Cell cycle imaging with quantitative differential interference contrast microscopy

Kostyk, Piotr; Phelan, Shelley A.; Xu, Min

doi:10.1117/12.2005540

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…Tools such as phase contrast, differential interference contrast (DIC), and fluorescence microscopy are in widespread use in bioimaging applications. While not chemically specific, quantitative phase contrast (4-7) and DIC (8,9) can be used, apart from structural investigations, to quantitatively determine dry mass distribution in cell biology studies. On the other hand, fluorescence based methods which are chemically specific due to the selectivity of fluorophore binding sites can be applied in several ways, some of them offering super-resolution down to the 10 nm range.…”

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confidence: 99%

Quantitative Imaging of B1 Cyclin Expression Across the Cell Cycle Using Green Fluorescent Protein Tagging and Epifluorescence

et al. 2020

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In this article, we report the number of cyclin B1 proteins tagged with enhanced green fluorescent protein (eGFP) in fixed U-2 OS cells across the cell cycle. We use a quantitative analysis of epifluorescence to determine the number of eGFP molecules in a nondestructive way, and integrated over the cell we find 10 4 to 10 5 molecules. Based on the measured number of eGFP tagged cyclin B1 proteins, knowledge of cyclin B1 dynamics through the cell cycle, and the cell morphology, we identify the stages of cells in the cell cycle.

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confidence: 99%

Quantitative Imaging of B1 Cyclin Expression Across the Cell Cycle Using Green Fluorescent Protein Tagging and Epifluorescence

et al. 2020

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“…20 A fundamental process underlying the proliferation of cell populations, the cell cycle has been studied using methods such as fluorescence microscopy , 21 quantitative phase contrast , 22,23 and DIC . 24 These methods are either invasive (introduction of extraneous labels as in fluorescence microscopy) or lack chemical specificity. The cell cycle comprises two stages, interphase and mitosis.…”

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confidence: 99%

Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Karuna

Masia

Wiltshire

et al. 2019

Multiphoton Microscopy in the Biomedical Sciences XIX

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Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering," ABSTRACT Quantitative determination of the chemical composition of unstained samples, non-invasively, with high threedimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

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Cell cycle imaging with quantitative differential interference contrast microscopy

Cited by 2 publications

References 10 publications

Quantitative Imaging of B1 Cyclin Expression Across the Cell Cycle Using Green Fluorescent Protein Tagging and Epifluorescence

Quantitative Imaging of B1 Cyclin Expression Across the Cell Cycle Using Green Fluorescent Protein Tagging and Epifluorescence

Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

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