Cell cycle-related genes associate with sensitivity to hydrogen peroxide-induced toxicity

Bekeschus, Sander; Liebelt, Grit; Menz, Jonas; Singer, Debora; Wende, Kristian; Schmidt, Anke

doi:10.1016/j.redox.2022.102234

Cited by 12 publications

(8 citation statements)

References 69 publications

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“…H 2 O 2 is one of the most common types of ROS. 52 53,54 In this study, we found that the OSO challenge significantly increased the piglet liver H 2 O 2 content and markedly decreased SOD and CAT activities. This is similar to the findings in our prior research that maternal exposure to OSO resulted in higher H 2 O 2 levels and lower activity of CAT and SOD in the liver of offspring, 30 whereas Res supplementa- tion in the OSO group improved the content of H 2 O 2 and restored the CAT and SOD activities.…”

Section: ■ Discussionsupporting

confidence: 51%

“…Therefore, we also investigated the effects of OSO and Res on the hepatic antioxidant capacity. H 2 O 2 is one of the most common types of ROS . SOD catalyzes dismutation of O 2 – into H 2 O 2 and O 2 , while CAT neutralizes H 2 O 2 , producing H 2 O and O 2 . , In this study, we found that the OSO challenge significantly increased the piglet liver H 2 O 2 content and markedly decreased SOD and CAT activities.…”

Section: Discussionsupporting

confidence: 50%

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Dietary Resveratrol Ameliorates Hepatic Fatty Acid Metabolism and Jejunal Barrier in Offspring Induced by Maternal Oxidized Soybean Oil Challenge

Gao,

Liu,

et al. 2024

J. Agric. Food Chem.

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Increasing evidence indicates that maternal exposure to oxidized soybean oil (OSO) causes damage to the mother and offspring. The antioxidant resveratrol (Res) has a variety of health benefits. However, the protective effect of Res on mitigating offspring damage after maternal exposure to OSO and its mechanism remains unclear. Therefore, this study aimed to investigate the effect of Res on hepatic fatty acid metabolism and the jejunal barrier in suckling piglets after maternal OSO exposure. A total of 18 sows in late gestation were randomly assigned to three treatments. The sows were fed with a fresh soybean oil (FSO) diet, an OSO diet, or the OSO diet supplemented with 300 mg/kg Res (OSO + Res), respectively. The results showed that maternal supplementation of Res restored the mRNA levels of genes related to fatty acid metabolism and increased the activities of catalase (CAT) and total superoxide dismutase (T-SOD) in suckling piglets' livers under the OSO challenge. Moreover, the OSO + Res group restored the mRNA levels of occludin and claudin 4 in suckling piglet jejunum compared with the results of the OSO challenges. In summary, supplementation with Res improves hepatic fatty acid metabolism and intestinal barrier function of suckling piglets after maternal OSO challenge during late gestation and lactation.

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Section: ■ Discussionsupporting

confidence: 51%

Section: Discussionsupporting

confidence: 50%

Dietary Resveratrol Ameliorates Hepatic Fatty Acid Metabolism and Jejunal Barrier in Offspring Induced by Maternal Oxidized Soybean Oil Challenge

Gao,

Liu,

et al. 2024

J. Agric. Food Chem.

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“…CKAP5 encodes a protein involved in microtubule organization and dynamics, which are crucial for various cellular processes, including cell division and response to cellular stressors, including oxidative stress 60,61 . MCM5 and RAD21 are involved in DNA repair mechanisms, which are common in cells exposed to toxic agents 58,62 . CDC6 plays a role in DNA replication initiation and is regulated in response to cellular stress induced by toxicants 63 .…”

Section: Resultsmentioning

confidence: 99%

“…The biggest change was for PHF13 (chr1:6623607), which increased from 26% U-to-C error in the untreated library to 56% U-to-C error following Pb 2+ treatment. Among these, six sites are found to be on genes involved in cellular processes that occur when cells are exposed to toxic molecules 58,59 . In particular, FBXO5 encodes a protein involved in the ubiquitin-proteasome system, which is crucial for protein degradation and cellular response to various stresses, including exposure to toxic molecules such as chromium 59 .…”

