2009
DOI: 10.1007/s10544-009-9338-0
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Cell encapsulation and oxygenation in nanoporous microcontainers

Abstract: With strides in stem cell biology, cell engineering and molecular therapy, the transplantation of cells to produce therapeutic molecules endogenously is an attractive and achievable alternative to the use of exogenous drugs. The encapsulation of such cell transplants in semi-permeable, nanoporous constructs is often required to protect them from immune attack and to prevent their proliferation in the host. However, effective graft immunoisolation has been mostly elusive owing to the absence of a high-throughpu… Show more

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Cited by 16 publications
(22 citation statements)
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“…In vitro experiments showed that islet function is unimpaired after 48 h of encapsulation(181). Other experiments studied the diffusion of fluorescent probe molecules across the nanoporous membranes: IgG-FITC(182), lectin-FITC (140 kDa) and FM 4-64 (608 Da)(183). While FM 4-64 diffused without any problem, some diffusion of the larger molecules (that would ideally be blocked) was also observed.…”
Section: Current Immunoprotective Membrane Designsmentioning
confidence: 99%
See 1 more Smart Citation
“…In vitro experiments showed that islet function is unimpaired after 48 h of encapsulation(181). Other experiments studied the diffusion of fluorescent probe molecules across the nanoporous membranes: IgG-FITC(182), lectin-FITC (140 kDa) and FM 4-64 (608 Da)(183). While FM 4-64 diffused without any problem, some diffusion of the larger molecules (that would ideally be blocked) was also observed.…”
Section: Current Immunoprotective Membrane Designsmentioning
confidence: 99%
“…This may be attributed either to the slit shape of the nanopores, possibly enabling flexible proteins to cross the barrier, or to a gap between the lid and the base of the microcapsules (no experiment has been carried out yet to determine the degree of sealing of the assembly). In order to investigate cell oxygenation within these nanoporous containers, 9L rat glioma cells were engineered to bioluminesce under hypoxic conditions(182), which could potentially be used for future in vivo experiments. The preliminary results indicate that the nanoporous capsules may provide restricted oxygenation of the encapsulated cells.…”
Section: Current Immunoprotective Membrane Designsmentioning
confidence: 99%
“…And the silicon-based microcapsules showed cell viability and insulin production in response to glucose level after transplantation for eight days. We have reported development of biocompatible SU-8-based nanoporous microcontainers [7,8]. In this paper, we present a magnetic bonding of two pieces of SU-8-based nanporous microcontainers for cell transplantation devices.…”
Section: Antibodymentioning
confidence: 99%
“…The microcapsule was designed to house a single pancreatic islet, or a cluster of insulin-secreting cells of equivalent volume, with a 200 × 200 × 200-μm encapsulation space to ensure graft oxygenation. 14,15 The encapsulation space was in the base structure of the microcapsule, which was then closed with a nanoslotted lid. Several methods to assemble the components (base and lid) of these microcapsules that include geometric and magnetic assembly have been reported.…”
Section: An Examplementioning
confidence: 99%