Cell dynamics in <scp>H</scp>ertwig's epithelial root sheath and surrounding mesenchyme in mice irradiated to the head

Ide, Yoshiaki; Nakahara, Taka; Nasu, Masanori; Ishikawa, Hiroshi

doi:10.1111/odi.12253

Cited by 6 publications

(2 citation statements)

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“…Both environmental and genetic conditions may impair root development. A study in mice showed that radiation of 10 and 20 Grey before the onset of root development disrupted the HERS and gave root malformation 17 . A genetic study using Nuclear factor I-C (Nfic) knockout mice demonstrated disturbances in root patterning leading to taurodontism in these mice 18 .…”

Section: Discussionmentioning

confidence: 99%

Molar incisor malformation in six cases: description and diagnostic protocol

Brusevold

Bie

Baumgartner

et al. 2017

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Section: Discussionmentioning

confidence: 99%

Molar incisor malformation in six cases: description and diagnostic protocol

Brusevold

Bie

Baumgartner

et al. 2017

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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“…Computerized 3D reconstruction was performed using 3D analytic software (TRI/3D-BON; Ratoc System Engineering, Tokyo, Japan). The first molars were extracted and spuriously stained upon the slice image, as described previously by Ide [ 24 ]. Thereafter, 3D reconstruction of the tooth images was performed.…”

Section: Methodsmentioning

confidence: 99%

Cyclophosphamide-Induced Morphological Changes in Dental Root Development of ICR Mice

2015

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BackgroundSurvivors of childhood cancer are at risk of late dental development. Cyclophosphamide is one of the most commonly used chemotherapeutic agents against cancer in children. The aim of this study was to investigate the effects of cyclophosphamide on root formation in the molars of growing mice and to assess the morphological changes in these roots using three-dimensional structural images.MethodsWe treated 16 12-day-old ICR mice with cyclophosphamide (100 mg/kg, i.p.) and 16 control mice with saline. At 16, 20, 24, and 27 days of age, the mandibular left first molars were scanned using soft micro-computed tomography. After scanning, the structural indices were calculated using a three-dimensional image analysis system, and the images were subjected to three-dimensional reconstruction. The length and apical foramen area of all distal roots were assessed. Histological changes in the apical region were then assessed via hematoxylin and eosin staining.ResultsThe mandibular molars of all experimental mice showed evidence of cytotoxic injury, which appeared in the form of anomalous root shapes. Although all roots developed further after cyclophosphamide injection, the three-dimensional structural images showed that the roots in the experimental group tended to develop more slowly and were shorter than those in the control group. At 27 days of age, the mean root length was shorter in the experimental group than in the control group. Conversely, the apical foramen of the roots in the experimental group tended to close faster than that of roots in the control group. In addition, hematoxylin and eosin staining of the distal roots in the experimental group showed increased dentin thickness in the apical region.ConclusionOur results suggest that cyclophosphamide can result in short root lengths and early apical foramen closure, eventually leading to V-shaped or thin roots.

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Preliminary study of improved median filter using adaptively mask size in light microscopic image

Kim

Lee

2020

Microscopy

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This study aimed to develop and evaluate an improved median filter (IMF) with an adaptive mask size for light microscope (LM) images. We acquired images of the mouse first molar using a LM at 100× magnification. The images obtained using our proposed IMF were compared with those from a conventional median filter. Several parameters such as the contrast-to-noise ratio, coefficient of variation, no-reference assessments and peak signal-to-noise ratio were employed to evaluate the image quality quantitatively. The results demonstrated that the proposed IMF could effectively de-noise the LM images and preserve the image details, achieving a better performance than the conventional median filter.

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Cell dynamics in Hertwig's epithelial root sheath and surrounding mesenchyme in mice irradiated to the head

Abstract: These results indicate that the early disappearance of HERS and the proliferative suppression of the surrounding mesenchymal cells, which was induced by irradiation, caused dental root malformation.

Cited by 6 publications

References 22 publications

Molar incisor malformation in six cases: description and diagnostic protocol

Molar incisor malformation in six cases: description and diagnostic protocol

Cyclophosphamide-Induced Morphological Changes in Dental Root Development of ICR Mice

Preliminary study of improved median filter using adaptively mask size in light microscopic image

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