Cell death due to electroporation – A review

Napotnik, Tina Batista; Polajžer, Tamara; Miklavčič, Damijan

doi:10.1016/j.bioelechem.2021.107871

Cited by 262 publications

(200 citation statements)

References 260 publications

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“…After 3–5 days, a quality check of the cell’s health and the expression levels of GFP was performed under the two-photon microscope through a cranial window ( Figure 1C ). If only a single neuron per mouse expressed GFP and it did not show any signs of dendritic “blebbing” or cell death (Batista Napotnik et al, 2021 ), the animal was transcardially perfused with PFA in order to fix the brain. Subsequently, the extracted brain was partially cleared using mCUBIC (Susaki et al, 2015 ) and prepared for two-photon serial section tomographic 3D imaging (Han et al, 2018 ; Figure 1D ).…”

Section: Resultsmentioning

confidence: 99%

Axonal and Dendritic Morphology of Excitatory Neurons in Layer 2/3 Mouse Barrel Cortex Imaged Through Whole-Brain Two-Photon Tomography and Registered to a Digital Brain Atlas

et al. 2022

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Communication between cortical areas contributes importantly to sensory perception and cognition. On the millisecond time scale, information is signaled from one brain area to another by action potentials propagating across long-range axonal arborizations. Here, we develop and test methodology for imaging and annotating the brain-wide axonal arborizations of individual excitatory layer 2/3 neurons in mouse barrel cortex through single-cell electroporation and two-photon serial section tomography followed by registration to a digital brain atlas. Each neuron had an extensive local axon within the barrel cortex. In addition, individual neurons innervated subsets of secondary somatosensory cortex; primary somatosensory cortex for upper limb, trunk, and lower limb; primary and secondary motor cortex; visual and auditory cortical regions; dorsolateral striatum; and various fiber bundles. In the future, it will be important to assess if the diversity of axonal projections across individual layer 2/3 mouse barrel cortex neurons is accompanied by functional differences in their activity patterns.

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Section: Resultsmentioning

confidence: 99%

Axonal and Dendritic Morphology of Excitatory Neurons in Layer 2/3 Mouse Barrel Cortex Imaged Through Whole-Brain Two-Photon Tomography and Registered to a Digital Brain Atlas

et al. 2022

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“…Finally, lipid rafts polarize, which consequently induces intracellular activities that modulate cell migration. 20,22,54,55…”

Section: Electrostimulation Of Cells: Influence Of Electrical Cues On...mentioning

confidence: 99%

Electro-responsive polymer-based platforms for electrostimulation of cells

Kanaan

Piedade

2022

Mater. Adv.

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“…This type of electroporation is referred to as irreversible (IRE). 40,51 In electroporation, three general levels have been defined: (1) no detectable electroporation; (2) reversible electroporation; and (3) IRE. The range over which each of these occur is characterized by the strength of the external electric field applied (V/cm) and the duration of exposure (seconds) to it.…”

Section: Principles Of Plasma-membrane Electroporationmentioning

confidence: 99%

“…These cells then lose their integrity, with the release of their contents, and ultimately die. 51,53 From a mechanistic point of view, electroporation is best described by the theory of hydrophilic pore formation. The external electric field induces a drop in the electric potential across the lipid bilayer, which leads to the formation of hydrophilic pores in the bilayer.…”

Section: Principles Of Plasma-membrane Electroporationmentioning

confidence: 99%

The Good and the Bad of Cell Membrane Electroporation

Balantič

Miklavčič

Križaj

et al. 2021

ACSi

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Electroporation is used to increase the permeability of the cell membrane through high-voltage electric pulses. Nowadays, it is widely used in different areas, such as medicine, biotechnology, and the food industry. Electroporation induces the formation of hydrophilic pores in the lipid bilayer of cell membranes, to allow the entry or exit of molecules that cannot otherwise cross this hydrophobic barrier. In this article, we critically review the basic principles of electroporation, along with the advantages and drawbacks of this method. We discuss the effects of electroporation on the key components of biological membranes, as well as the main applications of this procedure in medicine, such as electrochemotherapy, gene electrotransfer, and tissue ablation. Finally, we define the most relevant challenges of this romising area of research.

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Cell death due to electroporation – A review

Cited by 262 publications

References 260 publications

Axonal and Dendritic Morphology of Excitatory Neurons in Layer 2/3 Mouse Barrel Cortex Imaged Through Whole-Brain Two-Photon Tomography and Registered to a Digital Brain Atlas

Axonal and Dendritic Morphology of Excitatory Neurons in Layer 2/3 Mouse Barrel Cortex Imaged Through Whole-Brain Two-Photon Tomography and Registered to a Digital Brain Atlas

Electro-responsive polymer-based platforms for electrostimulation of cells

The Good and the Bad of Cell Membrane Electroporation

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