Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells

Chandrasekar, Swetha; Kuipa, Sophia; Vargas, Ana I.; Ignatova, Tetyana; Rotkin, Slava V.; Jedlicka, Sabrina S.

doi:10.1016/j.bpr.2022.100061

Cited by 5 publications

(7 citation statements)

References 81 publications

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“…The fitted result gives three parameters, including Hill coefficient 𝑛 , Michaelis constant 𝑘 M , and maximum cellular uptake mass 𝑚 ̅ max . The deduced Hill coefficient 𝑛 at ~1.87, which is much larger than 1, reveals the positively cooperative uptake nature, or the active internalization process, such as receptor-mediated endocytosis of (6,5) into RAW macrophages [39][40][41][42] . The Michaelis constant 𝑘 M at 192.5 µg L −1 estimates the required (6,5) concentration to treat the cells for obtaining half of the maximum cellular (6,5) mass.…”

Section: 𝑚 ̅ (Fg Cellmentioning

confidence: 94%

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Cytometry in the short-wave infrared

Lin,

Liu,

Wang

et al. 2024

Preprint

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Cytometry plays a crucial role in characterizing cell properties, but its restricted optical window (400-850 nm) limits the number of stained fluorophores that can be detected simultaneously and hampers the study and utilization of short-wave infrared (SWIR; 900-1,700 nm) fluorophores in cells. Here we introduce two SWIR-based methods to address these limitations: SWIR flow cytometry and SWIR image cytometry. We develop a quantification protocol for deducing cellular fluorophore mass. Both systems achieve a limit of detection of ~0.1 fg cell−1 within a 30-min experimental timeframe, using individualized, high-purity (6,5) single-wall carbon nanotubes as a model fluorophore and macrophage-like RAW264.7 as a model cell line. This high-sensitivity feature reveals that low-dose (6,5) serves as an antioxidant, and cell morphology and oxidative stress dose-dependently correlate with (6,5) uptake. Our SWIR cytometry holds immediate applicability for existing SWIR fluorophores and offers a solution to the issue of spectral overlapping in conventional cytometry.

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Section: 𝑚 ̅ (Fg Cellmentioning

confidence: 94%

“…Figure 2c reveals the cellular (6,5) mass 𝑚 ̅ with respect to the (6,5) dose 𝐷 for the gated singlet-cells. This relation can be fitted well using the following Hill equation, which has been used to explain the nanoparticle uptake behaviors 38,39 :…”

Section: 𝑚 ̅ (Fg Cellmentioning

confidence: 99%

Cytometry in the short-wave infrared

Lin,

Liu,

Wang

et al. 2024

Preprint

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“…This led to a series of studies related to mechanisms of ssDNA-functionalized SWCNT uptake in cells, which revealed that internalization occurs via endocytosis, which is followed by an endosomal escape, and leads to SWCNT accumulation in the cytosol (Figure ). − …”

Section: Cnm Fluorescent Probe Applicationsmentioning

confidence: 99%

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Krasley,

Li,

Galeana

et al. 2024

Chem. Rev.

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Fluorescent carbon nanomaterials have broadly useful chemical and photophysical attributes that are conducive to applications in biology. In this review, we focus on materials whose photophysics allow for the use of these materials in biomedical and environmental applications, with emphasis on imaging, biosensing, and cargo delivery. The review focuses primarily on graphitic carbon nanomaterials including graphene and its derivatives, carbon nanotubes, as well as carbon dots and carbon nanohoops. Recent advances in and future prospects of these fields are discussed at depth, and where appropriate, references to reviews pertaining to older literature are provided.

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“…Plants incubated for the 5 days and then washed were also imaged after GUS staining using a confocal Raman microscope, − and the strong G-band Raman signature of SWCNTs at 1589 cm –1 was detected in frond tissue (Figure A–C); no Raman signature of SWCNTs was found in the rhizoid structures (Figure D). Thus, we conclude that single-walled CNTs facilitate the passive introduction of plasmid DNA into S. polyrhiza fronds, where transgenes can be transiently expressed.…”

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confidence: 99%

The “Duckweed Dip”: Aquatic Spirodela polyrhiza Plants Can Efficiently Uptake Dissolved, DNA-Wrapped Carbon Nanotubes from Their Environment for Transient Gene Expression

Islam,

Kalkar,

Tinker-Kulberg

et al. 2023

ACS Synth. Biol.

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Duckweeds (Lemnaceae) are aquatic nongrass monocots that are the smallest and fastest-growing flowering plants in the world. While having simplified morphologies, relatively small genomes, and many other ideal traits for emerging applications in plant biotechnology, duckweeds have been largely overlooked in this era of synthetic biology. Here, we report that Greater Duckweed (Spirodela polyrhiza), when simply incubated in a solution containing plasmid-wrapped carbon nanotubes (DNA-CNTs), can directly uptake the DNA-CNTs from their growth media with high efficiency and that transgenes encoded within the plasmids are expressed by the plants�without the usual need for large doses of nanomaterials or agrobacterium to be directly infiltrated into plant tissue. This process, called the "duckweed dip", represents a streamlined, "hands-off" tool for transgene delivery to a higher plant that we expect will enhance the throughput of duckweed engineering and help to realize duckweed's potential as a powerhouse for plant synthetic biology.

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Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells

Cited by 5 publications

References 81 publications

Cytometry in the short-wave infrared

Cytometry in the short-wave infrared

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

The “Duckweed Dip”: Aquatic Spirodela polyrhiza Plants Can Efficiently Uptake Dissolved, DNA-Wrapped Carbon Nanotubes from Their Environment for Transient Gene Expression

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