratory syncytial virus antigens were reported in cultured bone cells [36]. As for inclusion-body myositis, further studies are needed to sustain these viral hypotheses.…”

Section: Discussionmentioning

confidence: 99%

Inclusion‐body myositis: Clinicopathological studies and isolation of an adenovirus type 2 from muscle biopsy specimen

Mikol

Felten‐Papaiconomou

Ferchal

et al. 1982

Annals of Neurology

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A case of inclusion‐body myositis appearing clinically as an atypical distal atrophy lasting for more than 14 years was diagnosed by visualization of characteristic filamentous inclusions in subsarcolemmal nuclei and in the sarcoplasm near vacuoles. A strain of adenovirus type 2 was isolated from two successive muscle biopsy specimens, and the serum neutralizing antibody titer to this strain was 1:64. The data provide objective support for the assumption that inclusion‐body myositis is caused by a virus infection of muscle, although the filamentous inclusions differ from all known viral structures.

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“…Rebel et al (1980) have demonstrated such antigens from measles virus using both immunofluorescence and immunoperoxidase (Heyderman 1979) techniques. Using similar methodology, Mills et al (1980) have demonstrated similar antigens from RSV and, more recently, elements of both viruses (Mills et al 1984) ! Basle et al (1983) have also identified 4 of 6 structural measles antigens in Pagetic osteoclasts, using a monoclonal antibody technique, and emphasized the absence of two antigens associated with viral replication; the significance of this is discussed later.…”

Section: Introductionmentioning

confidence: 95%

Viral Aetiology of Paget's Disease of Bone: A Review

Harvey

1984

J R Soc Med

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Cell cultures from bone affected by pacet's disease

Cited by 45 publications

References 13 publications

In vitro study of osteoblastic cells from patients with idiopathic osteoporosis and comparison with cells from non-osteoporotic controls [1]

In vitro study of osteoblastic cells from patients with idiopathic osteoporosis and comparison with cells from non-osteoporotic controls [1]

Inclusion‐body myositis: Clinicopathological studies and isolation of an adenovirus type 2 from muscle biopsy specimen

Viral Aetiology of Paget's Disease of Bone: A Review

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