Cell-free enzymatic L-alanine synthesis from green methanol

De Novo Multienzyme Synthetic Pathways for Lactic Acid Production

“…However, the product spectrum can be very limited, possibly due to (1) unfavorable growth conditions for the organisms in the presence of toxic intermediates; (2) incompatibility of the C1 assimilation pathway with the host central metabolic pathways; and (3) inefficient cofactor cycling . To circumvent these drawbacks, cell-free biomanufacturing is emerging for the direct monitoring and control of reaction processes and reduced accumulation of byproducts. , Many in vitro artificial C1 assimilation pathways have been successfully developed for the biosynthesis of sugars, , organic acids, − and amino acids. − Inspired by these achievements, we wondered whether other value-added chemical pathways could be designed for the cell-free assimilation of C1 compounds.…”

Modeling Enzymatic Cascade Reactions Immobilized in Plug‐Flow Reactors for Flow Biocatalysis

“…The use of immobilized enzymatic cascades in packed-bed reactors is of great interest due to its potential for enhanced space-time yields, high specificity, and sustainable production (less waste generation since less solvent and chemicals are required) of a variety of chemicals [5][6][7][8][9][10][11][12]. These multi-enzymatic pro-cesses, where several enzymes perform sequential reactions without the isolation and purification of intermediates, have experienced rapid growth in scientific and industrial applications [13][14][15][16][17][18][19][20][21][22][23][24][25][26].…”

Manipulating Activity and Chemoselectivity of a Benzaldehyde Lyase for Efficient Synthesis of α-Hydroxymethyl Ketones and One-Pot Enantio-Complementary Conversion to 1,2-Diols

“…α-Hydroxymethyl ketones are often encountered in the structure of many bioactive products − and are also routinely used as versatile building blocks for the manufacture of high-value important molecules in organic synthesis, with numerous applications as chiral auxiliaries, chiral ligands, or chiral catalysts for asymmetric synthesis (Scheme A). − Several cascade reactions have been established to obtain useful and high-value chemicals with α-hydroxymethyl ketone as an intermediate. − For example, dihydroxyacetone was an important α-hydroxymethyl ketone in the pathway for cell-free starch, hexoses, or l -alanine synthesis. − Owing to its great importance, the development of efficient methods for the synthesis of α-hydroxymethyl ketones is an important research focus in the pharmaceutical industry. The chemical methods for the preparation of α-hydroxymethyl ketones involve iodine-promoted activation, oxidation, and dioxygenation of alkenes, , substitution of α-haloketones, , oxidation of diols, decarboxylative oxidation of carboxylic acids, , and hydroxymethylation of aldehydes by N -heterocyclic carbenes (NHCs). , Compared to conventional chemical methods that often require high toxic and cost reagents, harsh reaction conditions, and sometimes low isolated yields, biocatalysis has become an attractive approach to prepare α-hydroxymethyl ketones.…”