Cell Growth Characteristics and Differentiation Frequency of Adherent Equine Bone Marrow–Derived Mesenchymal Stromal Cells: Adipogenic and Osteogenic Capacity

Vidal, Martin A.; Kilroy, Gail; Johnson, Jill; Lopez, Mandi J.; Moore, Rustin M.; Gimble, Jeffrey M.

doi:10.1111/j.1532-950x.2006.00197.x

Cited by 183 publications

(206 citation statements)

References 49 publications

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“…The cell population with the fusiform appearance described here in samples from both adipose tissue and bone marrow were morphologically similar to mesenchymal stem cells from human (Zuk et al 2001, You et al 2009), sheep (Fadel et al 2011), horse (Fortier et al 1998, Smith et al 2003, Hegewald et al 2004, Vidal et al 2006, 2007, Maia et. al 2012, De Vita et al 2013, and other animals reported in many studies, in which these cells were described as star-shaped or fibroblast-like.…”

Section: Discussionmentioning

confidence: 77%

Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

2013

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Pesq. Vet. Bras. 33(Supl.1):20-24, dezembro 2013 20 RESUMO.-[Cultivo de células da fração mononuclear da medulla óssea e da fração vascular stromal do tecido adipose de equinos em diferentes meios.] O objetivo deste estudo foi avaliar o cultivo de células da fração mononuclear da medula óssea e da fração vascular estromal do tecido adiposo de equinos em dois diferentes meios. Cinco cavalos foram submetidos à aspiração da medula óssea do esterno e à coleta de tecido adiposo da região glútea, pró-xima à inserção da cauda. A fração mononuclear e a fração vascular estromal foram obtidas das amostras e cultivadas em meio DMEM suplementado com soro fetal bovino a 10% ou em meio AIM-V. As culturas foram observadas uma vez por semana com um microscópio de luz invertida, com o intuito de se realizar uma análise qualitativa das características morfológicas das células, bem como do aspecto geral do cultivo celular. As unidades formadoras de colônia (CFU) foram contadas nos dias 5, 15 e 25 do cultivo celular. Durante a primeira semana, foram observadas diferenças entre amostras obtidas de mesma origem mantidas em diferentes meios. O número de colônias foi significativamente maior nas amostras de medula óssea em relação às amostras de tecido adiposo.TERMOS DE INDEXAÇÃO: Cavalos, medula óssea, tecido adiposo, meios de cultura. INTRODUCTIONThe bone marrow aspirate concentrate (BMAC) has been used to induce bone formation in humans with osseous defects, nonunion, or osteonecrosis. In horses BMAC has been used to improve cartilage, bone, and tendon repair, The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.

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Section: Discussionmentioning

confidence: 77%

Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

2013

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“…The isolation procedure for MSCs continues to be based on their plastic adherence properties [7,[25][26][27][28]. In addition, the nomenclature used to distinguish between isolated cells types is lacking.…”

Section: Discussionmentioning

confidence: 99%

The CD271 expression could be alone for establisher phenotypic marker in Bone Marrow derived mesenchymal stem cells.

Flores-Torales

Orozco-Barocio²,

González-Ramella³

et al. 2011

Folia Histochem Cytobiol.

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“…The most characterized sources of MSCs are bone marrow (Fortier et al 1998, Ringe et al 2002, Mitchell et al 2003, Smith et al 2003, Kern et al 2006, Vidal et al 2006, Arnhold et al 2007, Vidal et al 2008) and the adipose tissue (Kern et al 2006, Vidal et al 2008; however, the procedures employed to isolate these tissues are still invasive and cells are usually obtained with low efficiency. Furthermore, their differentiative and proliferative potential has been reported to decrease with the age of the donor (Pittenger et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Corradetti¹,

Meucci²,

Bizzaro³

et al. 2013

Reproduction

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Amnion and amniotic fluid (AF) are noncontroversial and inexhaustible sources of mesenchymal stem cells (MSCs) that can be harvested noninvasively at low cost. As in humans, also in veterinary field, presumptive stem cells derived from these tissues reveal as promising candidates for disease treatment, specifically for their plasticity, their reduced immunogenicity, and high anti-inflammatory potential. The aim of this work is to obtain and characterize, for the first time in bovine species, presumptive MSCs from the epithelial portion of the amnion (AECs) and from the AF (AF-MSCs) to be used for clinical applications. AECs display a polygonal morphology, whereas AF-MSCs exhibit a fibroblastic-like morphology only starting from the second passage, being heterogeneous during the primary culture. For both lines, the proliferative ability has been found constant over the ten passages studied and AECs show a statistically lower (P!0.05) doubling time with respect to AF-MSCs. AECs express MSC-specific markers (ITGB1 (CD29), CD44, ALCAM (CD166), ENG (CD105), and NT5E (CD73)) from P1 to P3; in AF-MSCs, only ITGB1, CD44, and ALCAM mRNAs are detected; NT5E is expressed from P2 and ENG has not been found at any passage. AF-MSCs and AECs are positive for the pluripotent markers (POU5F1 (OCT4) and MYC (c-Myc)) and lack of the hematopoietic markers. When appropriately induced, both cell lines are capable of differentiating into ectodermal and mesodermal lineages. This study contributes to reinforce the emerging importance of these cells as ideal tools in veterinary medicine. A deeper evaluation of the immunological properties needs to be performed in order to better understand their role in cellular therapy.

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Cell Growth Characteristics and Differentiation Frequency of Adherent Equine Bone Marrow–Derived Mesenchymal Stromal Cells: Adipogenic and Osteogenic Capacity

Abstract: The results have direct relevance to the use of BM as a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.

Cited by 183 publications

References 49 publications

Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

The CD271 expression could be alone for establisher phenotypic marker in Bone Marrow derived mesenchymal stem cells.

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

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