Cell cultures of neuroblasts from rat olfactory epithelium that show odorant responses.

Abstract

We have developed procedures that permit isolation and propagation of clonal cell cultures from the olfactory epithelium of the 5-to 7-day-old rat that appear to represent the neuroblasts that repopulate the sensory neurons in the olfactory epithelium throughout life. The cell lines we report here synthesize neuron-specific enolase, which is a neuron marker, 43-kDa growth-associaited protein, a protein associated with neuronal growth cones, and carnosine, a possible olfactory neurotransmitter. In two of the cell lines we have found dose-dependent cAMP accumulation following exposure to submicromolar concentrations of chemical odorants in the medium. These two cell lines show different patterns of odorant specificity when tested against a panel of six chemicals commonly used as test odorants. We anticipate that these and similarly derived cell lines will prove valuable in studying aspects of neurogenesis and olfaction.

Secretion of Protease Nexin-1 by C6 Glioma Cells Is under the Control of a Heterotrimeric G Protein, Go1

“…Furthermore, GAP43-(1-10), a permeant peptide derived from the amino-terminal 10 residues of GAP43, was also able to stimulate the secretion of PN-1 in a non-additive manner with mastoparan. GAP43 and APP are expressed in C6 glioma cells (57,58) and could be potential endogeneous activators of G o1 ␣ as previously reported (6,7). It is also possible that some G protein-coupled receptors might accelerate the GDP-GTP exchange on G o1 ␣ at the vesicle membrane in order to control final steps in the constitutive secretory pathway.…”

Olfactory mucosa for transplant‐mediated repair: A complex tissue for a complex injury?

“…The neuronal cells from human fetal olfactory tissue were found to have a typical bipolar morphology and expressed specific markers for ORNs, as previously described in primary cultures of ORNs in the rat (Coon et al, 1989;Pixley et al, 1990;Ronnett et al, 1991). These cells harvested from adult human cadavers expressed both neuronal and epithelial markers and exhibited odorant-dependent cAMP and calcium accumulation (Wolozin et al, 1992).…”

Long-term culture and functional characterization of follicular cells from adult normal human thyroids.

“…The mixture of sodium insulin at 1 pg/ml, bovine transferrin at 5 ug/ml, 0.01 mM hydrocortisone, glycyl-L-histidyl-L-lysine acetate (Sigma) at 10 ng/ml, and somatostatin (Sigma) at 10 ng/ml has been designated as 5H mixture or, when 10-9 M bovine TSH (Sigma) was added, as 6H. Freshly frozen bovine hypothalamus and bovine pituitary (Pel Freez Biologicals) extracts were added to a final concentration of 75 and 5 ug of protein per ml, respectively, and prepared as previously described (12,13 h at 37°C in 0.5 ml per well of medium with no thymidine, containing 0.1% bovine serum albumin (Janssen), [3H]thymidine (Amersham) at 2.5 jkCi/ml (1 uCi = 37 kBq), no insulin or insulin at S yg/ml, and no TSH or added bovine TSH at concentrations ranging from 10-8 M to 10-10 M. At the end ofincubation, cells were washed twice with Ca2+-and Mg2+-free HBSS and twice with 0.5 ml of ice-cold 10%1 trichloroacetic acid per well. After removal of supernatants, 0.5 ml of 2% sodium dodecyl sulfate was added per well, and 10 min later supernatants were collected to be analyzed for incorporated [3H]thymidine by liquid scintillation spectroscopy.…”

“…We now report the development of such cultures. This achievement has been made possible by substantial modifications of the culture medium: our formulation is largely based on a further modification of the F-12 modified medium (mF-12) previously developed by us for the rat thyroid cells (12,13 …”