Cell hydrophobicity is a triggering force of biogranulation

Liu, Yu; Yang, Shu-Fang; Tay, Joo‐Hwa; Liu, Qi-Shan; Qin, Lei; Li, Yong

doi:10.1016/j.enzmictec.2003.12.009

Cited by 142 publications

(45 citation statements)

References 71 publications

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“…Previous studies indicated that there is a strong correlation between microbial auto-aggregation and cell surface hydrophobicity (Baldi et al, 1999;Del Re et al, 2000;Kos et al, 2003;Liu et al, 2003bLiu et al, , 2004aTay et al, 2000). Improved hydrophobicity of bacteria is conductive to creating a favorable environment for the contact of bacterium-to-bacterium or impelling bacteria out of aqueous phase environments to aggregate.…”

Section: Triggering Forces For Microbial Granulationmentioning

confidence: 95%

Rapid formation of hydrogen‐producing granules in an anaerobic continuous stirred tank reactor induced by acid incubation

Zhang

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Tay

et al. 2006

Biotech & Bioengineering

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A novel approach to rapidly initiate granulation of hydrogen-producing sludge was developed in an anaerobic continuous stirred tank reactor at 37 degrees C. To induce microbial granulation, the acclimated culture was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The culture was resumed to pH 5.5 after the incubation and the reactor was operated at hydraulic retention times (HRTs) of 12, 6, 2, 1, and 0.5 h in sequence. Microbial aggregation took place immediately with the initiation of acid incubation and granules were developed at 114 h. No granule was observed in the absence of acid incubation in the control test. Changing the culture pH resulted in improvement in surface physicochemical properties of the culture favoring microbial granulation. The zeta potential increased from -11.6 to -3.5 mV, hydrophobicity in terms of contact angle improved from 31 degrees to 43 degrees and extracellular proteins/polysaccharides ratio increased from 0.2 to 0.5-0.8. Formation of granular sludge facilitated biomass retention of up to 32.2 g-VSS/L and enhanced hydrogen production. The hydrogen production rate and hydrogen yield increased with the reduction in HRT at an influent glucose concentration of 10 g/L once steady granular sludge layer was formed, achieving the respective peaks of 3.20 L/L x h and 1.81 mol-H(2)/mol-glucose at 0.5 h HRT. The experimental results suggested that acid incubation was able to initiate the rapid formation of hydrogen-producing granules by regulating the surface characteristics of microbial aggregates in a well-mixed reactor, which enhanced the hydrogen production.

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Section: Triggering Forces For Microbial Granulationmentioning

confidence: 95%

Rapid formation of hydrogen‐producing granules in an anaerobic continuous stirred tank reactor induced by acid incubation

Zhang

Show

Tay

et al. 2006

Biotech & Bioengineering

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“…Many aggregate-forming bacteria have been reported to possess a high cell surface hydrophobicity (Liu et al 2004), which is considered to facilitate the cells adherence to hydrocarbons. Therefore, the cell surface hydrophobicity of the aggregates and free-living cells of the tank-2 consortium was determined by the BATH assay.…”

Section: Characteristics Of Bacterial Aggregates Formed By the Tank-2mentioning

confidence: 99%

Establishment and characterization of turbine oil-degrading bacterial consortia

Hosokawa

Sakaguchi

Okuyama

2010

International Biodeterioration & Biodegradation

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A microbial consortium capable of degrading turbine oil (TuO) consisting mainly of recalcitrant branched and polycyclic alkanes, has been established using a sludge sample collected from an oil-water separation tank of a hydraulic plant, by repeated enrichment cultivation. When this consortium, named the tank-2 consortium, was cultivated in a minimal salts medium containing 0.5% TuO, it degraded approximately 90% of TuO at 30 °C under shaking at 160 rpm in five days. Dur ing cultivation, the consor tium for med cell aggregates. Four teen bacter ial species were isolated from the consortium. Although the mixtures of all of the isolated str ains (IM) and predominant str ains selected from the IM (PM) degr aded approximately 60% of the TuO maximally in seven days after repeated enr ichment, the degradation r ate was not maintained. The decreased r ate of TuO degradation in the PM was recovered by inoculation with a mixture (AM) consisting of isolates involved in the cell aggregate for mation in the 3 tank-2 consor tium, suggesting that the for mation of bacter ial aggregates is an important factor for the degradation of TuO by the bacter ial consortia. Interestingly, the for mation of bacter ial aggregates was only obser ved when cyclic alkanes were added to the consortium as a source of car bon. The cell sur face hydrophobicity of the bacter ial aggregates was significantly high (77.6% ). These results suggest that the growth of bacter ia with high cell hydrophobicity can be induced by the cyclic alkanes fr action of TuO, and that the for mation of aggregates facilitates cells to for m contact with the recalcitr ant components of TuO and uptakes these components.

