Cell Fate Decisions Within the Vascular Cambium–Initiating Wood and Bast Formation

Haas, A; Shi, Dongbo; Greb, Thomas

doi:10.3389/fpls.2022.864422

Cited by 10 publications

(6 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent genetic analyses of the model plant Arabidopsis thaliana led to the identification of the key regulators of the cambium (reviewed in Hoang et al. 2020 , Haas et al. 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms Underlying the Establishment and Maintenance of Vascular Stem Cells inArabidopsis thaliana

Shimadzu

Furuya

Kondo

2022

Plant and Cell Physiology

View full text Add to dashboard Cite

The vascular system plays pivotal roles in transporting water and nutrients throughout the plant body. Primary vasculature is established as a continuous strand, which subsequently initiates secondary growth through cell division. Key factors regulating primary and secondary vascular development have been identified in numerous studies, and the regulatory networks including these factors have been elucidated through omics-based approaches. However, the vascular system is composed of a variety of cells such as xylem and phloem cells, which are commonly generated from vascular stem cells. In addition, the vasculature is located deep inside the plant body, which makes it difficult to investigate the vascular development while distinguishing between vascular stem cells and developing xylem and phloem cells. Recent technical advances in tissue clearing method, RNA-seq analysis and tissue culture system overcome these problems by enabling the cell type-specific analysis during vascular development, especially with a special focus on stem cells. In this review, we summarize the recent findings on the establishment and maintenance of vascular stem cells.

show abstract

“…Recent genetic analyses of the model plant Arabidopsis thaliana led to the identification of the key regulators of the cambium (reviewed in Hoang et al. 2020 , Haas et al. 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms Underlying the Establishment and Maintenance of Vascular Stem Cells inArabidopsis thaliana

Shimadzu

Furuya

Kondo

2022

Plant and Cell Physiology

View full text Add to dashboard Cite

show abstract

“…Intriguingly, the xylanases affected cambial growth by specifically inhibiting xylem formation and increasing phloem formation, which was correlated with increased cytokinin content. Phloem differentiation and cambial cell division are known to be regulated by local maxima in cytokinins which in turn exclude auxin maxima by regulating distribution of PIN transporters inhibiting auxin-dependent activation of HD-ZIPIII transcription factors and xylem differentiation (Bishopp et al, 2011; Immanen et al, 2016; Haas et al, 2022). Moreover, we found three homologs of ZPR1 that is known to inactivate HD-ZIPIII transcription factors (Wenkel et al, 2007) upregulated in xylanase-expressing lines, which would provide additional mechanism suppressing xylem formation.…”

Section: Discussionmentioning

confidence: 99%

Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs

Sivan,

Urbancsok,

Donev

et al. 2024

Preprint

View full text Add to dashboard Cite

Wood of broad-leaf tree species is a valued source of renewable biomass for biorefinery and a target for genetic improvement efforts to reduce its recalcitrance. Glucuronoxylan (GX) plays a key role in recalcitrance through its interactions with cellulose and lignin. To reduce recalcitrance, we modified wood GX by expressing GH10 and GH11 endoxylanases from Aspergillus nidulans in hybrid aspen (Populus tremula L. x tremuloides Michx.) and targeting the enzymes to cell wall. The xylanases reduced tree height, modified cambial activity by increasing phloem and reducing xylem production, and reduced secondary wall deposition. Xylan molecular weight was decreased, and the spacing between acetyl and MeGlcA side chains was reduced in transgenic lines. The transgenic trees produced hypolignified xylem having thin secondary walls and deformed vessels. Glucose yields of enzymatic saccharification without pretreatment almost doubled indicating decreased recalcitrance. The transcriptomics, hormonomics and metabolomics data provided evidence for activation of cytokinin and ethylene signaling pathways, decrease in ABA levels, transcriptional suppression of lignification and a subset of secondary wall biosynthetic program, including xylan glucuronidation and acetylation machinery. Several candidate genes for perception of impairment in xylan integrity were detected. These candidates could provide a new target for uncoupling negative growth effects from reduced recalcitrance. In conclusion, our study supports the hypothesis that xylan modification generates intrinsic signals and evokes novel pathways regulating tree growth and secondary wall biosynthesis.

show abstract

“…This is in contrast to the important functional role of stem cells, such as xylem cambium, for adaptive robustness of organisms through cell renewal and de novo structuring [ 23 , 28 ]. Also, cambial vascular cells are important for stress perception and its transformation into energy-dependent cell fate decisions through cell cycle activity regulation [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analyses in Adult Olive Trees Indicate Acetaldehyde Release and Cyanide-Mediated Respiration Traits as Critical for Tolerance against Xylella fastidiosa and Suggest AOX Gene Family as Marker for Multiple-Resilience

Arnholdt-Schmitt,

Sircar,

Aziz

et al. 2024

Pathogens

View full text Add to dashboard Cite

Xylella fastidiosa (Xf) is a global bacterial threat for a diversity of plants, including olive trees. However, current understanding of host responses upon Xf-infection is limited to allow early disease prediction, diagnosis, and sustainable strategies for breeding on plant tolerance. Recently, we identified a major complex trait for early de novo programming, named CoV-MAC-TED, by comparing early transcriptome data during plant cell survival with SARS-CoV-2-infected human cells. This trait linked ROS/RNS balancing during first hours of stress perception with increased aerobic fermentation connected to alpha-tubulin-based cell restructuration and control of cell cycle progression. Furthermore, our group had advanced concepts and strategies for breeding on plant holobionts. Here, we studied tolerance against Xf-infection by applying a CoV-MAC-TED-related gene set to (1) progress proof-of-principles, (2) highlight the importance of individual host responses for knowledge gain, (3) benefit sustainable production of Xf-threatened olive, (4) stimulate new thinking on principle roles of secondary metabolite synthesis and microbiota for system equilibration and, (5) advance functional marker development for resilience prediction including tolerance to Xf-infections. We performed hypothesis-driven complex analyses in an open access transcriptome of primary target xylem tissues of naturally Xf-infected olive trees of the Xf-tolerant cv. Leccino and the Xf-susceptible cv. Ogliarola. The results indicated that cyanide-mediated equilibration of oxygen-dependent respiration and carbon-stress alleviation by the help of increased glycolysis-driven aerobic fermentation paths and phenolic metabolism associate to tolerance against Xf. Furthermore, enhanced alternative oxidase (AOX) transcript levels through transcription Gleichschaltung linked to quinic acid synthesis appeared as promising trait for functional marker development. Moreover, the results support the idea that fungal endophytes strengthen Xf-susceptible genotypes, which lack efficient AOX functionality. Overall, this proof-of-principles approach supports the idea that efficient regulation of the multi-functional AOX gene family can assist selection on multiple-resilience, which integrates Xf-tolerance, and stimulates future validation across diverse systems.

show abstract

Cell Fate Decisions Within the Vascular Cambium–Initiating Wood and Bast Formation

Cited by 10 publications

References 62 publications

Molecular Mechanisms Underlying the Establishment and Maintenance of Vascular Stem Cells inArabidopsis thaliana

Molecular Mechanisms Underlying the Establishment and Maintenance of Vascular Stem Cells inArabidopsis thaliana

Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs

Transcriptome Analyses in Adult Olive Trees Indicate Acetaldehyde Release and Cyanide-Mediated Respiration Traits as Critical for Tolerance against Xylella fastidiosa and Suggest AOX Gene Family as Marker for Multiple-Resilience

Contact Info

Product

Resources

About