Cell immobilization for microbial production of 1,3-propanediol

Abstract

Cell and enzyme immobilization are often used for industrial production of high-value products. In recent years, immobilization techniques have been applied to the production of value-added chemicals such as 1,3-Propanediol (1,3-PDO). Biotechnological fermentation is an attractive alternative to current 1,3-PDO production methods, which are primarily thermochemical processes, as it generates high volumetric yields of 1,3-PDO, is a much less energy intensive process, and generates lower amounts of environmental organic pollutants. Although several approaches including: batch, fed-batch, continuous-feed and two-step continuous-feed were tested in suspended systems, it has been well demonstrated that cell immobilization techniques can significantly enhance 1,3-PDO production and allow robust continuous production in smaller bioreactors. This review covers various immobilization methods and their application for 1,3-PDO production.

Deletingpckimproves growth and suppresses by-product formation during 1,3-propanediol fermentation byKlebsiella pneumoniae

“…; Gungormusler‐Yilmaz et al . ; Johnson and Rehmann ). In addition to its highly efficient 1,3‐PD production, Kl.…”

“…; Gungormusler‐Yilmaz et al . ), and it mainly begins with a dismutation process to generate the end‐product 1,3‐PD and the intermediate metabolite phosphoenolpyruvate (PEP). Generally, the other end‐products derived from PEP are acetate, lactate, succinate and 2,3‐butanediol (2,3‐BD) (Fig.…”

Genetically Engineered Strains: Application and Advances for 1,3-Propanediol Production from Glycerol

“…Hence, the decreased intermediates cause a low titre of the target product. This process triggers irreversible cessation of metabolic activity, which becomes a serious limitation to the 1,3-PD production process (31).…”

“…Apart from the fact that the stability of the enzyme is increased, cell immobilization also makes it possible to maintain the primitive state and high recovery rate of the enzyme, and the coenzyme factor can be added automatically. It has been demonstrated that cell immobilization techniques can significantly increase 1,3-PD production and allow robust continuous production (31). Therefore, fermentation by using immobilized microorganisms is a great way to keep the longevity and stability of the biomass.…”

“…The second is to carry out stress treatment by adding the inhibitors like glycerol and 1,3-PD into the fermentation broth (68). However, the most promising technique is immobilization of cells (31). The cells are fixed into a solid matrix or retained by a membrane.…”