Cell-free DNA Reveals Potential Zoonotic Reservoirs in Non-Human Primates

Kowarsky, Mark; Id, Vlaminck; Okamoto, Jennifer; Nf, Neff; LeBreton, Matthew; Nwobegabay, J; Tamoufé, Ubald; Jd, Ledoux; Tafon, B; Kiyang, John; Saylors, Karen; Nd, Wolfe

doi:10.1101/481093

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…Besides dogs, one study has measured cfDNA in cats with diffuse iris melanomas and concluded that cfDNA levels and integrity are not sufficient markers for diagnosis and prognosis [ 485 ]. Interestingly, the characterization of cfDNA derived from the microbiome of non-human primates may allow the discovery and monitoring of taxa that cause zoonotic diseases [ 108 , 486 ]. Furthermore, various animal models have been used to study correlations between different pathological factors and corresponding changes in cfDNA, which may have implications in human biology and pathology, such as the study of traumatic brain injury in rats [ 487 ], carbon tetrachloride-induced acute liver injury in rats [ 488 ], perinatal asphyxia in piglets [ 489 ], tumor progression and metastases in rats [ 490 , 491 ], the onset of parturition in mice [ 492 ], and gateways regulating the release of tumor-derived cfDNA in mice [ 493 ].…”

Section: Characterization Of Cfdna In Animalsmentioning

confidence: 99%

New Perspectives on the Importance of Cell-Free DNA Biology

et al. 2022

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Body fluids are constantly replenished with a population of genetically diverse cell-free DNA (cfDNA) fragments, representing a vast reservoir of information reflecting real-time changes in the host and metagenome. As many body fluids can be collected non-invasively in a one-off and serial fashion, this reservoir can be tapped to develop assays for the diagnosis, prognosis, and monitoring of wide-ranging pathologies, such as solid tumors, fetal genetic abnormalities, rejected organ transplants, infections, and potentially many others. The translation of cfDNA research into useful clinical tests is gaining momentum, with recent progress being driven by rapidly evolving preanalytical and analytical procedures, integrated bioinformatics, and machine learning algorithms. Yet, despite these spectacular advances, cfDNA remains a very challenging analyte due to its immense heterogeneity and fluctuation in vivo. It is increasingly recognized that high-fidelity reconstruction of the information stored in cfDNA, and in turn the development of tests that are fit for clinical roll-out, requires a much deeper understanding of both the physico-chemical features of cfDNA and the biological, physiological, lifestyle, and environmental factors that modulate it. This is a daunting task, but with significant upsides. In this review we showed how expanded knowledge on cfDNA biology and faithful reverse-engineering of cfDNA samples promises to (i) augment the sensitivity and specificity of existing cfDNA assays; (ii) expand the repertoire of disease-specific cfDNA markers, thereby leading to the development of increasingly powerful assays; (iii) reshape personal molecular medicine; and (iv) have an unprecedented impact on genetics research.

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Section: Characterization Of Cfdna In Animalsmentioning

confidence: 99%

New Perspectives on the Importance of Cell-Free DNA Biology

et al. 2022

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Applying the concept of liquid biopsy to monitor the microbial biodiversity of marine coastal ecosystems

Ferchiou

Caza

Boissel

et al. 2022

ISME Communications

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Liquid biopsy (LB) is a concept that is rapidly gaining ground in the biomedical field. Its concept is largely based on the detection of circulating cell-free DNA (ccfDNA) fragments that are mostly released as small fragments following cell death in various tissues. A small percentage of these fragments are from foreign (nonself) tissues or organisms. In the present work, we applied this concept to mussels, a sentinel species known for its high filtration capacity of seawater. We exploited the capacity of mussels to be used as natural filters to capture environmental DNA fragments of different origins to provide information on the biodiversity of marine coastal ecosystems. Our results showed that hemolymph of mussels contains DNA fragments that varied considerably in size, ranging from 1 to 5 kb. Shotgun sequencing revealed that a significant amount of DNA fragments had a nonself microbial origin. Among these, we found DNA fragments derived from bacteria, archaea, and viruses, including viruses known to infect a variety of hosts that commonly populate coastal marine ecosystems. Taken together, our study shows that the concept of LB applied to mussels provides a rich and yet unexplored source of knowledge regarding the microbial biodiversity of a marine coastal ecosystem.

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Cell-free DNA Reveals Potential Zoonotic Reservoirs in Non-Human Primates

Cited by 2 publications

References 51 publications

New Perspectives on the Importance of Cell-Free DNA Biology

New Perspectives on the Importance of Cell-Free DNA Biology

Applying the concept of liquid biopsy to monitor the microbial biodiversity of marine coastal ecosystems

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