Cell fusion phenomena detected after <i>in utero</i>
 transplantation of Ds-red-harboring porcine amniotic fluid stem cells into EGFP transgenic mice

Peng, Shu‐Fen; Chen, Yu-Hsu; Chou, C. James; Wang, Yao‐Horng; Lee, Hung-Maan; Cheng, Weijia; Shaw, Steven W.; Wu, Shinn-Chih

doi:10.1002/pd.4334

Cited by 5 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[4, 33, 34] Our laboratory also confirmed that using PMSCs in utero transplantation can result in a significant improvement in neurologic function at birth in the fetal rodent and ovine models of MMC. [3, 35–37] We confirmed that PMSCs did not persist after in utero transplantation. [3] Therefore, it is thought that the main regenerative effects of PMSCs arise from paracrine secretion mechanisms, including EVs such as exosomes and microvesicles.…”

Section: Discussionsupporting

confidence: 67%

Engineered neuron-targeting, placental mesenchymal stromal cell-derived extracellular vesicles for in utero treatment of myelomeningocele

Zhang

Chen

Gao

et al. 2021

Preprint

View full text Add to dashboard Cite

This study investigated the feasibility and efficiency of neuron-targeting hybrid placental mesenchymal stromal cell-derived extracellular vesicles (PMSC-EVs), engineered by membrane fusion with Targeted Axonal Import (TAxI) peptide modified, TrkB agonist 7,8-DHF-loaded liposomes for treatment of myelomeningocele (MMC) via intra-amniotic cavity administration. The prepared TAxI modified liposomes with 7,8-DHF were used to fuse with PMSC-EVs. Different fusion approaches were investigated and freeze-thaw-extrude method was found to be the optimal. The engineered PMSC-EVs had a uniform particle size and efficiently loaded 7,8-DHF. It also had typical markers of native EVs. Freeze-thaw-extrude process did not change the release profile of 7,8-DHF from engineered EVs compared to TAxI modified, 7,8-DHF loaded liposomes. The engineered EVs could elicit TrkB phosphorylation depending on the incorporation of 7,8-DHF while native EVs did not. The engineered EVs increased neurite outgrowth of apoptotic cortical neurons induced by staurosporine, suggesting that they exhibited neuroprotective function. In a rodent model of MMC, neuron-targeting, engineered EVs became an active targeting delivery system to MMC defect sites. Pups treated with engineered EVs had the lowest density of apoptotic cells and displayed a therapeutic outcome. The study suggests the potential use of engineered hybrid, active neuron-targeting EVs for the in utero treatment of MMC.

show abstract

Section: Discussionsupporting

confidence: 67%

Engineered neuron-targeting, placental mesenchymal stromal cell-derived extracellular vesicles for in utero treatment of myelomeningocele

Zhang

Chen

Gao

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…MSCs transduced via genetically modified viral vectors are considered important candidates for the treatment of prenatal diseases [42]; they do not easily form teratomas after transplantation, and they encompass a strong renewal ability [43]. In addition, MSCs can reside in recipients for a long time via cell fusion [44,45]. Furthermore, the enhanced fusion of MSCs with target cells is achieved with greater efficiency through the expression of vesicular stomatitis virus glycoprotein (VSVG), thereby facilitating a regenerative therapeutic outcome [46].…”

Section: Discussionmentioning

confidence: 99%

In Utero Cell Treatment of Hemophilia A Mice via Human Amniotic Fluid Mesenchymal Stromal Cell Engraftment

Kao,

Yen,

Fan

et al. 2023

IJMS

View full text Add to dashboard Cite

Hemophilia is a genetic disorder linked to the sex chromosomes, resulting in impaired blood clotting due to insufficient intrinsic coagulation factors. There are approximately one million individuals worldwide with hemophilia, with hemophilia A being the most prevalent form. The current treatment for hemophilia A involves the administration of clotting factor VIII (FVIII) through regular and costly injections, which only provide temporary relief and pose inconveniences to patients. In utero transplantation (IUT) is an innovative method for addressing genetic disorders, taking advantage of the underdeveloped immune system of the fetus. This allows mesenchymal stromal cells to play a role in fetal development and potentially correct genetic abnormalities. The objective of this study was to assess the potential recovery of coagulation disorders in FVIII knockout hemophilia A mice through the administration of human amniotic fluid mesenchymal stromal cells (hAFMSCs) via IUT at the D14.5 fetal stage. The findings revealed that the transplanted human cells exhibited fusion with the recipient liver, with a ratio of approximately one human cell per 10,000 mouse cells and produced human FVIII protein in the livers of IUT-treated mice. Hemophilia A pups born to IUT recipients demonstrated substantial improvement in their coagulation issues from birth throughout the growth period of up to 12 weeks of age. Moreover, FVIII activity reached its peak at 6 weeks of age, while the levels of FVIII inhibitors remained relatively low during the 12-week testing period in mice with hemophilia. In conclusion, the results indicated that prenatal intrahepatic therapy using hAFMSCs has the potential to improve clotting issues in FVIII knockout mice, suggesting it as a potential clinical treatment for individuals with hemophilia A.

