Cell-free chemoenzymatic starch synthesis from carbon dioxide

Cai, Tao; Sun, Haijie; Qiao, Jing; Zhu, Leilei; Zhang, Fan; Zhang, Jie; Tang, Zijing; Wei, Xinlei; Yang, Jiangang; Yuan, Qianqian; Wang, Wangyin; Yang, Xue; Chu, Huanyu; Wang, Qian; You, Chun; Ma, Hongwu; Sun, Yi; Li, Yin; Li, Can; Jiang, Huifeng; Wang, Qinhong; Ma, Yanhe

doi:10.1126/science.abh4049

Cited by 380 publications

(247 citation statements)

References 82 publications

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“…For example, it was recently shown that the photorespiratory metabolite 2PG acts as a feedback inhibitor of the Calvin cycle in Arabidopsis thaliana by inhibiting TPI and SBPase at sub-millimolar, biologically relevant concentrations (Flügel et al, 2017). An AMP-insensitive variant of the E. col FBPase was used to increase starch production from formate in a recently reported in vitro enzyme cascade (Cai et al, 2021). In the context of such works, LiP-SMap could aid in finding new regulators and guide protein engineering.…”

Section: Discussionmentioning

confidence: 99%

Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation

Sporre

Karlsen

Schriever

et al. 2022

Preprint

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Metabolite-level regulation of enzyme activity is important for coping with environmental shifts in bacteria. Improved understanding of such regulation could guide attempts to engineer more efficient strains for biochemical production. Recently developed proteomics methods allow for mapping of post-translational interactions, including metabolite-protein interactions, that may be relevant for quickly regulating pathway activity. While feed-back and feed-forward regulation in glycolysis has been investigated, there is relatively little study of metabolite-level regulation in the Calvin cycle, particularly in bacteria. Here, we applied limited proteolysis small molecule mapping (LiP-SMap) to identify and compare metabolite-protein interactions in four potential metabolic engineering host that fix CO2 using the Calvin cycle, including two photoautotrophs (cyanobacteria) and two chemoautotrophs. Species-specific interactions were observed, such as interactions with glucose-6-phosphate in the chemoautotroph Cupriavidus necator and interactions with glyoxylate in the cyanobacteria Synechocystis sp. PCC 6803, which suggests that metabolite-level regulation could be adapted to a certain metabolic capacity or lifestyle of these bacteria. Identified metabolite interactions with Calvin cycle enzymes fructose-1,6/sedoheptulose-1,7-bisphosphatase (F/SBPase) and transketolase were tested for effects on catalytic activity using kinetic assays. GAP increased the activity of both Synechocystis and Cupriavidus F/SBPase, which may act as a feed-forward activation mechanism in the Calvin cycle. A kinetic model incorporating regulations on F/SBPase generally enhanced flux control of ATP and NADPH supply over the cycle. We show that LiP-SMap is a promising technique to explore and uncover novel post-translational metabolic regulation, although the method could benefit from improved sensitivity and specificity.

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Section: Discussionmentioning

confidence: 99%

Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation

Sporre

Karlsen

Schriever

et al. 2022

Preprint

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“…In addition, synthetic biology is a new interdisciplinary field that uses engineering principles and biology knowledge to optimize or even de novo design metabolic pathways. Recently, a chemical-biochemical hybrid pathway for starch synthesis from carbon dioxide (CO 2 ) and hydrogen has been developed in a cell-free system through computational pathway design, modular assembly and substitution, and protein engineering (Cai et al, 2021b). Undoubtedly, development and application of metabolic engineering tools will pave the way for designing future crops with a superior CO 2 conversion rate, balanced nutrition, enriched beneficial metabolites, drugs or even vaccine.…”

Section: Perspectivementioning

confidence: 99%

Rice functional genomics: decades’ efforts and roads ahead

Chen

Deng

Ding

et al. 2021

Sci. China Life Sci.

146

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Rice (Oryza sativa L.) is one of the most important crops in the world. Since the completion of rice reference genome sequences, tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks. In this review, we summarize the research progress of rice biology over past decades, including omics, genome-wide association study, phytohormone action, nutrient use, biotic and abiotic responses, photoperiodic flowering, and reproductive development (fertility and sterility). For the roads ahead, cutting-edge technologies such as new genomics methods, high-throughput phenotyping platforms, precise genome-editing tools, environmental microbiome optimization, and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice, and facilitate integrations of the knowledge for agricultural applications.

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“…Thus, electrochemical combined with synthetic biotechnology spawned a new revolution for CO 2 fixation efficiency. By developing a chemoenzymatic system, CO 2 was first fixed through electrochemical to form methanol, coupling with multi-step enzymatic reaction, CO 2 was converted to starch at last with a rate ∼8.5-fold higher than starch synthesis in maize ( Cai et al, 2021 ). This is a typical case in fuse electrochemical synthetic biotechnology involvement, which gives attention to both efficiency and product quality.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering

Zhuang

Zhang

Xiao

et al. 2022

Front. Bioeng. Biotechnol.

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With the advancement of science, technology, and productivity, the rapid development of industrial production, transportation, and the exploitation of fossil fuels has gradually led to the accumulation of greenhouse gases and deterioration of global warming. Carbon neutrality is a balance between absorption and emissions achieved by minimizing carbon dioxide (CO2) emissions from human social productive activity through a series of initiatives, including energy substitution and energy efficiency improvement. Then CO2 was offset through forest carbon sequestration and captured at last. Therefore, efficiently reducing CO2 emissions and enhancing CO2 capture are a matter of great urgency. Because many species have the natural CO2 capture properties, more and more scientists focus their attention on developing the biological carbon sequestration technique and further combine with synthetic biotechnology and electricity. In this article, the advances of the synthetic biotechnology method for the most promising organisms were reviewed, such as cyanobacteria, Escherichia coli, and yeast, in which the metabolic pathways were reconstructed to enhance the efficiency of CO2 capture and product synthesis. Furthermore, the electrically driven microbial and enzyme engineering processes are also summarized, in which the critical role and principle of electricity in the process of CO2 capture are canvassed. This review provides detailed summary and analysis of CO2 capture through synthetic biotechnology, which also pave the way for implementing electrically driven combined strategies.

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Cell-free chemoenzymatic starch synthesis from carbon dioxide

Cited by 380 publications

References 82 publications

Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation

Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation

Rice functional genomics: decades’ efforts and roads ahead

Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering

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