Cell cycle status of CD34+ cells in human fetal bone marrow

Koenig, Joyce M.; Luttge, Benjamin G.; Benson, Neal A.; Christensen, Robert D.

doi:10.1016/s0378-3782(01)00226-2

Cited by 10 publications

(4 citation statements)

References 11 publications

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“…These observations are very similar to findings in humans, in whom Ͻ0.5% of CD34 ϩ cells have been reported in the peripheral circulation and 1-4% in bone marrow (33,(43)(44)(45). Our observations also agree with findings in humans, in whom cord blood CD34 ϩ cells have been shown to possess different characteristics when compared with cells that are obtained from fetal or adult bone marrow (33)(34)(35)46).…”

Section: Cd14supporting

confidence: 90%

Effect of Age on the Frequency, Cell Cycle, and Lineage Maturation of Rhesus Monkey (Macaca mulatta) CD34+ and Hematopoietic Progenitor Cells

2005

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The effects of maturation and aging on hematopoietic progenitor cells, blood and bone marrow from second-and thirdtrimester fetal, newborn, infant, adult, and aged rhesus monkeys (Macaca mulatta) were analyzed. CD34 ϩ cells were immunoselected and stained with propidium iodide for cell cycle analysis. Blood and bone marrow mononuclear cells were plated in methylcellulose, and erythroid and myeloid progenitors were grown and counted. A higher frequency of circulating CD34 Somatic cells possess a limited ability for self-renewal, as described in the study of cellular aging by Hayflick (1). This intrinsic capability or "Hayflick limit" of cell division, also described as self-renewal potential and proliferation of somatic cells, is believed to decline in chronologically older cells (2). Unlike somatic cells, CD34ϩ hematopoietic stem cells (HSCs) are a long-lived population that can sustain blood cell production for the lifetime of an individual. A balance among selfrenewal, proliferation, differentiation, and migration of pluripotent HSCs is the hallmark of hematopoiesis. For example, Andrews et al. (3) showed that the CD34 ϩ population, which contains HSCs, is responsible for hematopoietic reconstitution of lethally irradiated baboons, whereas others have shown that a small number of HSCs from the bone marrow of C57BL/6 mice can repopulate lymphohematopoietic tissues of irradiated animals (4,5). However, in mouse studies, a greater quantity of HSCs was required for reconstitution when donor HSCs were obtained from older versus younger mice. In similar studies, HSCs that were obtained from fetal liver have shown greater long-term repopulating capability when compared with HSCs from adult marrow. HSCs from DBA/2, BALB/c, and CBA/ CaH-T6 mice indicate a significant functional decline with advancing age (6 -11), and transplant studies suggest that murine HSCs from a single donor can be serially transplanted only to five to seven recipients (12-14). These observations suggest that HSCs may possess finite self-renewal capabilities and are subject to the Hayflick limit.A recent stochastic study on the replication, apoptosis, and differentiation of HSCs has revealed that mouse, cat, and nonhuman primate HSCs are biologically different in their intrinsic capabilities and that findings in the mouse may not represent in vivo behavior of HSCs in large animals or humans (15). Other studies in baboons and cats concur with this observation (6,16 -18

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Section: Cd14supporting

confidence: 90%

Effect of Age on the Frequency, Cell Cycle, and Lineage Maturation of Rhesus Monkey (Macaca mulatta) CD34+ and Hematopoietic Progenitor Cells

2005

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“…What is clear is that fetal/neonatal cells appear to cycle spontaneously at a higher rate than their adult counterparts. For example, a higher percentage of freshly isolated fetal CD34 ϩ hemopoietic stem cells appear to be in cycle compared with the adult CD34 ϩ population (32,33). This spontaneous proliferation is not limited to hemopoietic cells.…”

