Cell-free DNA as a potential marker to predict carbon tetrachloride-induced acute liver injury in rats

Gruenbaum, Benjamin F.; Boyko, Matthew; Delgado, Bertha; Douvdevany, Amos; Gruenbaum, Shaun E.; Melamed, Israel; Gideon, Micky; Cesnulis, Evaldas; Shapira, Yoram; Zlotnik, Alexander

doi:10.1007/s12072-012-9414-z

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…In a separate investigation, cfDNA levels were assessed in an animal model with induced liver injury, revealing elevated cfDNA levels alongside other specific biomarkers. Nonetheless, this study acknowledges that cfDNA elevation post-tissue damage may limit its clinical utility and impact on prognostic evaluations [77].…”

Section: Cell-free Dnamentioning

confidence: 94%

“…Extracellular DNA, or cfDNA, is the DNA molecule released, whole or fragmented, into body fluids of healthy subjects, such as blood, saliva, urine and/or feces [64,65], from a nucleated cell, and its level is usually much higher in people after physical effort [65]; however, in patients with various diseases such as sepsis, the inflammatory stress significantly increases many markers as well as the cell-free DNA level [56,[63][64][65][66][67][68][69]. Additionally, cf-DNA has been suggested as a potential predictive biomarker for several different conditions, including cancer (currently allows determination of a small fraction of tumor-derived) [70][71][72], stroke [73], trauma [74], transplantation (early detection of allograft rejection) [68,69,75,76], AKI [77,78], inflammatory and autoimmune diseases [79], and pregnancy (huge impact on prenatal medicine, where non-invasive prenatal testing is based on analysis of a fetal component of cfDNA in maternal blood) [80].…”

Section: Cell-free Dnamentioning

confidence: 99%

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The Role of Biomarkers in Diagnosis of Sepsis and Acute Kidney Injury

Ferreira,

Frota,

Gonzaga

et al. 2024

Biomedicines

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Sepsis and acute kidney injury (AKI) are two major public health concerns that contribute significantly to illness and death worldwide. Early diagnosis and prompt treatment are essential for achieving the best possible outcomes. To date, there are no specific clinical, imaging, or biochemical indicators available to diagnose sepsis, and diagnosis of AKI based on the KDIGO criterion has limitations. To improve the diagnostic process for sepsis and AKI, it is essential to continually evolve our understanding of these conditions. Delays in diagnosis and appropriate treatment can have serious consequences. Sepsis and AKI often occur together, and patients with kidney dysfunction are more prone to developing sepsis. Therefore, identifying potential biomarkers for both conditions is crucial. In this review, we talk about the main biomarkers that evolve the diagnostic of sepsis and AKI, namely neutrophil gelatinase-associated lipocalin (NGAL), proenkephalin (PENK), and cell-free DNA.

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Section: Cell-free Dnamentioning

confidence: 94%

Section: Cell-free Dnamentioning

confidence: 99%

The Role of Biomarkers in Diagnosis of Sepsis and Acute Kidney Injury

Ferreira,

Frota,

Gonzaga

et al. 2024

Biomedicines

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“…Interestingly, the characterization of cfDNA derived from the microbiome of non-human primates may allow the discovery and monitoring of taxa that cause zoonotic diseases [ 108 , 486 ]. Furthermore, various animal models have been used to study correlations between different pathological factors and corresponding changes in cfDNA, which may have implications in human biology and pathology, such as the study of traumatic brain injury in rats [ 487 ], carbon tetrachloride-induced acute liver injury in rats [ 488 ], perinatal asphyxia in piglets [ 489 ], tumor progression and metastases in rats [ 490 , 491 ], the onset of parturition in mice [ 492 ], and gateways regulating the release of tumor-derived cfDNA in mice [ 493 ].…”

