Cell DNA content — Correlation with clonogenicity in the human tumour cloning system (HTCS)

Verheuen, R. H. M.; Feitz, W. F. J.; Beck, J. L. M.; Debruyne, F.M.J.; Vooys, G. P.; Kenemans, P.; Herman, Chester J.

doi:10.1002/ijc.2910350514

Cited by 12 publications

(4 citation statements)

References 22 publications

(11 reference statements)

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“…Among the diploid tumors which grew in soft agar, we cannot rule out the possibility that some of them contain a small aneuploid population which has not been detected. Comparable data were reported by Verheijen et al (1985). The amounts of ER and PR (fmoles/mg cell prot.)…”

Section: Discussionsupporting

confidence: 61%

Flow cytometric analysis of human breast tumors and assessment of in vitro chemosensitivity by clonogenic assays

et al. 1990

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We report a double-agar clonogenic system adapted to human breast cancer. We optimized the conditions for cell growth and clonogenicity with respect to hormones (insulin, estradiol, progesterone) and components of the extracellular matrix (collagen, laminin and fibronectin). Using our experimental improvements, 67% of the breast tumor samples received were grown successfully. Tests on 21 tumors with three agents: Doxorubicin, Methotrexate and 5-Fluorouracil permit objective discrimination of the in vitro pharmacosensitivity of human breast tumors. Flow cytometric analysis reveal that 64% of the tumors were diploid and 36% were aneuploid. The aneuploid tumors grew better in the double agar layer system used for the clonogenic assay. The diploid tumors were especially rich in estrogen (ER+) and progesterone (PR+) receptors whereas the aneuploid tumors were mostly estrogen and progesterone receptors negative (ER-/PR-). Finally, we noted no difference in drug responsiveness depending on the tumor ploidy and steroid receptor content.

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Section: Discussionsupporting

confidence: 61%

Flow cytometric analysis of human breast tumors and assessment of in vitro chemosensitivity by clonogenic assays

et al. 1990

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“…Flow cytometric analysis of renal-cell tumors offers a new diagnostic tool for the clinician and has additional prognostic value (Baisch et a l e , 1982; Herman et al, 1984). Tumors can be further subclassified on the basis of this technique and selection of patients at risk is made possible by detection of biologically important cell fractions (Ljungberg et al, 1985;Lovett et al, 1984;Otto et al, 1984;Schwabe et al, 1983;Verheyen et al, 1985). The use of tissue-specific markers, such as cytokeratins, in combination with DNA analysis in flow cytometry permits better analysis of tumor fractions within cell suspensions and may provide further information about subfractions in complex tumors (Feitz et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

Tissue‐specific markers in flow cytometry of urological cancers. II. Cytokeratin and vimentin in renal‐cell tumors

Feitz

Karthaus

Beck

et al. 1986

Intl Journal of Cancer

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Nine primary human renal-cell tumors (RCT), one lymph-node metastasis, 4 human xenografts of a RCT in nude mice and a rat RCT line were analyzed by flow cytometry (FCM) using propidium iodide for DNA analysis and antibodies to cytokeratin and vimentin in the indirect immunofluorescence technique for labelling of specific tumor-cell populations. By means of 2-dimensional FCM analysis, vimentin- and cytokeratin-positive (tumor) cells were compared and their DNA content and proliferative fraction analyzed separately from those of cytokeratin-negative stromal and inflammatory cells. In primary human RCT, 2 subpopulations of cells were detected and analyzed separately. Small numbers of tumor cells with an abnormal DNA stemline were also detected. In addition, co-expression of intermediate filament proteins of both the cytokeratin and the vimentin types was detected in the aneuploid cell population. Comparison of 2 model systems of RCT with primary human RCT revealed a similar pattern of tumor-cell subfractions within these tumors. The 2-parameter FCM analysis permits the detection of subpopulations in complex cell suspensions and the quantification of these fractions, as well as analysis of their cellular DNA content.

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“…For the detection of the growth potential of tumor cells in soft agar a modified two-layer soft agar culture method as originally described by Hamburger and Salmon [25] was used [26]. Growth potential was quantified using an Omnicon Fas I1 automated colony counter (Milton Roy, Rochester, NY, USA) [27].…”

Section: Human Tumor Colony-forming Assay (Htcs)mentioning

confidence: 99%

Effect of alpha‐ and gamma‐Interferon and tumor necrosis factor on colony formation of two human renal tumor xenografts in vitro

Beniers

Peelen

Hendriks

et al. 1988

Seminars in Surgical Oncology

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The in vitro antitumor activity of alpha- and gamma-interferon as well as tumor necrosis factor was tested on two human renal cell carcinoma xenografts--RC-43 and NC-65. A dose-dependent inhibition of colony formation in soft agar was found for both tumor lines. NC-65, however, showed no sensitivity for gamma-interferon. Combination drug tests showed a synergistic effect on colony formation of both tumor lines. The strongest inhibition was shown by the combination of alpha-interferon and tumor necrosis factor. We conclude that both interferons as well as tumor necrosis factor show direct in vitro cytostatic effects against renal tumor direct in vitro cytostatic effects against renal tumor cells.

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Cell DNA content — Correlation with clonogenicity in the human tumour cloning system (HTCS)

Cited by 12 publications

References 22 publications

Flow cytometric analysis of human breast tumors and assessment of in vitro chemosensitivity by clonogenic assays

Flow cytometric analysis of human breast tumors and assessment of in vitro chemosensitivity by clonogenic assays

Tissue‐specific markers in flow cytometry of urological cancers. II. Cytokeratin and vimentin in renal‐cell tumors

Effect of alpha‐ and gamma‐Interferon and tumor necrosis factor on colony formation of two human renal tumor xenografts in vitro

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