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“…Several studies have suggested that bacterial attachment can be influenced by the physicochemical properties of the attachment surface and the attaching bacteria (13,14). To investigate whether the strong attachment of the Salmonella strain to BC composites was influenced by these factors, assays of the attachment of the bacterial pair to abiotic surfaces with different hydrophobicities and other physicochemical properties were carried out.…”

Section: Chemical Composition Analysismentioning

confidence: 99%

“…Other studies have suggested that greater bacterial hydrophobicity favors bacterial adhesion to most surfaces (11,12). Increased hydrophobicity also favors cell-to-cell adhesion, which leads to greater autoaggregation (13,14). Similarly, coaggregation has been shown to be dependent on autoaggregation (14).…”

mentioning

confidence: 99%

Pectin and Xyloglucan Influence the Attachment of Salmonella enterica and Listeria monocytogenes to Bacterial Cellulose-Derived Plant Cell Wall Models

Tan

Rahman

Dykes

2016

Appl Environ Microbiol

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bMinimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. Using bacterial cellulose-derived plant cell wall models, we showed that the presence of pectin alone or xyloglucan alone affected the attachment of three Salmonella enterica strains (Salmonella enterica subsp. enterica serovar Enteritidis ATCC 13076, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028, and Salmonella enterica subsp. indica M4) and Listeria monocytogenes ATCC 7644. In addition, we showed that this effect was modulated in the presence of both polysaccharides. Assays using pairwise combinations of S. Typhimurium ATCC 14028 and L. monocytogenes ATCC 7644 showed that bacterial attachment to all plant cell wall models was dependent on the characteristics of the individual bacterial strains and was not directly proportional to the initial concentration of the bacterial inoculum. This work showed that bacterial attachment was not determined directly by the plant cell wall model or bacterial physicochemical properties. We suggest that attachment of the Salmonella strains may be influenced by the effects of these polysaccharides on physical and structural properties of the plant cell wall model. Our findings improve the understanding of how Salmonella enterica and Listeria monocytogenes attach to plant cell walls, which may facilitate the development of better ways to prevent the attachment of these pathogens to such surfaces.A ccording to the World Health Organization (WHO), almost 1.5 million cases of human salmonellosis are reported globally every year (1-3). Fresh produce is an important vehicle for the transmission of human pathogens and a major source of foodborne microbial outbreaks worldwide. While Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium were historically linked to the majority of the outbreaks from food of animal origin, a number of Salmonella serovars have now been strongly linked to fresh produce (4-6).Pathogenic bacteria associated with fresh produce may establish themselves on plant surfaces and cause disease; therefore, the initial process of bacterial attachment is a crucial step in their transmission (7,8). A study by Saggers et al. (9) suggested that plant cell wall (PCW) components at the PCW junction, particularly pectin, may provide receptor sites for bacterial attachment. In addition to the structural components of the PCW, the physicochemical properties (such as hydrophobicity and charge) of both the attachment surface and the attaching bacteria influence bacterial adhesion. For example, it has been suggested that bacteria that exhibit greater surface hydrophobicity than other strains at...

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Cell hydrophobicity is a triggering force of biogranulation

Cited by 142 publications

References 71 publications

Rapid formation of hydrogen‐producing granules in an anaerobic continuous stirred tank reactor induced by acid incubation

Rapid formation of hydrogen‐producing granules in an anaerobic continuous stirred tank reactor induced by acid incubation

Establishment and characterization of turbine oil-degrading bacterial consortia

Pectin and Xyloglucan Influence the Attachment of Salmonella enterica and Listeria monocytogenes to Bacterial Cellulose-Derived Plant Cell Wall Models

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