Section: Characterization Of Cfdna In Animalsmentioning

confidence: 99%

New Perspectives on the Importance of Cell-Free DNA Biology

et al. 2022

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Body fluids are constantly replenished with a population of genetically diverse cell-free DNA (cfDNA) fragments, representing a vast reservoir of information reflecting real-time changes in the host and metagenome. As many body fluids can be collected non-invasively in a one-off and serial fashion, this reservoir can be tapped to develop assays for the diagnosis, prognosis, and monitoring of wide-ranging pathologies, such as solid tumors, fetal genetic abnormalities, rejected organ transplants, infections, and potentially many others. The translation of cfDNA research into useful clinical tests is gaining momentum, with recent progress being driven by rapidly evolving preanalytical and analytical procedures, integrated bioinformatics, and machine learning algorithms. Yet, despite these spectacular advances, cfDNA remains a very challenging analyte due to its immense heterogeneity and fluctuation in vivo. It is increasingly recognized that high-fidelity reconstruction of the information stored in cfDNA, and in turn the development of tests that are fit for clinical roll-out, requires a much deeper understanding of both the physico-chemical features of cfDNA and the biological, physiological, lifestyle, and environmental factors that modulate it. This is a daunting task, but with significant upsides. In this review we showed how expanded knowledge on cfDNA biology and faithful reverse-engineering of cfDNA samples promises to (i) augment the sensitivity and specificity of existing cfDNA assays; (ii) expand the repertoire of disease-specific cfDNA markers, thereby leading to the development of increasingly powerful assays; (iii) reshape personal molecular medicine; and (iv) have an unprecedented impact on genetics research.

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“…Those ecDNA molecules which circulate in the blood have been proposed as markers of various diseases. For instance, it has been suggested as a possible marker of hepatic fibrosis [ 124 ], nonalcoholic fatty acid liver disease [ 125 ] or as a valuable tool to predict carbon tetrachloride-induced liver injury in rats [ 126 ] and even progression toward hepatocellular carcinoma [ 127 ]. Contradictory evidence was provided by Blasi et al [ 128 ], who found higher levels of ecDNA in patients with acute-on-chronic liver failure compared to acute decompensation cirrhosis, although the amount of MPO-DNA complexes as the marker of NETs in the acute-on-chronic liver failure patients was not elevated.…”

Section: Citrullination Nets and Pads In Chronic Diseasesmentioning

confidence: 99%

The Citrullination-Neutrophil Extracellular Trap Axis in Chronic Diseases

Marônek

Gardlík²

2022

J Innate Immun

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Citrullination of proteins is crucial for the formation of neutrophil extracellular traps (NETs) – strands of nuclear DNA expulsed in the extracellular environment along with antimicrobial proteins in order to halt the spread of pathogens. Paradoxically, NETs may be immunogenic and contribute to inflammation. It is known that for the externalization of DNA, a group of enzymes called peptidyl arginine deiminases (PADs) is required. Current research often looks at citrullination, NET formation, PAD overexpression, and extracellular DNA (ecDNA) accumulation in chronic diseases as separate events. In contrast, we propose that citrullination can be viewed as the primary mechanism of autoimmunity, for instance by the formation of anti-citrullinated protein antibodies (ACPAs) but also as a process contributing to chronic inflammation. Therefore, citrullination could be at the center, connecting and impacting multiple inflammatory diseases in which ACPAs, NETs, or ecDNA have already been documented. In this review, we aimed to highlight the importance of citrullination in the etiopathogenesis of a number of chronic diseases and to explore the diagnostic, prognostic, and therapeutic potential of the citrullination-NET axis.

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Cell-free DNA as a potential marker to predict carbon tetrachloride-induced acute liver injury in rats

Abstract: CFD may be a useful biomarker for the prediction and measurement of ALI. There is no evidence to suggest that CFD is superior to other available noninvasive biomarkers.

Cited by 5 publications

References 40 publications

The Role of Biomarkers in Diagnosis of Sepsis and Acute Kidney Injury

The Role of Biomarkers in Diagnosis of Sepsis and Acute Kidney Injury

New Perspectives on the Importance of Cell-Free DNA Biology

The Citrullination-Neutrophil Extracellular Trap Axis in Chronic Diseases

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