Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry

Shekdar, Kambiz; Langer, Jessica; Venkatachalan, Srinivasan P; Schmid, Lori; Anobile, Jonathan; Shah, Purvi K.; Lancaster, Amy; Babich, Olga; Dedova, Olga; Sawchuk, Dennis J.

doi:10.1007/s10529-021-03101-5

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Les « Molecular beacons », pour autant qu'ils soient fixés à l'aide de bras espaceurs suffisamment mobiles, vont pouvoir créer des complexes (concentration locale élevée) qui pourront être détruits par voie enzymatique (cf. par exemple Shekdar et al, 2021;Tyagi et Kramer, 1996 ;Täpp et al, 2000 ;Okamoto, 2011 ;Stallivieri et al, 2018)…”

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Photo-thérapie dynamique - entre modélisation et expérimentation - la rencontre des physiciens, chimistes, biologistes, oncologues, ingénieurs ou quand la modélisation diffusionnelle trouve ses limites...

Frochot¹,

André²

2022

Entropie

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1/2 where t is time and a and b are two experimental parameters. However, when we examine the behavior of synthesized D*BA couples, it is not possible to find the parameters a and b with a physical basis. Different hypotheses are proposed to try to understand the differences between modeling and experiments. An analysis of the points of view of the other disciplines involved in this approach to cancer treatment is also proposed.

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Section: T) (A)] Où (A) Est La Concentration De Aunclassified

Photo-thérapie dynamique - entre modélisation et expérimentation - la rencontre des physiciens, chimistes, biologistes, oncologues, ingénieurs ou quand la modélisation diffusionnelle trouve ses limites...

Frochot¹,

André²

2022

Entropie

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The stability of tastant detection by mouse lingual chemosensory tissue requires Regulator of G protein Signaling-21 (RGS21)

et al. 2021

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The T1R and T2R families of G protein-coupled receptors (GPCRs) initiate tastant perception by signaling via guanine nucleotide exchange and hydrolysis performed by associated heterotrimeric G proteins (Gαβγ). Heterotrimeric G protein signal termination is sped up by Gα-directed GTPase-accelerating proteins (GAPs) known as the Regulators of G protein Signaling (RGS proteins). Of this family, RGS21 is highly expressed in lingual epithelial cells and we have shown it acting in vitro to decrease the potency of bitterants on cultured cells. However, constitutive RGS21 loss in mice reduces organismal response to GPCR-mediated tastants - opposite to expectations arising from observed in vitro activity of RGS21 as a GAP and inhibitor of T2R signaling. Here, we show reduced quinine aversion and reduced sucrose preference by mice lacking RGS21 does not result from post-ingestive effects, as taste-salient brief-access tests confirm the reduced bitterant aversion and reduced sweetener preference seen using two-bottle choice testing. Eliminating Rgs21 expression after chemosensory system development, via tamoxifen-induced Cre recombination in eight week-old mice, led to a reduction in quinine aversive behavior that advanced over time, suggesting that RGS21 functions as a negative regulator to sustain stable bitter tastant reception. Consistent with this notion, we observed downregulation of multiple T2R proteins in the lingual tissue of Rgs21-deficient mice. Reduced tastant-mediated responses exhibited by mice lacking Rgs21 expression either since birth or in adulthood has highlighted the potential requirement for a GPCR GAP to maintain the full character of tastant signaling, likely at the level of mitigating receptor downregulation.

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Temporal patterns in taste sensitivity

Costanzo

2023

Nutrition Reviews

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Individuals vary in their ability to taste, and some individuals are more sensitive to certain tastes than others. Taste sensitivity is a predictor of various factors, such as diet, eating behavior, appetite regulation, and overall health. Furthermore, taste sensitivity can fluctuate within an individual over short to long periods of time: for example, in daily (diurnal) cycles, monthly (menstrual) cycles (in females), and yearly (seasonal) cycles. Understanding these temporal patterns is important for understanding individual eating habits and food preferences, particularly in the context of personalized and precision nutrition. This review provides a summary of the literature on taste sensitivity patterns across 3 temporal dimensions: daily, monthly, and yearly. Good evidence for diurnal patterns has been observed for sweet taste and fat taste, although the evidence is limited to rodent studies for the latter. Obese populations showed limited variation to sweet and fat taste sensitivities over a day, with limited variation in sweet taste sensitivity being linked to insulin resistance. There were mixed observations of temporal variation in sensitivity to sour and umami tastes, and there were no patterns in sensitivity to bitter taste. Menstrual patterns in sweet taste sensitivity were consistent with patterns in food intake. Other taste modality investigations had mixed findings that had little agreement across studies. Hormonal changes in females influence taste sensitivity to some degree, although the overall patterns are unclear. Seasonal patterns have been less well studied, but there is weak evidence that sweet, salty, and bitter taste sensitivities change across seasons. Differences in seasonal taste patterns have been observed in subgroups susceptible to mental health disorders, requiring further investigation. Patterns of taste sensitivity are evident across multiple temporal dimensions, and more research is needed to determine the influence of these patterns on food intake. Dysregulation of these patterns may also be a marker of certain diseases or health conditions, warranting further investigation. Notably, the alimentary tastes (umami, fat, and carbohydrate) are underrepresented in this research area and require additional investigation.

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Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry

Cited by 3 publications

References 21 publications

Photo-thérapie dynamique - entre modélisation et expérimentation - la rencontre des physiciens, chimistes, biologistes, oncologues, ingénieurs ou quand la modélisation diffusionnelle trouve ses limites...

Photo-thérapie dynamique - entre modélisation et expérimentation - la rencontre des physiciens, chimistes, biologistes, oncologues, ingénieurs ou quand la modélisation diffusionnelle trouve ses limites...

The stability of tastant detection by mouse lingual chemosensory tissue requires Regulator of G protein Signaling-21 (RGS21)

Temporal patterns in taste sensitivity

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