Cell‐Derived Extracellular Matrix‐Rich Biomimetic Substrate Supports Podocyte Proliferation, Differentiation, and Maintenance of Native Phenotype

Abstract

Current technologies and available scaffold materials do not support longterm cell viability, differentiation, and maintenance of podocytes, the ultraspecialized kidney resident cells that are responsible for the filtration of the blood. A new platform which imitates the native kidney microenvironment by decellularizing fibroblasts grown on surfaces with macromolecular crowding is developed. Human immortalized podocytes cultured on this platform display superior viability and metabolic activity up to 28 days compared to podocytes cultured on tissue culture plastic surfaces. The new platform displays a softer surface and an abundance of growth factors and associated molecules. More importantly, it enables podocytes to display molecules responsible for their structure and function and a superior development of intercellular connections/interdigitations, consistent with maturation. The new platform can be used to study podocyte biology, test drug toxicity, and determine whether sera from patients with podocytopathies are involved in the expression of glomerular pathology.

Tmprss2 specific miRNAs as promising regulators for SARS-CoV-2 entry checkpoint

“…In addition, viral intervention targeting Tmprss2 has been demonstrated in several studies from recent reports in the mouse models as well as human cell lines with knockdown of Tmprss2 exhibiting a reduced viral invasion ( Bestle et al, 2020 ; Glowacka et al, 2011 ; Iwata-Yoshikawa et al, 2019 ; Stopsack et al, 2020 ; Strope et al, 2020 ). Tmprss2 has shown additional involvement in the activation of several extracellular matrix associated substrates in certain cancers ( Satyam et al, 2020 ). Studies have informed the stimulating actions on matriptase from Tmprss2 facilitating prostate cancer growth and progression.…”

Porous Polymeric Microspheres With Controllable Pore Diameters for Tissue Engineered Lung Tumor Model Development

“…A pair t-test with two-tailed distribution yielded a significant difference with p-value = 0.04, and this confirmed our hypothesis that overall MRC-5 and A549 grow more on collagen-coated PPMS than those on non-coated PPMS. Recently, there has been an alternative method wherein cell-derived decellularized matrix (DCM) produced by human fibroblasts improves cellular differentiation and function with more realistic in vivo micking model (Satyam et al, 2020). We propose to use this concept as the replacement of collagen in our future experiments.…”

3D Printed Biomimetic Rabbit Airway Simulation Model for Nasotracheal Intubation Training

“…The weakest feature of the model was the difference in realism of nasal cartilage and mucosa due to the elastic consistency of the Tango Plus material. With the availability of bioprinting technology, organs and tissues can be directly printed with a combination of bioinks that provide the possibility to replicate the biomechanical properties of tissues more accurately (52,53). However, the aim of the current study was to develop models that can be repeatedly used to train nasotracheal intubation for a longer duration, which hence could be a concern with bioprinted models.…”