Cell disruption for microalgae biorefineries

Günerken, Emre; Dhondt, Elisabeth; Eppink, M.H.M.; Garcia‐Gonzalez, Linsey; Elst, Kathy; Wijffels, René H.

doi:10.1016/j.biotechadv.2015.01.008

Cited by 629 publications

(374 citation statements)

References 148 publications

(151 reference statements)

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“…Silva et al (2014) applied a microwave pretreatment (400 W, 4 min) on mixed culture biomass of Chlorophyceae, Cyanophyceae, Euglenophyceae, and Bacillariophyceae and reported a remarkably higher lipid extraction (33.7%) compared to the untreated sample (4.8%). Advantages of the microwave technology include effectiveness, robustness, and easy scale-up, but effectiveness depends largely on the type of microalgae (G€ unerken et al, 2015;Passos et al, 2015b).…”

Section: Physical Methods For Cell Wall Disruptionmentioning

confidence: 99%

“…Ultrasonic cavitation at low (18-40 kHz) frequency is much stronger than at high frequency (400-800 kHz). Nevertheless, its efficiency depends on the microalgae species (shape, size, intracellular structure), operational conditions (temperature, time, power, number of cycles), and biomass concentration (G€ unerken et al, 2015;Passos et al, 2015b). Ultrasound microalgae pretreatment has been mainly applied for biodiesel, bioethanol, and biogas production and has been proven to adequately break algal cells in low concentration biomass suspension.…”

Section: Physical Methods For Cell Wall Disruptionmentioning

confidence: 99%

“…Several recent studies have discussed the cost-effectiveness of the previously mentioned physical-mechanical disruption technologies (Greenwell et al, 2010;Balasundaram et al, 2012;Coons et al, 2014;Al hattab and Ghaly, 2015;Artan et al, 2015;G€ unerken et al, 2015). Yet generalization is very complicated due to the high variety of methods and many unknowns.…”

Section: Cost-effectiveness Of Disruption Technologiesmentioning

confidence: 99%

“…Doucha and Lívanský (2008) demonstrated that energy consumption could be reduced from 10.3 to 0.86 kWh/kg by altering bead milling parameters. Mechanical methods Power consumption was recalculated based on data from Artan et al (2015), Balasundaram et al (2012), Coons et al (2014), andG€ unerken et al (2015).…”

Section: Cost-effectiveness Of Disruption Technologiesmentioning

confidence: 99%

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Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

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IntroductionAlgal cell walls separate the inside cell content from the environment to protect the cell against desiccation, pathogens, and predators while still allowing exchange of compounds. Toward application of algae biomass as a sustainable resource, disruption of this cell wall (¼cell disruption) is an essential pretreatment step to maximize product recovery in downstream processes of the algae biorefinery. Also for direct use of algae in feed or food, cell rupture is required to increase the bioavailability of algae constituents. Depending on the cell wall structure, the size, and the shape of algae, cell disruption can be challenging. A variety of cell disruption methods is currently available, and new approaches are being elaborated in parallel. Since downstream processing is responsible for a large part of the operational costs in the whole production chain, cell disruption technologies should be low cost and energy efficient and result preferably in high product quality. This chapter provides information on cell wall types and gives an overview of physical-mechanical and (bio-)chemical cell disruption technologies with attention to development stage, energy efficiency, product quality, costs, emerging approaches, and applicability on large scale. Cell wall types in various groups of microalgae and cyanobacteriaThe cell wall composition and architecture of algae and cyanobacteria are highly variable ranging from tiny membranes to multilayered complex structures. Despite the importance of algal cell wall properties in biotechnology, little structural information is available for most species (Scholz et al., 2014). Based on the complexity of surface structures, four cell types could be distinguished (Barsanti and Gualtieri, 2006;Lee, 2008) (Fig. 6.1). A simple cell membrane (Fig. 6.1, Type 1) is present in short-lived stages (e.g., gametes), chrysophytes, raphidophytes, green algae Dunaliella, and haptophytes Isochrysis. It consists of a lipid bilayer with integrated and peripheral proteins. Sometimes a cap of glycolipids and glycoproteins envelops the outer surface of cell membrane. Cell membranes with additional extracellular material are known in cyanobacteria and many groups of algae, including palmelloid phases. It is the most diverse cell wall type that includes various membrane-associated structures (cell wall, Microalgae-Based Biofuels and Bioproducts. http://dx

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Section: Physical Methods For Cell Wall Disruptionmentioning

confidence: 99%

Section: Physical Methods For Cell Wall Disruptionmentioning

confidence: 99%

Section: Cost-effectiveness Of Disruption Technologiesmentioning

confidence: 99%

Section: Cost-effectiveness Of Disruption Technologiesmentioning

confidence: 99%

See 2 more Smart Citations

Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, alternative mechanical methods such as tangential and ultrafiltration of proteins obtaining extraction efficiencies up to 76% of the solubilized proteins (Ursu et al, 2014) have also been studied, but they were discarded due to the high costs. Low-cost mechanical methods are still generally preferred for large-scale applications aimed to obtain high added value products (Günerken et al, 2015;Hernández et al, 2015). In this context, more conservative methods like sonication or high pressure cell disruption have been studied, reporting high protein solubilization (from 52.3 to 73.0%) of the total initial proteins in the microalga using pH 11 in Nannochloropsis sp.…”

Section: Introductionmentioning

confidence: 99%