Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations

Lunov, Oleg; Zablotskii, V.; Churpita, O.; Chánová, Eliška Mázl; Syková, Eva; Dejneka, A.; Kubinová, Šárka

doi:10.1038/srep07129

Cited by 77 publications

(74 citation statements)

References 62 publications

(98 reference statements)

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“…This finding is in line with our previous results that plasmainduced biological effects are cell/bacteria type dependent. 8 The efficacy of bacteria deactivation was highly dependent on a distance between the plasma nozzle and bacteria culture (Fig. 2(c)).…”

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confidence: 97%

“…8,9 However, the mechanisms of these effects and factors related to regulation of bacteria proliferation and death have been poorly investigated and not fully understood. Interactions between bacteria (cells) and plasmas can be carried out by means of any of plasma's agents: neutral gas, charged particles, excited atoms/molecules, reactive species (such as NO, O 3 , OH, and O 2 À ), UV light, electrical field, and ion bombarding.…”

mentioning

confidence: 99%

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Non-thermal plasma mills bacteria: Scanning electron microscopy observations

Lunov

Churpita

Zablotskii

et al. 2015

Applied Physics Letters

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Non-thermal plasmas hold great promise for a variety of biomedical applications. To ensure safe clinical application of plasma, a rigorous analysis of plasma-induced effects on cell functions is required. Yet mechanisms of bacteria deactivation by non-thermal plasma remain largely unknown. We therefore analyzed the influence of low-temperature atmospheric plasma on Gram-positive and Gram-negative bacteria. Using scanning electron microscopy, we demonstrate that both Grampositive and Gram-negative bacteria strains in a minute were completely destroyed by helium plasma. In contrast, mesenchymal stem cells (MSCs) were not affected by the same treatment. Furthermore, histopathological analysis of hematoxylin and eosin-stained rat skin sections from plasma-treated animals did not reveal any abnormalities in comparison to control ones. We discuss possible physical mechanisms leading to the shred of bacteria under non-thermal plasma irradiation. Our findings disclose how helium plasma destroys bacteria and demonstrates the safe use of plasma treatment for MSCs and skin cells, highlighting the favorability of plasma applications for chronic wound therapy. V C 2015 AIP Publishing LLC. [http://dx.doi.org/10.1063/1.4907624]Deactivation of bacteria and control of cellular nanomechanics can be done by different physical factors, including: mechanical forces, 1 laser irradiation, 2 high-gradient magnetic field, 3-5 pulses of electric field, 6 ultrasound, 7 and atmospheric non-thermal plasma. 8,9 However, the mechanisms of these effects and factors related to regulation of bacteria proliferation and death have been poorly investigated and not fully understood. Interactions between bacteria (cells) and plasmas can be carried out by means of any of plasma's agents: neutral gas, charged particles, excited atoms/molecules, reactive species (such as NO, O 3 , OH, and O 2 À ), UV light, electrical field, and ion bombarding. Thus, various constituents of plasma can affect biological, physical, and chemical processes in mammalian cell cultures, tissue, and microorganisms.In the last several decades, the problem of antibiotic resistance emerged as a rapidly growing public health issue. 10 Therefore, there is an indispensable need for the design and development of novel alternatives to antibiotics. In this regard, non-thermal plasma represents an important and effective alternative approach in overcoming resistant microorganisms. 11,12 The use of non-thermal atmospheric pressure plasma devices in clinical practice requires operating regimes that can be easily controlled and adjusted during treatments. Moreover, some side effects of the developed plasma devices, in particular, on the respiratory organs or the overheating of healthy tissues, can be expected. These unfavorable effects can be reduced by tuning of the physical properties of plasma generators. A burst of studies indicate a great potential of low temperature plasmas use in a wide variety of biological and medical applications, 12 specifically in wound healing. 9,13 Concomitantl...

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confidence: 97%

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confidence: 99%

Non-thermal plasma mills bacteria: Scanning electron microscopy observations

Lunov

Churpita

Zablotskii

et al. 2015

Applied Physics Letters

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“…Reactive-nitrogen- and -oxygen-based species play an important role in the toxic effect of PAW, and have DNA, RNA, proteins, and lipids as principal targets2021. Moreover, both non-thermal plasma and PAW could catalyze the generation of intracellular ROS, consequently causing oxidative stress in bacterial cells222324.…”

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confidence: 99%

Bactericidal Effects against S. aureus and Physicochemical Properties of Plasma Activated Water stored at different temperatures

Shen

Tian

et al. 2016

Sci Rep

266

222

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Water activated by non-thermal plasma creates an acidified solution containing reactive oxygen and nitrogen species, known as plasma-activated water (PAW). The objective of this study was to investigate the effects of different storage temperatures (25 °C, 4 °C, −20 °C, −80 °C) on bactericidal activities against S. aureus and physicochemical properties of PAW up to 30 days. Interestingly, PAW stored at −80 °C yielded the best antibacterial activity against Staphylococcus aureus, 3~4 log reduction over a 30-day period after PAW generation; meanwhile, PAW stored at 25 °C, 4 °C, and −20 °C, respectively, yielded 0.2~2 log decrease in cell viability after the same exposure and storage time. These results were verified by scanning electron microscope (SEM). The physicochemical properties of PAW stored at different temperatures were evaluated, including pH, oxidation reduction potential (ORP), and hydrogen peroxide, nitrate, nitrite anion and NO radical levels. These findings suggested that bacterial activity of PAW stored at 25 °C, 4 °C, −20 °C decreased over time, and depended on three germicidal factors, specifically ORP, H2O2, and NO3−. Moreover, PAW stored at −80 °C retained bactericidal activity, with NO2− contributing to bactericidal ability in association with H2O2. Our findings provide a basis for PAW storage and practical applications in disinfection and food preservation.

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“…Increasing evidence suggests that reactive oxygen species (ROS) are the major players in NTAPP-induced apoptosis in vitro 131415. However, there is a discrepancy between the cytotoxic effect of non-thermal plasma and ozone, which is a considerable component of non-thermal air plasma1617.…”

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confidence: 99%

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

Park

Lee

et al. 2016

Sci Rep

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Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.

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Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations

Cited by 77 publications

References 62 publications

Non-thermal plasma mills bacteria: Scanning electron microscopy observations

Non-thermal plasma mills bacteria: Scanning electron microscopy observations

Bactericidal Effects against S. aureus and Physicochemical Properties of Plasma Activated Water stored at different temperatures

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

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