Section: Discussionmentioning

confidence: 99%

Murine Neonatal Lymphocytes Show Rapid Early Cell Cycle Entry and Cell Division

Adkins

Williamson

Guevara

et al. 2003

The Journal of Immunology

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Neonatal animals are highly susceptible to infectious agents. At least part of this susceptibility is due to the virtual absence of immunological memory in newborns. One of the hallmarks of memory is the rapidity of the response. We show in this study that neonates may make up for their lack of memory, at least in part, by the rapid entry of large proportions of naive lymphocytes into the cell cycle. Following activation, greater percentages of both CD4+ and CD8+ neonatal, as compared with adult, lymph node cells showed early cell cycle entry; this was assessed by propidium iodide staining, CFSE labeling profiles, [3H]thymidine uptake, and up-regulation of early activation markers. This rapid cycle entry was observed following polyclonal activation with anti-CD3 or with PMA and ionomycin and in both C57BL/6 and BALB/c mice. Stimulation with specific peptide also elicited more rapid proliferative responses from neonatal vs adult TCR transgenic CD4+ cells. In addition, more rapid cycle entry was observed in vivo, in lymphopenic RAG2−/− hosts. For both CD4+ and CD8+ cells, this phenomenon was observed out to 3 wk of life, although the differences between neonatal and adult cells became smaller with increasing time postbirth. These properties of peripheral neonatal T cells appeared to be inherited from their thymic precursors, because CD4+8− single-positive cells in the neonatal thymus also showed more rapid cycle entry, compared with their counterparts in the adult thymus. Interestingly, rapid early cycling was also observed among activated neonatal B cells, compared with adult B cells. Thus, early cell cycle entry by large proportions of cells may allow the naive lymphocyte population to efficiently mobilize responses against the broad range of pathogens first encountered in neonatal life.

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“…36 Furthermore, cycling rates of hematopoietic cells and absolute numbers of immature and mature circulating neutrophils are elevated in neonates compared with adults. 37,38 Neutrophil counts at 12 hours after birth range from 7,000 to 15,000/mcL in term infants, and at times are higher in preterm neonates compared with children and adults. 38,39 Complement protein concentrations increase after birth and approximate mean adult values between 6 and 18 months of age.…”

Section: Neutrophils and Complement During The Neonatal Periodmentioning

confidence: 99%

“…37,38 Neutrophil counts at 12 hours after birth range from 7,000 to 15,000/mcL in term infants, and at times are higher in preterm neonates compared with children and adults. 38,39 Complement protein concentrations increase after birth and approximate mean adult values between 6 and 18 months of age. 40 Reference values of C1r, C2, C5, C7, Properdin, and factors D, H, and I and C3a and C5a have been determined in cord blood samples from healthy term newborns.…”

Section: Neutrophils and Complement During The Neonatal Periodmentioning

confidence: 99%

Dysfunction of innate immunity and associated pathology in neonates

Petrová

Mehta

2007

Indian J Pediatr

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The neutrophils and complement system are the critical elements of innate immunity mainly due to participation in the first line of defense against microorganisms by means of phagocytosis, lysis of bacteria and activation of naive B-lymphocytes. In this report we provide an overview of the up to date information regarding the neutrophil and complement system's functional ability in newborn infants in association with the maternal conditions that exist during the intrauterine stage, gestational age and post-neonatal pathology. The neonates' capacity to control the neutrophil and complement protein activation process has also been discussed because of the evidence that uncontrolled activation of these immune elements provides a significant contribution to the tissue damage and subsequent pathology. The authors are confident that despite the many unanswered questions this review updates their knowledge and points the need for further research to clarify the role of the age-associated dysfunction of neutrophils and complement system in the infection and inflammation related pathology of newborn infants.

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Cell cycle status of CD34+ cells in human fetal bone marrow

Abstract: 2016-12-26T14:57:54

Cited by 10 publications

References 11 publications

Effect of Age on the Frequency, Cell Cycle, and Lineage Maturation of Rhesus Monkey (Macaca mulatta) CD34+ and Hematopoietic Progenitor Cells

Effect of Age on the Frequency, Cell Cycle, and Lineage Maturation of Rhesus Monkey (Macaca mulatta) CD34+ and Hematopoietic Progenitor Cells

Murine Neonatal Lymphocytes Show Rapid Early Cell Cycle Entry and Cell Division

Dysfunction of innate immunity and associated pathology in neonates

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