Cell‐free DNA in amniotic fluid remains to be attached to HMGA2–implications for noninvasive prenatal diagnosis

Abstract

Our data clearly show that cells of the amniotic fluid strongly overexpress HMGA2 according to their fetal origin. The fact that apparently HMGA2 remains to be attached to cell-free DNA suggests interesting new approaches in noninvasive prenatal diagnosis.

Copy number variation sequencing‐based prenatal diagnosis using cell‐free fetal DNA in amniotic fluid

“…Although cffDNA is highly fragmented (100-400 bp), it represents the complete fetal genome and is essentially free of maternal or trophoblastderived DNA. [13][14][15] Several promising reports have demonstrated the feasibility of using cffDNA for array comparative genomic hybridization-based prenatal diagnosis. [16][17][18][19] To date, limited data exist regarding the accuracy and reproducibility of cffDNA as an alternative DNA template for molecular diagnosis.…”

“…In addition to amniocytes, amniotic fluid (AF) also contains cell‐free fetal DNA (cffDNA). Although cffDNA is highly fragmented (100–400 bp), it represents the complete fetal genome and is essentially free of maternal or trophoblast‐derived DNA . Several promising reports have demonstrated the feasibility of using cffDNA for array comparative genomic hybridization‐based prenatal diagnosis .…”

Tracking fetal development through molecular analysis of maternal biofluids

“…Winter et al found that high-mobility group protein HMGA2, which is primarily expressed by embryonic and fetal cells, is bound to cffDNA in amniotic fluid. This finding has significance for improving the yield of cffDNA from supernatant via immunoprecipitation techniques [80]. …”

QF‐PCR rapid aneuploidy screen and aCGH analysis of cell free fetal (cff) DNA in supernatant of compromised amniotic fluids (AF)

“…It has been shown that AF is a rich source of cff DNA . The supernatant of AF contains a high concentration of cff DNA (>17,000 GE/mL) and is a valuable source of pure fetal DNA free of maternal contamination or trophoblast derived nucleic acids . Although fragmented (~100–400 bp), cff DNA and RNA from AF supernatant represent the complete fetal genome, and several studies have shown its successful utilization for array comparative genomic hybridization (aCGH) analysis using bacterial artificial chromosome (BAC) arrays and transcriptomic analysis …”