Cell-free circulating miRNAs-lncRNAs-mRNAs as predictive markers for breast cancer risk assessment in women exposed to indoor air pollution

Agrawal, Deeksha; Kumari, Roshani; Ratre, Pooja; Rehman, Afreen; Srivastava, Rupesh Kumar; Reszka, Edyta; Goryacheva, Irina Yu.; Mishra, Pradyumna Kumar

doi:10.1016/j.cscee.2022.100267

Cited by 7 publications

(4 citation statements)

References 145 publications

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“…The interaction between lncRNA-miRNA-mRNA has been suggested to play a significant role in the onset of diseases, including BCs. Substantial investigations have shown that these RNAs’ regulatory networks can regulate the oncogenesis process . Considering this fact, we have developed fluorescent-based triple sensors for the detection of miRNAs, lncRNAs, and mRNAs collectively.…”

Section: Resultsmentioning

confidence: 99%

“…Substantial investigations have shown that these RNAs' regulatory networks can regulate the oncogenesis process. 4 Considering this fact, we have developed fluorescent-based triple sensors for the detection of miRNAs, lncRNAs, and mRNAs collectively. The existing methods for detection of these RNAs require additional labeling protocols, amplification techniques, and data normalization processes; however, the method discussed here is simple, specific, and has the ability to perform simultaneous detection of the three triad biomarkers (ccf-miRNA, lncRNAs, and mRNAs) without involving the additional complicated labeling protocols.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…It is now evident that the aberrant ceRNA network plays a vital role in the onset and progression of several noncommunicable diseases and malignancies, including breast cancer (BC), the primary source of cancer-related complications in women around the world. , As per recent estimates, one in every 20 women worldwide and one in eight women in countries with high incomes are affected by BC. Although the average mortality rate from BC has fallen due to the recent developments in therapeutic procedures, early detection and immediate treatment are two crucial factors for the cure of BC . As the metastatic disease causes more than 90% of BC-related deaths, numerous types of research have been performed to discover new and reliable biomarkers for BC, One of the recently developed biomarkers include ncRNAs such as lncRNAs, miRNAs, and mRNA, which circulates in the human body and has the potential to reflect the different steps of disease development and progression. , …”

Section: Introductionmentioning

confidence: 99%

“…Although the average mortality rate from BC has fallen due to the recent developments in therapeutic procedures, early detection and immediate treatment are two crucial factors for the cure of BC. 4 As the metastatic disease causes more than 90% of BC-related deaths, numerous types of research have been performed to discover new and reliable biomarkers for BC, One of the recently developed biomarkers include ncRNAs such as lncRNAs, miRNAs, and mRNA, which circulates in the human body and has the potential to reflect the different steps of disease development and progression. 5,6 These ncRNAs are interrelated and communicate in an intricate way to regulate the expression of vital genes.…”

Section: ■ Introductionmentioning

confidence: 99%

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Design and Fabrication of a Nanobiosensor for the Detection of Cell-Free Circulating miRNAS-LncRNAS-mRNAS Triad Grid

Ratre,

Nazeer,

Bhargava

et al. 2023

ACS Omega

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The increased understanding of the competitive endogenous RNA (ceRNA) network in the onset and development of breast cancers has suggested their use as promising disease biomarkers. Keeping these RNAs as molecular targets, we designed and developed an optical nanobiosensor for specific detection of the miRNAs-LncRNAs-mRNAs triad grid in circulation. The sensor was formulated using three quantum dots (QDs), i.e., QD-705, QD-525, and GQDs. These QDs were surface-activated and modified with a target-specific probe. The results suggested the significant ability of the developed nanobiosensor to identify target RNAs in both isolated and plasma samples. Apart from the higher specificity and applicability, the assessment of the detection limit showed that the sensor could detect the target up to 1 fg concentration. After appropriate validation, the developed nanobiosensor might prove beneficial to characterizing and detecting aberrant disease-specific cell-free circulating miRNAs-lncRNAs-mRNAs.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%