Cell encapsulation in liquified compartments: Protocol optimization and challenges

Correia, Clara R.; Ghasemzadeh-Hasankolaei, Maryam; Mano, João F.

doi:10.1371/journal.pone.0218045

Cited by 28 publications

(27 citation statements)

References 29 publications

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“…This technique is booming in the research and industry fields because of its ability to produce monodisperse droplets from nano-size to hundreds of micrometers, depending on the processing parameters [ 109 , 110 ]. Several researchers have successfully employed this technique to encapsulate macromolecular bioactive agents such as cells [ 111 ], proteins [ 112 ], nucleic acids [ 46 ] and others. Firstly, a liquid is pumped slowly through a thin metal needle using a syringe pump by a constant flow rate.…”

Section: Alginate Nanoparticles Preparation Methodsmentioning

confidence: 99%

Alginate Nanoformulation: Influence of Process and Selected Variables

2020

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Nanocarriers are defined as structures and devices that are constructed using nanomaterials which add functionality to the encapsulants. Being small in size and having a customized surface, improved solubility and multi-functionality, it is envisaged that nanoparticles will continue to create new biomedical applications owing to their stability, solubility, and bioavailability, as well as controlled release of drugs. The type and physiochemical as well as morphological attributes of nanoparticles influence their interaction with living cells and determine the route of administration, clearance, as well as related toxic effects. Over the past decades, biodegradable polymers such as polysaccharides have drowned a great deal of attention in pharmaceutical industry with respect to designing of drug delivery systems. On this note, biodegradable polymeric nanocarrier is deemed to control the release of the drug, stabilize labile molecules from degradation and site-specific drug targeting, with the main aim of reducing the dosing frequency and prolonging the therapeutic outcomes. Thus, it is essential to select the appropriate biopolymer material, e.g., sodium alginate to formulate nanoparticles for controlled drug delivery. Alginate has attracted considerable interest in pharmaceutical and biomedical applications as a matrix material of nanocarriers due to its inherent biological properties, including good biocompatibility and biodegradability. Various techniques have been adopted to synthesize alginate nanoparticles in order to introduce more rational, coherent, efficient and cost-effective properties. This review highlights the most used and recent manufacturing techniques of alginate-based nanoparticulate delivery system, including emulsification/gelation complexation, layer-by-layer, spray drying, electrospray and electrospinning methods. Besides, the effects of the main processing and formulation parameters on alginate nanoparticles are also summarized.

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Section: Alginate Nanoparticles Preparation Methodsmentioning

confidence: 99%

Alginate Nanoformulation: Influence of Process and Selected Variables

2020

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“…Taking advantage of the mild and physical crosslinking of alginate, we have explored the production of multilayered and liquefied capsules containing microparticles for regenerative medicine purposes. [69,[111][112][113][114][115][116] The introduction of microparticles in the core environment act as anchorage points for cells, ensuring cell survival and proliferation, [111,69] and, if desired, cell differentiation. [112] Such strategy relies on the use of alginate templates for the encapsulation of cells and microparticles within an ultrathin LbL membrane.…”

Section: Liquefied Systemsmentioning

confidence: 99%

“…Additionally, combined with hydrodynamic atomization, an array of such liquefied-core capsules presenting different diameters in the micrometric range could be achieved at high rates (Figure 5B1). [114][115][116] We have also combined LbL with superhydrophobic-superhydrophilic microarrays or electrodeposition techniques to produce liquefied capsules with varying geometries (Figure 5B2). [117] Others, have developed compatible donut-and dumbbell-shaped liquefied microcapsules that enabled construct self-locking due to the LbLinduced shrinkage, followed by liquefaction-induced microcapsule swelling.…”

Section: Liquefied Systemsmentioning

confidence: 99%

Minimalist Tissue Engineering Approaches Using Low Material‐Based Bioengineered Systems

Correia

Bjørge

Nadine

et al. 2021

Adv Healthcare Materials

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From an “over‐engineering” era in which biomaterials played a central role, now it is observed to the emergence of “developmental” tissue engineering (TE) strategies which rely on an integrative cell‐material perspective that paves the way for cell self‐organization. The current challenge is to engineer the microenvironment without hampering the spontaneous collective arrangement ability of cells, while simultaneously providing biochemical, geometrical, and biophysical cues that positively influence tissue healing. These efforts have resulted in the development of low‐material based TE strategies focused on minimizing the amount of biomaterial provided to the living key players of the regenerative process. Through a “minimalist‐engineering” approach, the main idea is to fine‐tune the spatial balance occupied by the inanimate region of the regenerative niche toward maximum actuation of the key living components during the healing process.

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“…This method produces microparticles in a high throughput manner. In some studies, alginate microparticles, as the most prevalent biomaterials used for β -cells’ encapsulation, are produced by EHD spraying ( Jeyhani et al, 2018 ; Correia et al, 2019 ).…”

Section: Hydrogel-based Encapsulation Devicesmentioning

confidence: 99%

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Ghasemi

Akbari

Imani

2021

Front. Bioeng. Biotechnol.

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Islet transplantation provides a promising strategy in treating type 1 diabetes as an autoimmune disease, in which damaged β-cells are replaced with new islets in a minimally invasive procedure. Although islet transplantation avoids the complications associated with whole pancreas transplantations, its clinical applications maintain significant drawbacks, including long-term immunosuppression, a lack of compatible donors, and blood-mediated inflammatory responses. Biomaterial-assisted islet transplantation is an emerging technology that embeds desired cells into biomaterials, which are then directly transplanted into the patient, overcoming the aforementioned challenges. Among various biomaterials, hydrogels are the preferred biomaterial of choice in these transplants due to their ECM-like structure and tunable properties. This review aims to present a comprehensive overview of hydrogel-based biomaterials that are engineered for encapsulation of insulin-secreting cells, focusing on new hydrogel design and modification strategies to improve β-cell viability, decrease inflammatory responses, and enhance insulin secretion. We will discuss the current status of clinical studies using therapeutic bioengineering hydrogels in insulin release and prospective approaches.

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Cell encapsulation in liquified compartments: Protocol optimization and challenges

Cited by 28 publications

References 29 publications

Alginate Nanoformulation: Influence of Process and Selected Variables

Alginate Nanoformulation: Influence of Process and Selected Variables

Minimalist Tissue Engineering Approaches Using Low Material‐Based Bioengineered Systems

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

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