Cell free DNA as a component of forensic evidence recovered from touched surfaces

Quinones, Ignacio; Daniel, Barbara

doi:10.1016/j.fsigen.2011.01.004

Cited by 151 publications

(113 citation statements)

References 17 publications

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“…One obstacle that may occur during genotyping is heterozygous peak imbalance. Such heterologous peak imbalance was observed in 25% of lipstick and 16 % of blush stick samples, and these findings are consistent with early reports [8,19]. However, the heterozygous peak imbalance was not intense enough to prevent the interpretation of profiles.…”

Section: Discussionsupporting

confidence: 90%

“…These cosmetics contained a variety of dyes, waxes, and other constituent molecules which can influence the efficiency of the STR profiling. It is very difficult to attribute these impediments to any particular component of these cosmetics [19].…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have shown that approximately 0.4 million skin cells are shed by a person per day [5][6]. So when these cosmetics are applied onto the skin, these cells get attached onto the bars of lipstick and blush sticks.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Lipsticks and Blush Sticks in Genetic Profiling for Human Identification

Rasool

Ahmad

2016

AJFSFM

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ware version 1.0. The quantification results showed that the yield of DNA obtained from lipstick samples was greater than that of DNA obtained from blush stick samples. The real-time PCR results showed that only 16% of cosmetic samples had shown inhibition. The DNA profiles obtained from all blush stick samples were of good quality compared to those from lipstick samples. No profile was obtained from one blush stick sample (DNA 0.001 ng/µL) and four lipstick samples (DNA 0.001-0.003 ng/µL) because the amount of DNA in each of these samples was less than the amount required for successful amplification. DNA profiles were successfully generated from most of the samples of various available brands of lipsticks and blush sticks. This is the first study proving that DNA profiles can be generated from various lip and face cosmetics. AbstractThe core objectives of the current study are to generate human DNA profiles from used lipsticks and blush sticks of various brands available in Pakistan. A total of 12 international and local brands of lipsticks and blush sticks were selected. The lipsticks and blush sticks were applied by twenty different healthy female volunteers of 21-30 years of age. The heads of used lipsticks and blush sticks were swabbed with dry sterile cotton swabs. The qualitative and quantitative analysis was done by real time polymerase chain reaction (PCR), using a Quantifiler® Duo DNA Quantification Kit on Real Time PCR ABI™ 7500. Samples were amplified for 16 STR loci using an AmpFlSTR® Identifiler® PCR amplification kit on Thermocycler ABI 9700. The amplified product was run on Applied Biosystems 3130™ Genetic Analyzer. The genetic profiles were analyzed on GeneMapper® ID-X soft-

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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The Role of Lipsticks and Blush Sticks in Genetic Profiling for Human Identification

Rasool

Ahmad

2016

AJFSFM

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“…Scientists suggest that the mechanism could be similar to excretion of metabolites into sweat via sweat ducts. The DNA concentration in sweat has shown intraindividual and interindividual variation, indicating that various factors affect the DNA concentration deposited on the touched item [20].…”

Section: Discussionmentioning

confidence: 99%

“…It seems that trace DNA has several sources: corneocytes [1], anucleate keratinocytes which contain residual concentration of DNA and are daily sloughed off the skin's surface in large numbers and transferred from palms onto a touched surface by sweat [18], other types of nucleated cells [2,15,19], and sweat that contains cell-free DNA [3,20].…”

Section: Forensic Researchmentioning

confidence: 99%

Impact of Donor Age, Gender and Handling Time on the DNA Concentration Left on Different Surfaces

Gršković¹,

Markulin²,

Zidkova³

et al. 2014

Int J Biomed

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Background: We analyzed the correlation between several factors (donor age and gender, and handling time) and trace DNA concentration that participants left on different surfaces (paper, plastic, plastic coated metal) while holding items in their hands or rubbing them with their fingers, their palms, and the side of the palm of the dominant hand.Material and Methods: Sixty participants took part in the study. Items were swabbed with a moistened cotton swab. DNA was isolated using the Chelex procedure and quantified by real-time PCR.Results: We found that DNA concentration transferred to an item was independent of the handling time. On the contrary, it was dependent on the item's texture; the greatest concentration was left on plastic coated metal and the least on paper. The greatest concentration of trace DNA was left by participants from 35 to 44 years of age. Results of the study showed that men deposit a higher DNA concentration than do women.Conclusion: Item texture, donor age, and gender influence trace DNA concentration. Further investigations are necessary to fully understand the process of DNA transfer from donors to handled items.

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Transfer:DNA

Meakin

2016

Wiley Encyclopedia of Forensic Science

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Touch DNA , better referred to as trace DNA , can be transferred to a surface in a multitude of ways, both directly and indirectly. Although promising methods are on the horizon, it is currently not possible to reliably identify the biological source of trace DNA, be it from skin cells, other tissues or body fluids. This makes it particularly difficult for forensic experts to assess how trace DNA might have been transferred to the surface of interest. The detection of such transferred trace DNA is dependent on a plethora of factors, including those affecting its initial deposition, persistence, availability for onward transfer, and analytical recovery and availability for DNA profiling. Routine DNA profiling is now being conducted by extremely sensitive techniques making the recovery of trace DNA from a crime scene far more likely. As such, forensic experts must consider issues of DNA transfer, and any potential risks of contamination, when evaluating findings of trace DNA in casework.

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Cell free DNA as a component of forensic evidence recovered from touched surfaces

Cited by 151 publications

References 17 publications

The Role of Lipsticks and Blush Sticks in Genetic Profiling for Human Identification

The Role of Lipsticks and Blush Sticks in Genetic Profiling for Human Identification

Impact of Donor Age, Gender and Handling Time on the DNA Concentration Left on Different Surfaces

Transfer:DNA

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