Cell fate determination in the vertebrate retina.

Cepko, Constance L.; Austin, Christopher P.; Yang, Xian-Jie; Alexiades, Macrene; Ezzeddine, Diala

doi:10.1073/pnas.93.2.589

Cited by 895 publications

(758 citation statements)

References 55 publications

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“…The mammalian neural retina is composed of seven cell types, six neuronal and one glial, that differentiate from a common progenitor pool within defined temporal windows (Cepko et al, 1996;Livesey and Cepko, 2001). Proper spatial and temporal development of retinal neurons is attributed, in part, to the proper expression of proneural basic helix-loop-helix (bHLH) transcription factors in progenitors (Cepko, 1999).…”

Section: Introductionmentioning

confidence: 99%

Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

Hufnagel

Riesenberg

Saul

et al. 2007

Molecular and Cellular Neuroscience

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Retinal ganglion cell genesis requires the proneural bHLH transcription factor Math5 (Atoh7), but little is known about the regulatory elements that control its expression. Here, we investigate Math5 gene regulation using transgenic mice. These mice express GFP in the prenatal retina, livelabeling RGC axon migration and innervation of the brain. Unexpectedly, these Math5-GFP transgenes are also found in Math1 expression domains throughout the nervous system, intriguing since Math5 and Math1 normally exhibit nonoverlapping expression. Furthermore, Math5-GFP and Math1 are regulated similarly, by both Pax6 and Math1 itself, in the lower rhombic lip and dorsal spinal cord. We also show that Pax6 binds to particular Math5 and Math1 regulatory sequences in vitro. Together these data suggest that these atonal semi-orthologues may share conserved regulatory elements that are normally silent in the Math5 gene.

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Section: Introductionmentioning

confidence: 99%

Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

Hufnagel

Riesenberg

Saul

et al. 2007

Molecular and Cellular Neuroscience

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“…For example, in the retina, a part of the central nervous system, six types of neurons and one type of glial cells differentiate from common retinal precursor cells [1]. This differentiation proceeds according to the cell type-specific kinetics: some cell types such as ganglion cells differentiate only at early stages but others such as rod photoreceptors at later stages, probably responding to the different inductive cues in the environment.…”

Section: Introductionmentioning

confidence: 99%

The Notch-Hes pathway in mammalian neural development

1999

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A wide variety of neurons and glial cells differentiate from common precursor cells in the developing nervous system. During this process, Notch-mediated cell-cell interaction is essential for maintenance of dividing cells and subsequent generation of cell type diversity. Activation of Notch inhibits cellular differentiation, and abnormality of the Notch pathway leads to premature neuronal differentiation, the lack of some cell types, and severe defects of tissue morphogenesis. Recent data demonstrate that Notch fails to inhibit cellular differentiation in the absence of the bHLH genes Hes1 and Hes5, which functionally antagonize the neuronal bHLH genes such as Mash1. These results indicate that the two Hes genes are essential effectors for the Notch pathway and that neuronal differentiation is controlled by the pathway "Notch-Hes1/ Hes5-|Mash1".

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“…RPCs differentiate according to an ordered spatiotemporal pattern, largely conserved among vertebrates: ganglion cells are born first, followed by cone photoreceptors, horizontal and amacrine cells, whereas rod photoreceptors, bipolar and Müller cells are born last (Cepko et al, 1996;Stiemke and Hollyfield, 1995). Recent evidence supports the hypothesis that the commitment towards specific cell types occurs very early in RPCs (Lillien, 1998;Marquardt and Gruss, 2002;Marquardt et al, 2001).…”

Section: Introductionmentioning

confidence: 91%

The homeobox geneXbh1cooperates with proneural genes to specify ganglion cell fate within theXenopusneural retina

et al. 2004

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Recent studies on vertebrate eye development have focused on the molecular mechanisms of specification of different retinal cell types during development. Only a limited number of genes involved in this process has been identified. In Drosophila, BarH genes are necessary for the correct specification of R1/R6 eye photoreceptors. Vertebrate Bar homologues have been identified and are expressed in vertebrate retinal ganglion cells during differentiation; however, their retinal function has not yet been addressed. In this study, we report on the role of the Xenopus Bar homologue Xbh1 in retinal ganglion cell development and its interaction with the proneural genes Xath5 and Xath3, whose ability to promote ganglion cell fate has been demonstrated. We show that XHB1 plays a crucial role in retinal cell determination, acting as a switch towards ganglion cell fate. Detailed expression analysis, animal cap assays and in vivo lipofection assays, indicate that Xbh1 acts as a late transcriptional repressor downstream of the atonal genes Xath3 and Xath5. However, the action of Xbh1 on ganglion cell development is different and more specific than that of the Xath genes, and accounts for only a part of their activities during retinogenesis.

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Cell fate determination in the vertebrate retina.

Cited by 895 publications

References 55 publications

Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

The Notch-Hes pathway in mammalian neural development

The homeobox geneXbh1cooperates with proneural genes to specify ganglion cell fate within theXenopusneural retina

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