Cell differentiation in the preimplantation human embryo

Hansis, Christoph; Edwards, Robert G.

doi:10.1016/s1472-6483(10)61712-9

Cited by 46 publications

(13 citation statements)

References 34 publications

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“…In human blastomeres, the distribution of marker genes such as Oct-4 and β- HCG indicates that their allocation follows this specific cleavage pattern (Cauffman et al ., 2005; Hansis 2006) and those blastomeres might differ in their potency. The nature of early blastomere differentiation in the human 4-cell embryo and its consequences for embryonic development has been discussed and analysed (for reviews, see Edwards and Beard, 1997, 1999; Hansis and Edwards, 2003; Edward and Hansis, 2005). …”

Section: Discussionmentioning

confidence: 99%

Assisted fertilization and embryonic axis formation in higher primates

Piotrowska-Nitsche

Yang

Banta³

et al. 2009

Reproductive BioMedicine Online

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In naturally fertilized embryos of various organisms, the spermatozoon provides a localized cue to initiate early embryonic patterning. In mice, the sperm entry point (SEP) may reorient the first cleavage division, which separates the zygote into two halves that follow distinct fates. However, it is unknown whether the mechanical injection of spermatozoa into an oocyte by intracytoplasmic sperm injection (ICSI), a technique commonly used in human assisted reproduction, possesses such a role. Rhesus macaque embryos fertilized by ICSI were examined in order to determine the consequences of placing the spermatozoon at specific positions in the ooplasm and whether this can provide new information about patterning in mammalian eggs. The SEP specified by the injected spermatozoa was most often localized near the first cleavage plane and was mainly distributed along the boundary zone that separates the embryonic and abembryonic parts of the monkey blastocyst. Moreover, the ICSI data, when compared with naturally fertilized mouse embryos, showed a similar outcome in terms of cleavage axes and first embryonic axis specification. As there are no studies to date regarding sperm entry in human oocytes and its influence on embryonic development, this investigation using the rhesus macaque as a clinical model is noteworthy.

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Section: Discussionmentioning

confidence: 99%

Assisted fertilization and embryonic axis formation in higher primates

Piotrowska-Nitsche

Yang

Banta³

et al. 2009

Reproductive BioMedicine Online

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“…Indeed, cDNA microarray analysis of 3 different hESC lines showed no less than 30%-50% of all genes exhibiting differential expression patterns [94], and greater than 100-fold variations in lineagespecific gene expression were found in another study comparing 17 hESC lines [95]. Subtle molecular allocations of individual ICM cells to developmental fates more specific than the rather broad epiblast and primitive endoderm categories may be an important cause for these phenomena [45,46,48] as is the case for mice [96][97][98][99].…”

Section: Discussionmentioning

confidence: 99%

“…We derived 12 independent, genetically diverse, and stable hESC lines (NYUES1-12) from discard embryos ( Table 1) taking advantage of methods developed in our lab [30,33,[45][46][47][48][49]. The lines were derived from 59 embryos from 18 in vitro fertilization (IVF) cycles.…”

Section: Derivation Of Hesc Linesmentioning

confidence: 99%

Derivation of Novel Genetically Diverse Human Embryonic Stem Cell Lines

Stefanova

Grifo²,

Hansis³

2012

Stem Cells and Development

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Human embryonic stem cells (hESCs) have the potential to revolutionize many biomedical fields ranging from basic research to disease modeling, regenerative medicine, drug discovery, and toxicity testing. A multitude of hESC lines have been derived worldwide since the first 5 lines by Thomson et al. 13 years ago, but many of these are poorly characterized, unavailable, or do not represent desired traits, thus making them unsuitable for application purposes. In order to provide the scientific community with better options, we have derived 12 new hESC lines at New York University from discarded genetically normal and abnormal embryos using the latest techniques. We examined the genetic status of the NYUES lines in detail as well as their molecular and cellular features and DNA fingerprinting profile. Furthermore, we differentiated our hESCs into the tissues most affected by a specific condition or into clinically desired cell types. To our knowledge, a number of characteristics of our hESCs have not been previously reported, for example, mutation for alpha thalassemia X-linked mental retardation syndrome, linkage to conditions with a genetic component such as asthma or poor sperm morphology, and novel combinations of ethnic backgrounds. Importantly, all of our undifferentiated euploid female lines tested to date did not show X chromosome inactivation, believed to result in superior potency. We continue to derive new hESC lines and add them to the NIH registry and other registries. This should facilitate the use of our hESCs and lead to advancements for patient-benefitting applications.

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“…Another explanation could be provided by the fact that only extra-embryonic (trophectoderm) cells are biopsied. In contrast, the lineage specific developmental fate of an individual blastomere is unpredictable by morphology (23). Finally, it is possible that blastocysts possess increased tolerance to manipulation compared with cleavage-stage embryos as a result of having already undergone embryonic genome activation (20).…”

Section: What Is the Best Time To Perform Biopsy?mentioning

confidence: 99%

Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives

Capalbo¹

2015

cce

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Cell differentiation in the preimplantation human embryo

Cited by 46 publications

References 34 publications

Assisted fertilization and embryonic axis formation in higher primates

Assisted fertilization and embryonic axis formation in higher primates

Derivation of Novel Genetically Diverse Human Embryonic Stem Cell Lines

Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives

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