Cell encapsulation: Promise and progress

Orive, Gorka; Hernandez, Rufo Sánchez; Rodríguez-Gascón, Alicia; Calafiore, Riccardo; Chang, Thomas Ming Swi; Vos, Paul de; Hortelano, Gonzalo; Hunkeler, David; Lacı́k, Igor; Shapiro, A.M. James; Pedraz, José Luís

doi:10.1038/nm0103-104

Cited by 551 publications

(371 citation statements)

References 38 publications

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“…Cell encapsulation in self-assembled hydrogel matrix Encapsulation is a process entrapping living cells within the confines of a semi-permeable membrane or within a homogenous solid mass [66,86,87,113]. The biomaterials used for encapsulation are usually hydrogels, which are formed by covalent or ionic crosslinking of water-soluble polymers.…”

Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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“…This involves controlled systems, operational rigor and improved quality control. Monitoring and retrievability whether through genetic [83] , biochemical [84] or magnetic tagging [85] More interdisciplinary and integrated input is required to realise this ambitious goal incorporating expertise from the fields of physicochemistry, tissue engineering, cell biology, pharmaceutical technology, materials science, genetics and medicine [86] . Ultimately the goal is the development of a highly biocompatible polymeric membrane reconciling the application specific benefits of both microcapsule and microcarrier systems to address clinical demands.…”

Section: Discussionmentioning

confidence: 99%

Living Cell Factories ‐ Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery

et al. 2015

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Minimally invasive delivery of "living cell factories" consisting of cells and therapeutic agents has gained wide attention for next generation biomaterial device systems for multiple applications including musculoskeletal tissue regeneration, diabetes and cancer. Cellularbased microcapsules and microcarrier systems offer several attractive features for this particular purpose. One such technology capable of generating these types of systems is electrohydrodynamic (EHD) spraying. Depending on various parameters including applied voltage, biomaterial properties (viscosity, conductivity) and needle geometry, complex structures and arrangements can be fabricated for therapeutic strategies. In this paper, we outline the advances in the use of EHD technology specifically in the manipulation of bioactive and dynamic material systems to control size, composition and configuration in the development of minimally invasive micro-scaled biopolymeric systems. The exciting therapeutic applications of this technology, future perspectives and associated challenges are also presented.

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“…The key feature of the microencapsulation technique is that cells are embedded in a semipermeable polymerized structure with the aim of protecting them from a host immune attack while allowing the diffusion of nutrients, oxygen, and metabolic products that ensure cell function and survival 18, 19. Primary human hepatocytes cultured on alginate microbeads in vitro for 3 days showed albumin and urea production.…”

Section: Implantable Technologies For Liver Therapiesmentioning

confidence: 99%

Liver tissue engineering: From implantable tissue to whole organ engineering

Mazza

Al‐Akkad

Rombouts

et al. 2017

Hepatology Communications

113

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The term “liver tissue engineering” summarizes one of the ultimate goals of modern biotechnology: the possibility of reproducing in total or in part the functions of the liver in order to treat acute or chronic liver disorders and, ultimately, create a fully functional organ to be transplanted or used as an extracorporeal device. All the technical approaches in the area of liver tissue engineering are based on allocating adult hepatocytes or stem cell‐derived hepatocyte‐like cells within a three‐dimensional structure able to ensure their survival and to maintain their functional phenotype. The hosting structure can be a construct in which hepatocytes are embedded in alginate and/or gelatin or are seeded in a pre‐arranged scaffold made with different types of biomaterials. According to a more advanced methodology termed three‐dimensional bioprinting, hepatocytes are mixed with a bio‐ink and the mixture is printed in different forms, such as tissue‐like layers or spheroids. In the last decade, efforts to engineer a cell microenvironment recapitulating the dynamic native extracellular matrix have become increasingly successful, leading to the hope of satisfying the clinical demand for tissue (or organ) repair and replacement within a reasonable timeframe. Indeed, the preclinical work performed in recent years has shown promising results, and the advancement in the biotechnology of bioreactors, ex vivo perfusion machines, and cell expansion systems associated with a better understanding of liver development and the extracellular matrix microenvironment will facilitate and expedite the translation to technical applications. (Hepatology Communications 2018;2:131–141)

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Cell encapsulation: Promise and progress

Cited by 551 publications

References 38 publications

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Living Cell Factories ‐ Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery

Liver tissue engineering: From implantable tissue to whole organ engineering

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