Cell Immobilization

Tanaka, Atsuo; Kawamoto, Takuo

doi:10.1002/0471250589.ebt041

Cited by 5 publications

(11 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since each method has its own advantages and drawbacks, the selection of suitable immobilisation method depends on the enzyme (different properties of various β-galactosidases, such as molecule weight, protein chain length, and position of the active site), matrix, reaction conditions, reactor, etc. (Tanaka & Kawamoto 1999).…”

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

“…Compare to other techniques this method has the following advantages: enzymes does not leak or detach from the carrier, the biocatalyst can easily interact with the substrate, since being on the surface of the carrier. On the other hand, the major disadvantages are high costs and low activity yield owing to exposure of the biocatalyst to toxic reagents or severe reaction conditions (Tanaka & Kawamoto 1999). There were several matrices used for β-galactosidase immobilisation.…”

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

“…Next disadvantages of the method are diffusion limitations. Entrapment method is classified into five major types: lattice, microcapsule, liposome, membrane, and reverse micelle (Tanaka & Kawamoto 1999).…”

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

“…Immobilisation by adsorption is based on the physical interactions between the biocatalyst and the carrier, such as hydrogen bonding, hydrophobic interactions, van der Waals force, and their combinations. Despite its simplicity, this immobilisation method is significantly limited by the tendency of enzyme to desorb from the support and sensitivity to environmental conditions, such as temperature and iones concentration (Tanaka & Kawamoto 1999). The immobilised β-galactosidase particles (size 1-2 mm) prepared by physical adsorption of the enzyme on the porous ceramic support and intermolecular cross-linking with glutaraldehyde reached binding efficiency 80% and good operational stability.…”

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

See 3 more Smart Citations

Perspectives and applications of immobilised β-galactosidase in food industry - a review

Grosová¹,

Rosenberg²,

Rebroš³

2008

Czech J. Food Sci.

119

View full text Add to dashboard Cite

Grosová Z., Rosenberg M., Rebroš M. (2008): Perspectives and applications of immobilised β-galactosidase in food industry -a review. Czech J. Food Sci., 26: 1-14.β-Galactosidase is an important industrial enzyme in the hydrolysis of milk and whey lactose. The enzymatic hydrolysis of lactose allows to avoid health and environmental problems posed by this disaccharide. In addition, this enzyme catalyses the formation of galacto-oligosaccharides, which are prebiotic additives for the so-called "healthy foods". β-Galactosidase is one of the relatively few enzymes that have been used in large-scale processes in both free and immobilised forms. This article presents a review of recent trends in immobilisation of β-galactosidase and their application in food industry.

show abstract

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

Section: Immobilisation Of β-Galactosidasementioning

confidence: 99%

See 2 more Smart Citations

Perspectives and applications of immobilised β-galactosidase in food industry - a review

Grosová¹,

Rosenberg²,

Rebroš³

2008

Czech J. Food Sci.

119

View full text Add to dashboard Cite

show abstract

“…Immobilization is done by the four different methods: carrier-binding, cross-linking, entrapping and combination of other three [8]. Alginate, carrageenan, polyacrylamide and glass beads are commonly used as a carrier for the cells [9,10].…”

Section: Introductionmentioning

confidence: 99%

Development of Methods for Immobilization and Usages of Immobilized Recombinant Bioluminescent Strain, Pseudomonas Putida Mt-2 KG1206, Stored by Deep-Freezing

Kim¹,

Ic²

2015

J Biotechnol Biomater

View full text Add to dashboard Cite

Deep-freezing process can be used practically for environmental monitoring. However, for the organism to be useful in environmental applications, the strain of bacteria exhibits a high intensity of activity following reconstitution after deep-freezing. An overall protocol for immobilization and reconstitution of recombinant bioluminescent bacteria, preserved by deep-freezing was investigated for future biomonitoring. Tested strain KG1206 has ability to produce bioluminescence in the presence of toluene analogs and those intermediates. Immobilization using glass beads with subcultured cells showed better results than other conditions, such as alginate beads or centrifuged cell pellets. Beads number, thawing time, and amount of reconstitution solution influence on bioluminescence activity. Among tested conditions, followings were appeared as optimum conditions: working volume 15 mL of serum vials, approximately 20-30 glass beads per vial, 3 h thawing time, and 2-5 mL reconstitution solution. Potassium nitrate (KNO 3 ) greatly stimulates the low bioluminescence activity of immobilized strain, preserved by deep freezing, in the range of 2.5 to 21 times of untreated one.Keywords: Bioluminescence; Biomonitoring; Deep-freezing; Immobilization; Recombinant Significance and Impact of the StudyThis study indicated that type of immobilization matrix, cell conditions (subcultured or centrifuged pellets), matrix (beads) numbers, volume of reconstitution solution, and thawing time are important factors on bioluminescence activity of reconstituted culture, preserved by deep freezing. Overall, this investigation demonstrated the ability of immobilized bacteria on glass beads, preserved by deep freezing, for the monitoring of a specific group of environmental pollutants using a stimulant KNO 3 , suggesting the potential method for preliminary application in a field-ready portable bioassay.

show abstract