show abstract

“…E27 porcine mesencephalic tissue was dissected from either embryonic Lanyu pig group ( n = 6) (Taitung Animal Propagation Station, Livestock Research Institute, Taitung, Taiwan) (12,30,72) or transgenic pigs harboring the RFP group ( n = 6) (49,53) transplanted in the lesioned striatum of PD rats. The dissection areas were selected according to the protocols of Galpern et al (21) and Larsson et al (40), with minor modifications as follows.…”

Section: Methodsmentioning

confidence: 99%

PET Imaging of Serotonin Transporters with 4-[¹⁸F]-ADAM in a Parkinsonian Rat Model with Porcine Neural Xenografts

Chiu

Weng

et al. 2016

Cell Transplant

Self Cite

View full text Add to dashboard Cite

Parkinson's disease (PD) is a neurodegenerative disease characterized by a loss of dopaminergic neurons in the nigrostriatal pathway. Apart from effective strategies to halt the underlying neuronal degeneration, cell replacement now offers novel prospects for PD therapy. Porcine embryonic neural tissue has been considered an alternative source to human fetal grafts in neurodegenerative disorders because its use avoids major practical and ethical issues. This study was undertaken to evaluate the effects of embryonic day 27 (E27) porcine mesencephalic tissue transplantation in a PD rat model using animal positron emission tomography (PET) coupled with 4-[ 18 F]-ADAM, a serotonin transporter (SERT) imaging agent. The parkinsonian rat was induced by injecting 6-hydroxydopamine into the medial forebrain bundle (MFB) of the right nigrostriatal pathway. The apomorphine-induced rotation behavioral test and 4-[ 18 F]-ADAM/animal PET scanning were carried out following 6-OHDA lesioning. At the second week following 6-OHDA lesioning, the parkinsonian rat rotates substantially on apomorphine-induced contralateral turning. In addition, the mean striatal-specific uptake ratio (SUR) of 4-[ 18 F]-ADAM decreased by 44%. After transplantation, the number of drug-induced rotations decreased markedly, and the mean SUR of 4-[ 18 F]-ADAM and the level of SERT immunoreactivity (SERT-ir) in striatum were partially restored. The mean SUR level was restored to 71% compared to that for the contralateral intact side, which together with the abundant survival of tyrosine hydroxylase (TH) neurons accounted for functional recovery at the fourth week postgraft. In regard to the extent of donor-derived cells, we found the neurons of the xenografts from E27 transgenic pigs harboring red fluorescent protein (RFP) localized with TH-ir cells and SERT-ir in the grafted area. Thus, transplanted E27 porcine mesencephalic tissue may restore dopaminergic and serotonergic systems in the parkinsonian rat. The 4-[ 18 F]-ADAM/animal PET can be used to detect serotonergic neuron loss in PD and monitor the efficacy of therapy.

show abstract

Cell fusion phenomena detected after in utero transplantation of Ds-red-harboring porcine amniotic fluid stem cells into EGFP transgenic mice

Abstract: In utero xenotransplantation of pAFSCs fused with recipient intestinal cells instead of differentiating or maintaining the undifferentiated status in the tissue.

Cited by 5 publications

References 30 publications

Engineered neuron-targeting, placental mesenchymal stromal cell-derived extracellular vesicles for in utero treatment of myelomeningocele

Engineered neuron-targeting, placental mesenchymal stromal cell-derived extracellular vesicles for in utero treatment of myelomeningocele

In Utero Cell Treatment of Hemophilia A Mice via Human Amniotic Fluid Mesenchymal Stromal Cell Engraftment

PET Imaging of Serotonin Transporters with 4-[¹⁸F]-ADAM in a Parkinsonian Rat Model with Porcine Neural Xenografts

Contact Info

Product

Resources

About

Cell fusion phenomena detected after in utero transplantation of Ds-red-harboring porcine amniotic fluid stem cells into EGFP transgenic mice

Abstract: In utero xenotransplantation of pAFSCs fused with recipient intestinal cells instead of differentiating or maintaining the undifferentiated status in the tissue.

Cited by 5 publications

References 30 publications

Engineered neuron-targeting, placental mesenchymal stromal cell-derived extracellular vesicles for in utero treatment of myelomeningocele

Engineered neuron-targeting, placental mesenchymal stromal cell-derived extracellular vesicles for in utero treatment of myelomeningocele

In Utero Cell Treatment of Hemophilia A Mice via Human Amniotic Fluid Mesenchymal Stromal Cell Engraftment

PET Imaging of Serotonin Transporters with 4-[18F]-ADAM in a Parkinsonian Rat Model with Porcine Neural Xenografts

Contact Info

Product

Resources

About

PET Imaging of Serotonin Transporters with 4-[¹⁸F]-ADAM in a Parkinsonian Rat Model with Porcine Neural Xenografts