Section: Sh-sy5y Cell State Changes In Response To Lead (Pb 2+ ) Expo...mentioning

confidence: 99%

Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity

Fanari,

Tavakoli,

Qiu

et al. 2024

Preprint

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Chemical modifications in mRNAs such as pseudouridine (psi) can regulate gene expression, although our understanding of the functional impact of individual psi modifications, especially in neuronal cells, is limited. We apply nanopore direct RNA sequencing to investigate psi dynamics under cellular perturbations in SH-SY5Y neuroblastoma cells. We identified psi sites and assigned them to psi synthase enzymes using siRNA-based knockdown, finding that TRUB1 knockdown slightly upregulates psi occupancy in PUS7 sites. Next, we studied psi occupancy changes upon differentiation and lead exposure of SH-SY5Y cells. A simple steady-state enzyme-substrate model reveals a strong correlation between psi synthase and mRNA substrate levels and psi modification frequencies. Finally, comparing psi sites across all three cellular states reveals stable and plastic modifications that are either condition-dependent or condition-independent. Our psi occupancy analysis across cell states, integrated with knockdown validation of modification sites, allows robust investigations into the dynamics and plasticity of RNA modifications.

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“…A study has shown that the expression of SEL1L3 is elevated in endometrial cancer. In white patients with low mutation load, the expression level of this gene is related to the patient’s recurrence free productivity and is considered as a potential driver and tumor marker of endometrial cancer ( 27 ).. SEL1L3 was also positively correlated with reactive oxygen species such as hydrogen peroxide ( 28 ). An elegant series of studies found that the VDR signaling affect tumor development by the delicate interplay with E-cadherin and the Wnt signaling pathway ( 29 – 32 ).…”

Section: Discussionmentioning

confidence: 99%

Effective oxygen metabolism-based prognostic signature for colorectal cancer

Yuan

Zhang²,

Ma³

et al. 2023

Front. Oncol.

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BackgroudOxygen metabolism is an important factor affecting the development of tumors, but its roles and clinical value in Colorectal cancer are not clear. We developed an oxygen metabolism (OM) based prognostic risk model for colorectal cancer and explored the role of OM genes in cancer.MethodsGene expression and clinical data obtained from The Cancer Genome Atlas, Clinical Proteomic Tumor Analysis Consortium databases were consider as discovery and validation cohort, respectively. The prognostic model based on differently expressed OM genes between tumor and GTEx normal colorectal tissues were constructed in discovery cohort and validated in validation cohort. The Cox proportional hazards analysis was used to test clinical independent. Upstream and downstream regulatory relationships and interaction molecules are used to clarify the roles of prognostic OM genes in colorectal cancer.ResultsA total of 72 common differently expressed OM genes were detected in the discovery and validation set. A five-OM gene prognostic model including LRT2, ATP6V0E2, ODC1, SEL1L3 and VDR was established and validated. Risk score determined by the model was an independent prognostic according to routine clinical factors. Besides, the role of prognostic OM genes involves transcriptional regulation of MYC and STAT3, and downstream cell stress and inflammatory response pathways.ConclusionsWe developed a five-OM gene prognostic model and study the unique roles of oxygen metabolism in of colorectal cancer

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Cell cycle-related genes associate with sensitivity to hydrogen peroxide-induced toxicity

Cited by 12 publications

References 69 publications

Dietary Resveratrol Ameliorates Hepatic Fatty Acid Metabolism and Jejunal Barrier in Offspring Induced by Maternal Oxidized Soybean Oil Challenge

Dietary Resveratrol Ameliorates Hepatic Fatty Acid Metabolism and Jejunal Barrier in Offspring Induced by Maternal Oxidized Soybean Oil Challenge

Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity

Effective oxygen metabolism-based prognostic signature for colorectal cancer

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