Cell-derived microparticles after exercise in individuals with G6PD Viangchan

Abstract

Glucose-6-phospate dehydrogenase (G6PD) deficient cells are sensitive to oxidative damage leading to the formation of microparticles (MPs). Therefore, we examined the concentration of MPs and changes in the antioxidant balance after an acute strenuous exercise (SEx) and moderate-intensity exercise (MEx). Eighteen healthy females (18-24 years) with G6PD normal and eighteen age-matched females with G6PD Viangchan (871G>A) were tested by running on a treadmill at their maximal oxygen uptake for SEx and at 75% of their maximal heart rate for MEx. It was found that SEx triggered the release of total microparticles (TTMPs) above baseline levels and remained significantly higher 45 minutes after the exercise in G6PD normal individuals. However, SEx-induced increase in TTMPs was significantly higher in G6PD Viangchan as compared to G6PD normal. In contrast, MEx did not to alter the release of TTMPs in both G6PD normal and Viangchan. Moreover, TTMPs concentrations were inversely correlated with G6PD activity (r =-0.82, P < 0.05) but positively correlated with MDA concentrations (r = 0.74, P < 0.05). Using cell specific antibodies, we determined that MPs were mainly derived from platelets and erythrocytes. Altogether, the present study indicates that G6PD Viangchan may participate in MEx without higher MPs concentration and oxidative stress compared with G6PD normal.

Effects of an acute bout of exercise on circulating extracellular vesicles: tissue-, sex-, and BMI-related differences

“…Differently from the results of other studies, in which no difference or an increase in postexercise platelet-derived EVs (particularly, CD41a+ or CD42b+ EVs) was found [19,35,36], a decrease in CD61+ EVs occurred in our study population after acute exercise. Interestingly, this effect was more evident at 24h after the end of the test.…”

Systematic review of the clinical manifestations of glucose-6-phosphate dehydrogenase deficiency in the Greater Mekong Subregion: implications for malaria elimination and beyond

“…Other findings on the clinical manifestations of G6PDd were described in four articles. 69–72 A study in Thailand found that among females with the G6PD Viangchan mutation, strenuous exercise induced a significantly higher increase in total microparticle level (an indicator of oxidative damage) compared with age-matched G6PD normal females. 69 In a study from Thailand, the authors suggested that excessive fluoride in drinking water might worsen the effect of iron deficiency anaemia, thalassemia and G6PDd.…”

“… 69–72 A study in Thailand found that among females with the G6PD Viangchan mutation, strenuous exercise induced a significantly higher increase in total microparticle level (an indicator of oxidative damage) compared with age-matched G6PD normal females. 69 In a study from Thailand, the authors suggested that excessive fluoride in drinking water might worsen the effect of iron deficiency anaemia, thalassemia and G6PDd. 71 In another study from Thailand, among 165 neonates, G6PDd was the main reason 22 neonates (13.4%) required exchange transfusion therapy.…”

Extracellular Vesicles: A Novel Target for Exercise-Mediated Reductions in Type 2 Diabetes and Cardiovascular Disease Risk

“…Subsequently, understanding the acute exercise effect on EVs provides insight independent of cardiorespiratory fitness adaptation and weight/fat loss. However, to date there are limited studies examining the effects of acute aerobic [ 60 , 61 ] or resistance exercise [ 62 ] on EVs ( Table 1 ). For instance, Mobius-Winkler et al tested the effect of a 4 hr cycling protocol at 70% of the anaerobic threshold in 18 young, lean, healthy males [ 63 ] and found no change in endothelial EVs (CD42b − , CD42b − /CD62E + ) in the immediate postexercise period, despite increases in cytokine IL-6.…”

“…Nonetheless, these findings are consistent with Guiraud et al who showed that there was no change in endothelial EVs (CD31 + , CD62E + , and CD42b − ) or platelet EVs (CD42b + ) in 19 male coronary heart disease patients when measured up to 72 hr following either high-intensity interval or moderate-intensity cycling exercise [ 60 ]. In contrast, Chanda et al reported that a maximal bout of exercise (defined as a VO 2 max test) elicited an approximate 40% increase in platelet (CD41a) EVs in healthy adults [ 61 ]. While these later findings suggest that exercise intensity raises EV, it should be noted that maximal exercise would be considered a stressful perturbation to the system and it is known that high-intensity exercise raises oxidative stress and inflammation in the immediate postexercise period, thereby conferring a stimulus for metabolic adaptation [ 64 ].…”