Cell electrofusion using nanosecond electric pulses

Rems, Lea; Ušaj, Marko; Kandušer, Maša; Reberšek, Matej; Miklavčič, Damijan; Pucihar, Gorazd

doi:10.1038/srep03382

Cited by 120 publications

(97 citation statements)

References 60 publications

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“…2009; Rems et al. 2013). The material for cell cytoplasm was set to have an electric conductivity of 0.3 S m −1 and a relative permittivity of 80 (Pucihar et al.…”

Section: Methodsmentioning

confidence: 99%

“…2013). The AC/DC module of COMSOL incorporates this variable conductivity for the cell membrane conductivity (eq.…”

Section: Methodsmentioning

confidence: 99%

“…2013)Pore conductivity σ p 0.0745 S m −1 (Rems et al. 2013)Free diffusion coefficient D 0 5 × 10 −10 m −2 sec −1 (Towhidi and Miklavcic 2010)Diffusion coefficients for the interactive transport D p D 0 /5(Towhidi and Miklavcic 2010)Effective charge number (with sign) for DNA Z eff −1(Neumann et al. 1999)Elementary charge e 0 1.60 × 10 −19 C(Neumann et al.…”

mentioning

confidence: 99%

“…2009; Towhidi and Miklavcic 2010; Rems et al. 2013)Cell membrane electric conductivity (passive) σ m0 5 × 10 −7 S m −1 (Pucihar et al. 2009; Towhidi and Miklavcic 2010; Rems et al.…”

mentioning

confidence: 99%

“…2009; Rems et al. 2013)Characteristic voltage of electroporation V ep 170 mV(Pucihar et al. 2009; Rems et al.…”

mentioning

confidence: 99%

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Comparison between direct and reverse electroporation of cells in situ: a simulation study

et al. 2016

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The discovery of the human genome has unveiled new fields of genomics, transcriptomics, and proteomics, which has produced paradigm shifts on how to study disease mechanisms, wherein a current central focus is the understanding of how gene signatures and gene networks interact within cells. These gene function studies require manipulating genes either through activation or inhibition, which can be achieved by temporarily permeabilizing the cell membrane through transfection to deliver cDNA or RNAi. An efficient transfection technique is electroporation, which applies an optimized electric pulse to permeabilize the cells of interest. When the molecules are applied on top of seeded cells, it is called “direct” transfection and when the nucleic acids are printed on the substrate and the cells are seeded on top of them, it is termed “reverse” transfection. Direct transfection has been successfully applied in previous studies, whereas reverse transfection has recently gained more attention in the context of high‐throughput experiments. Despite the emerging importance, studies comparing the efficiency of the two methods are lacking. In this study, a model for electroporation of cells in situ is developed to address this deficiency. The results indicate that reverse transfection is less efficient than direct transfection. However, the model also predicts that by increasing the concentration of deliverable molecules by a factor of 2 or increasing the applied voltage by 20%, reverse transfection can be approximately as efficient as direct transfection.

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“…2009; Rems et al. 2013). The material for cell cytoplasm was set to have an electric conductivity of 0.3 S m −1 and a relative permittivity of 80 (Pucihar et al.…”

Section: Methodsmentioning

confidence: 99%

“…2013). The AC/DC module of COMSOL incorporates this variable conductivity for the cell membrane conductivity (eq.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

“…2009; Towhidi and Miklavcic 2010; Rems et al. 2013)Cell membrane electric conductivity (passive) σ m0 5 × 10 −7 S m −1 (Pucihar et al. 2009; Towhidi and Miklavcic 2010; Rems et al.…”

mentioning

confidence: 99%

“…2009; Rems et al. 2013)Characteristic voltage of electroporation V ep 170 mV(Pucihar et al. 2009; Rems et al.…”

mentioning

confidence: 99%

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Comparison between direct and reverse electroporation of cells in situ: a simulation study

et al. 2016

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Variation in dielectric properties due to pathological changes in human liver

Peyman

Kos

Djokić

et al. 2015

Bioelectromagnetics

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Dielectric properties of freshly excised human liver tissues (in vitro) with several pathological conditions including cancer were obtained in frequency range 100 MHz-5 GHz. Differences in dielectric behavior of normal and pathological tissues at microwave frequencies are discussed based on histological information for each tissue. Data presented are useful for many medical applications, in particular nanosecond pulsed electroporation techniques. Knowledge of dielectric properties is vital for mathematical calculations of local electric field distribution inside electroporated tissues and can be used to optimize the process of electroporation for treatment planning procedures.

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Nonlinear Dispersive Model of Electroporation for Irregular Nucleated Cells

Chiapperino

Bia²,

Caratelli³

et al. 2019

Bioelectromagnetics

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Cell electrofusion using nanosecond electric pulses

Cited by 120 publications

References 60 publications

Comparison between direct and reverse electroporation of cells in situ: a simulation study

Comparison between direct and reverse electroporation of cells in situ: a simulation study

Variation in dielectric properties due to pathological changes in human liver

Nonlinear Dispersive Model of Electroporation for Irregular Nucleated Cells

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