Cell immobilization on 3D-printed matrices: A model study on propionic acid fermentation

Belgrano, Fabricio S.; Diegel, Olaf; Pereira, Nei; Hatti‐Kaul, Rajni

doi:10.1016/j.biortech.2017.10.087

Cited by 41 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Propanoic acid (0.53%) is an important chemicals, and it is also the intermediate of many other fine chemicals (Bodulev et al, 2018). It is mainly used as food and feed additives, followed by home medicine, rare herbs, medicine, and spices (Belgrano et al, 2018, Nazareth et al, 2018). In terms of grain and feed additives, the application of propionic acid is significant and consumption is growing rapidly (Kim et al, 2018a, Kim et al, 2018b).…”

Section: Results and Analysismentioning

confidence: 99%

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Chen

Yue

Yang

et al. 2019

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Torreya grandis is a unique tree species in China. Although full use has been made of the timber, the processing and utilization of the bark has not been effective. In order to explore a new way to utilize the bark of Torreya grandis , a powder of T. grandis bark was prepared and analyzed qualitatively and quantitatively. Differential scanning calorimetry (TG) and pyrolysis gas chromatography-mass spectrometry (PY-GC/MS) revealed many bioactive components in the bark of T. grandis, such as acetic acid, 2-methoxy-4-vinyl phenol, D-mannose, and furfural. These substances have potential broad applications in the chemical industry, biomedicine, and food additives. The chemical constituents of the bark of T. grandis suggest a theoretical basis for the future development and utilization of the bark of T. grandis .

show abstract

Section: Results and Analysismentioning

confidence: 99%

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Chen

Yue

Yang

et al. 2019

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Additionally, the waste of material is minimal. 186 These advantages reduce the time needed for process development showing an easy and well-planned route to 'rapid prototyping'. 187 The fusion between 3D printing and continuous flow biocatalysis took place few years ago when researchers started to ask themselves how additive manufacturing's pros enhance continuous flow chemistry.…”

Section: Additive Manufacturing/3d Printingmentioning

confidence: 99%

“…190 The broad applicability of 3D-printed carriers has already been investigated with different enzymes and under different conditions, indicating the universal applicability of those carrier materials and processes. 186,191,192 Entrapping enzymes in 3D-printed hydrogel beads creates an optimal aqueous environment restricting the movement of macromolecules while substrates and products can pass through the hydrogel network. This hydrogel network can be Reaction Chemistry & Engineering Review specifically designed by a careful selection of monomers and cross-linkers.…”

Section: Additive Manufacturing/3d Printingmentioning

confidence: 99%

“…185,188 Various types of inks have already been evaluated for enzyme immobilization strategies, including the most promising ones based on poly(ethylene glycol) diacrylate, 190 and nylon. 186 Recently, Liese and co-workers investigated polyethylene terephthalate (PET) as an additively manufactured carrier material for the immobilization of phenolic acid decarboxylase (PAD) from Mycobacterium colombiense for the decarboxylation of ferulic acid. PAD was genetically fused with an anchor peptide and non-covalently immobilized on the support PET.…”

Section: Additive Manufacturing/3d Printingmentioning

confidence: 99%

See 1 more Smart Citation

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

2020

View full text Add to dashboard Cite

show abstract

“…Cell immobilization technology may address the issues. Cell entrapment is a typical immobilization approach with advantages that include high cell density, easy recyclability, and facile preparation (dos Santos Belgrano et al, 2018). The use of calcium alginate for cell entrapment has attracted considerable attention because of its good biocompatibility and easy preparation (Ha et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Cofactor Self-Sufficient Recombinant Escherichia coli for Enantioselective Biosynthesis of (R)-1-Phenyl-1,2-Ethanediol

Peng

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

phenyl-1,2-ethanediol is an important synthon for the preparation of β-adrenergic blocking agents. This study identified a (2R,3R)-butanediol dehydrogenase (KgBDH) from Kurthia gibsonii SC0312, which showed high enantioselectivity for production of (R)-1-phenyl-1,2-ethanediol by reduction of 2-hydroxyacetophenone. KgBDH was expressed in a recombinant engineered strain, purified, and characterized. It showed good catalytic activity at pH 6-8 and better stability in alkaline (pH 7.5-8) than an acidic environment (pH 6.0-7.0), providing approximately 73 and 88% of residual activity after 96 h at pH 7.5 and 8.0, respectively. The maximum catalytic activity was obtained at 45 • C; nevertheless, poor thermal stability was observed at >30 • C. Additionally, the examined metal ions did not activate the catalytic activity of KgBDH. A recombinant Escherichia coli strain coexpressing KgBDH and glucose dehydrogenase (GHD) was constructed and immobilized via entrapment with a mixture of activated carbon and calcium alginate via entrapment. The immobilized cells had 1.8-fold higher catalytic activity than that of cells immobilized by calcium alginate alone. The maximum catalytic activity of the immobilized cells was achieved at pH 7.5, and favorable pH stability was observed at pH 6.0-9.0. Moreover, the immobilized cells showed favorable thermal stability at 25-30 • C and better operational stability than free cells, retaining approximately 55% of the initial catalytic activity after four cycles. Finally, 81% yields (195 mM product) and >99% enantiomeric excess (ee) of (R)-1-phenyl-1,2-ethanediol were produced within 12 h through a fed-batch strategy with the immobilized cells (25 mg/ml wet cells) at 35 • C and 180 rpm, with a productivity of approximately 54 g/L per day.

show abstract

Cell immobilization on 3D-printed matrices: A model study on propionic acid fermentation

Cited by 41 publications

References 32 publications

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

Immobilization of Cofactor Self-Sufficient Recombinant Escherichia coli for Enantioselective Biosynthesis of (R)-1-Phenyl-1,2-Ethanediol

Contact Info

Product

Resources

About