Cell cycle regulation of H2b histone octamer DNA-binding activity in Chinese hamster lung fibroblasts.

Ito, Masamichi; Sharma, Ajay; Lee, Amy; Maxson, Rob

doi:10.1128/mcb.9.2.869

Cited by 27 publications

(15 citation statements)

References 33 publications

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“…) and contradicts the conclusions of Maxson and colleagues (Ito et al 1989) derived from experiments using extracts from serum-stimulated Chinese hamster lung fibroblasts. One obvious explanation for this differ ence is that the serum-stimulation protocol cannot be used to assess mechanisms operative in cycling cell pop ulations.…”

Section: Binding Of Subtype-specific Transcription Factors To the H2bcontrasting

confidence: 51%

“…Because the cell-cycle regulatory activity of the H2b (Fletcher et al 1987) and HI (this study) subtype-specific sequences have been reproduced in vitro, it was important to determine whether the DNA binding activities of the factors interacting with these elements change during the cell cycle. This seemed particularly important given the apparently con flicting results obtained in studies of the H2b subtypespecific factor OTFl-binding activity in extracts from serum-stimulated Chinese hamster lung fibroblasts (Ito et al 1989) and in extracts of chicken erythroid cells synchronized by a single treatment with aphidicolin (Dalton and Wells 1988b). Because neither of these pre- vious studies demonstrated by functional assays of the extracts that histone gene transcriptional regulation is reproduced in the extracts and because both studies em ployed less physiologic synchronization procedures, we decided to re-examine this issue in the elutriated cell extracts.…”

Section: Binding Of Subtype-specific Transcription Factors To the H2bmentioning

confidence: 99%

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Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro.

Bella

Gallinari²,

McKinney³

et al. 1989

Genes & Development

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In this study we used nuclear extracts from centrifugally elutriated cell populations to study histone HI transcriptional regulation during the cell cycle. Analysis of mutations within the HI promoter establish that both of the HI subtype-specific consensus elements participate in induction of transcription upon entry into S phase. The DNA binding activity of H1TF2, which specifically interacts with the HI proximal subtype-specific element, is increased in S-phase nuclear extracts, whereas no increase in DNA binding is observed for the HI distal subtype-specific DNA transcription factor HlTFl or the H2b subtype-specific factor OTFl. These data strongly support the idea that histone gene subtype-specific transcription factors are important for S-phasedependent expression of histone genes. Further studies of these factors will be important for increased understanding of the transition from G^ to S phase of the mammalian cell cycle.[Key Words: Cell cycle; histone genes; in vitro transcription; centrifugal elutriation; transcription factors] Received July 11, 1989; revised version accepted September 7, 1989.The transition from Gi to S phase in the eukaryotic cell cycle results in the activation of chromosomal DNA synthesis and the increased production of a variety of activities required for synthesis or packaging of nascent DNA. The synthesis of most histone proteins is dramat ically induced at this time (Robbins and Borum 1967), because of both increased rates of transcription of the individual histone genes and accelerated post-transcriptional histone mRNA accumulation (Heintz et al. 1983;Sittman et al. 1983). Recent studies of the mechanisms regulating histone gene transcription demonstrated that promoter proximal DNA sequences (Artishevsky et al. 1987;Dalton and Wells 1988a;La Bella et al. 1988) and their cognate transcription factors (Fletcher et al. 1987;Dalton and Wells 1988b) are crucial for increased tran scription during S phase. It is our belief that a detailed knowledge of these proteins and their changing activi ties during S phase can lead to fundamental advances in our understanding of the cell cycle.The most specific information concerning the regula tion of histone gene expression during S phase has been obtained by analysis of histone H2b gene expression. We have demonstrated that a very highly conserved sub type-specific consensus element, containing the core octanucleotide ATTTGCAT and positioned immediately upstream from the TATA element, mediates cell-cycle regulation of H2b transcription (La Bella et al. 1988). The transcription factor that specifically interacts with this regulatory element has been purified and characterized ^Corresponding author. (Fletcher et al. 1987), although the precise mechanism by which this factor contributes to the S-phase induc tion of H2b transcription is not yet known. The H2b cell-cycle regulatory element is not present in genes en coding other histone subtypes. However, comparison of the H4, H2b, and HI promoters reveals that each pro moter contains a highly co...

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Section: Binding Of Subtype-specific Transcription Factors To the H2bcontrasting

confidence: 51%

Section: Binding Of Subtype-specific Transcription Factors To the H2bmentioning

confidence: 99%

Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro.

Bella

Gallinari²,

McKinney³

et al. 1989

Genes & Development

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“…Octl DNA binding properties have demonstrated that Octl binding activity does not change in actively growing cells as they transit the cell cycle (7,15) but is depressed in cells that are arrested by serum starvation (14,15 (4,5). However, it is quite clear that this factor does not interact with several of the cloned human histone H4 promoters, suggesting that it probably is not critical for cell cycle regulation of the H4 gene family (unpublished data).…”

Section: Resultsmentioning

confidence: 99%

Histone gene transcription factor binding in extracts of normal human cells.

Bella¹,

Heintz²

1991

Mol. Cell. Biol.

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“…Indeed, it is interesting that OTF-1 is also required for cell cycle-regulated histone H2B transcription (40) and one study has indicated that OTF-1 levels increase upon stimulation of cell proliferation (41). Thus, in a quiescent cell that adenovirus would normally infect in vivo, the level of available OTF-1 might be low and thus limiting for viral replication.…”

Section: Discussionmentioning

confidence: 99%

DNA octamer element can confer E1A trans-activation, and adenovirus infection results in a stimulation of the DNA-binding activity of OTF-1/NFIII factor.

Chellappan

Nevins

1990

Proc. Natl. Acad. Sci. U.S.A.

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Adenovirus ElA-dependent trans-activation of viral transcription involves the utilization and alteration of multiple sequence-specific transcription factors. Cellular genes are also activated by ElA, one example being the immunoglobulin heavy-chain locus when assayed by transfection into fibroblast cells. We have explored the basis for the E1A-dependent activation of this cellular transcription unit. We demonstrate that the ATTTGCAT ("octamer") element found in the heavy-chain enhancer and promoter is a target for ElA trans-activation since this sequence can confer inducibility to the normally unresponsive simian virus 40 early promoter. In addition, adenovirus infection stimulates the DNA-binding activity of the ubiquitous octamer-specific factor, OTF-1, and we presume that this is the basis for the stimulation of transcription. Although there are no octamer elements in the adenovirus genome that are known to be important for transcription, there are octamer elements in the viral terminal repeat sequences. These elements bind the NFIH factor and are important for the initiation ofDNA replication. Since the NFIII factor has been shown to be identical to OTF-1, we suggest that the stimulation of OTF-1/NFUI activity during an adenovirus infection may be important for efficient initiation of adenovirns DNA synthesis.

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Cell cycle regulation of H2b histone octamer DNA-binding activity in Chinese hamster lung fibroblasts.

Cited by 27 publications

References 33 publications

Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro.

Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro.

Histone gene transcription factor binding in extracts of normal human cells.

DNA octamer element can confer E1A trans-activation, and adenovirus infection results in a stimulation of the DNA-binding activity of OTF-1/NFIII factor.

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