Cell cycle length, cell size, and proliferation rate in hydra stem cells

Holstein, Thomas W.; David, Charles N.

doi:10.1016/0012-1606(90)90360-u

Cited by 54 publications

(39 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Continuous Phylostratigraphic analysis of genes associated with cancer in humans indicates their early evolutionary emergence and predicts the evolutionary origin of animal tumours to lie in the root of metazoan tree 6 . This does not preclude the possibility that tumour suppressor-related genes in early metazoan evolution were involved in the control of cell proliferation to adjust to environmental changes 7 . labelling revealed that although interstitial cells from control polyps show the well-described labelling dynamics 26 with B80% cells labelled after 24 h (Fig. 3b), the proportion of labelled cells from tumour tissue is only B60%, indicating that in tumours lower fraction of cells have entered cell cycle.…”

Section: Morphology and Histologymentioning

confidence: 75%

Naturally occurring tumours in the basal metazoan Hydra

et al. 2014

View full text Add to dashboard Cite

The molecular nature of tumours is well studied in vertebrates, although their evolutionary origin remains unknown. In particular, there is no evidence for naturally occurring tumours in pre-bilaterian animals, such as sponges and cnidarians. This is somewhat surprising given that recent computational studies have predicted that most metazoans might be prone to develop tumours. Here we provide first evidence for naturally occurring tumours in two species of Hydra. Histological, cellular and molecular data reveal that these tumours are transplantable and might originate by differentiation arrest of female gametes. Growth of tumour cells is independent from the cellular environment. Tumour-bearing polyps have significantly reduced fitness. In addition, Hydra tumours show a greatly altered transcriptome that mimics expression shifts in vertebrate cancers. Therefore, this study shows that spontaneous tumours have deep evolutionary roots and that early branching animals may be informative in revealing the fundamental mechanisms of tumorigenesis.

show abstract

Section: Morphology and Histologymentioning

confidence: 75%

Naturally occurring tumours in the basal metazoan Hydra

et al. 2014

View full text Add to dashboard Cite

show abstract

“…RESULTS AND DISCUSSION composition of hydra tissue was determined in macerated preparations (David, 1973) as described previously (Holstein and David, 1990). …”

Section: Identification Of Cell Types By Maceration the Cellmentioning

confidence: 99%

“…The cell size of interstitial cells and their nuclei was analyzed by measuring the area of cells in macerated preparations as described by Holstein and David (1990). Since the cells are effectively flat under the coverslip (thinner than 4 #m), the area of a given cell is a good approximation of its volume.…”

Section: Identification Of Cell Types By Maceration the Cellmentioning

confidence: 99%

“…Leitz Dialux microscope fitted with a Leitz epifluorescence attachment using filter block A (365/450 nm) as described by Holstein and David (1990). (44 Ci/ mmole,Amersham) by injecting the isotope into the gastric cavity using a polyethylene needle (David and Campbell, 1972) as described (Holstein and David, 1990).…”

Section: Determination Of Nuclear Dna Content the Fluorescence Of Numentioning

confidence: 99%

“…(44 Ci/ mmole,Amersham) by injecting the isotope into the gastric cavity using a polyethylene needle (David and Campbell, 1972) as described (Holstein and David, 1990).…”

Section: Determination Of Nuclear Dna Content the Fluorescence Of Numentioning

confidence: 99%

See 2 more Smart Citations

Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

Holstein¹,

David²

1990

Developmental Biology

Self Cite

View full text Add to dashboard Cite

We have investigated the properties of nerve cell precursors in hydra by analyzing the differentiation and proliferation capacity of interstitial cells in the peduncle of Hydra oligactis, which is a region of active nerve cell differentiation.Our results indicate that about 50% of the interstitial cells in the peduncle can grow rapidly and also give rise to nematocyte precursors when transplanted into a gastric environment. If these cells were committed nerve cell precursors, one would not expect them to differentiate into nematocytes nor to proliferate apparently without limit. Therefore we conclude that cycling interstitial cells in peduncles are not intermediates in the nerve cell differentiation pathway but are stem cells. The remaining interstitial cells in the peduncle are in G1 and have the properties of committed nerve cell precursors . Thus, the interstitial cell population in the peduncle contains both stem cells and noncycling nerve precursors. The presence of stem cells in this region makes it likely that these cells are the immediate targets of signals which give rise to nerve cells.

show abstract

Bovine papillomavirus E1 protein affects the host cell cycle phase fractions

1994

View full text Add to dashboard Cite

C127 murine fibroblast cells were electroporated with a bovine papillomavirus E l protein expression vector and exariiined by flow cytometry. E l expressing cells (El + ) within the total cell population were distinguished from nonexpressing cells (El-) by immunofluorescent staining with anti-El serum and a fluorescein-conjugated second antibody. Under conditions of saturation with the first and second antibodies, the specific green fluorescence reflected the level of intracellular E l protein. Simultaneous staining with a DNA-specific dye, propidium iodide (PI), enabled the cell cycle distributions for the E l + and E l -cell populations to be determined. It was found that the E l + subpopulation had a reduced percentage of cells in G1 phase and an increased percentage of G2+M phase cells, compared to the E l -subpopulation. There was no significant difference in overall doubling time or percentage of noncycling cells in the E l + vs. E 1-populations, indicating that the change in cell cycle distribution was not due to a general activation or inhibition of cell growth by El. Direct measurement of cell cycle phase fractions confirmed that the G1 phase was decreased and the G2+M phase was increased in E l expressing cells. As these observations were made in the absence of other viral proteins or viral DNA replication, it suggests that the E l protein exerts an effect on the host cell independent of its direct role in viral DNA replication. Thus, E l may interact directly with the host cell cycle regulatory machinery. o 1994 Wiley-Liss, Inc.Key terms: Bovine papillomavirus, E l protein, cell cycle Bovine papillomavirus type 1 (BPV-1) is a small tumor virus with a 7945 bp double-strand DNA genome. Infection with BPV-1 causes benign proliferation of epithelial and fibroblastic cells resulting in the formation of fibropapillomas in cattle (19). Both the dermal and epithelial components of the wart maintain the viral DNA as a replicating extrachromosomal plasmid (22).Vegetative replication of the proviral DNA is induced by terminal differentiation of the basal cells of the epithelium. Although vegetative replication does not occur except in the host animal, portions of the infection process can be mimicked in culture. When BPV-1 DNA is introduced into cultured mouse fibroblast cells, there is an initial transient amplification of viral DNA, presumably similar to what occurs in natural infection (2,3,26,27). Ultimately, however, amplification of the viral genome ceases and stable transformants can be obtained (2,3,26,27). The BPV-1 DNA in these transformants is maintained as a multicopy nuclear plasmid a t a relative stable copy number. The ability of BPV-1 DNA to be both transiently amplified and stably maintained in culture has led to its use as a prototype for the analysis of papillomavirus DNA replication and regulation. In addition, the maintenance of a stable copy number implies a n intimate and coordinated regulation of viral DNA replication with host genomic replication. Examination of the mechanisms by which B...

show abstract

Cell cycle length, cell size, and proliferation rate in hydra stem cells

Cited by 54 publications

References 28 publications

Naturally occurring tumours in the basal metazoan Hydra

Naturally occurring tumours in the basal metazoan Hydra

Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

Bovine papillomavirus E1 protein affects the host cell cycle phase fractions

Contact Info

Product

Resources

About