Cell Inertia: Predicting Cell Distributions in Lung Vasculature to Optimize Re-endothelialization

Chan, Jason K D; Chadwick, Eric Alexander; Taniguchi, Daisuke; Ahmadipour, Mohammadali; Suzuki, Takaya; Romero, David A.; Amón, Cristina H.; Waddell, Thomas K.; Karoubi, Golnaz; Bazylak, Aimy

doi:10.3389/fbioe.2022.891407

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…Previous studies have utilized decellularized mouse lungs for cell repopulation; however, the culture conditions were either static using decellularized lung slices 10 or not physiologically relevant where mouse lungs were incubated in a rotating syringe 11 . Recent literature has reported that a mouse-scale dynamic culture platform could enhance the repopulation of epithelial cells in decellularized mouse lung scaffolds, which led us to extend the platform to vascular engineering with proper vascular access 12,13 . Herein, we expand on this platform to optimize and evaluate the endothelialization of acellular mouse lung scaffolds.…”

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confidence: 99%

Orthotopic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells

Tomiyama,

Suzuki,

Watanabe

et al. 2024

Sci Rep

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Whole lung engineering and the transplantation of its products is an ambitious goal and ultimately a viable solution for alleviating the donor-shortage crisis for lung transplants. There are several limitations currently impeding progress in the field with a major obstacle being efficient revascularization of decellularized scaffolds, which requires an extremely large number of cells when using larger pre-clinical animal models. Here, we developed a simple but effective experimental pulmonary bioengineering platform by utilizing the lung as a scaffold. Revascularization of pulmonary vasculature using human umbilical cord vein endothelial cells was feasible using a novel in-house developed perfusion-based bioreactor. The endothelial lumens formed in the peripheral alveolar area were confirmed using a transmission electron microscope. The quality of engineered lung vasculature was evaluated using box-counting analysis of histological images. The engineered mouse lungs were successfully transplanted into the orthotopic thoracic cavity. The engineered vasculature in the lung scaffold showed blood perfusion after transplantation without significant hemorrhage. The mouse-based lung bioengineering system can be utilized as an efficient ex-vivo screening platform for lung tissue engineering.

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mentioning

confidence: 99%

Orthotopic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells

Tomiyama,

Suzuki,

Watanabe

et al. 2024

Sci Rep

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Orthotropic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells

Suzuki,

Tomiyama,

Watanabe

et al. 2023

Preprint

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Whole lung engineering and the transplantation of its products is an ambitious goal and ultimately a viable solution for alleviating the donor-shortage crisis for lung transplants. There are several limitations currently impeding progress in the field with a major obstacle being efficient revascularization of decellularized scaffolds, which requires an extremely large number of cells when using larger pre-clinical animal models. Here, we developed a simple but effective experimental pulmonary bioengineering platform by utilizing the lung as a scaffold. Revascularization of pulmonary vasculature using human umbilical cord vein endothelial cells was feasible using a novel in-house developed perfusion-based bioreactor. The endothelial lumens formed in the peripheral alveolar area were confirmed using a transmission electron microscope. The quality of engineered lung vasculature was evaluated using fractal dimension analysis of histological images. The engineered mouse lungs were successfully transplanted into the orthotopic thoracic cavity. The engineered vasculature in the lung scaffold showed blood perfusion after transplantation without significant hemorrhage. The mouse-based lung bioengineering system can be utilized as an efficient ex-vivo screening platform for lung tissue engineering.

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Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Mazloomnejad

Babajani

Kasravi

et al. 2023

Front. Bioeng. Biotechnol.

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Decellularization of tissues and organs has recently become a promising approach in tissue engineering and regenerative medicine to circumvent the challenges of organ donation and complications of transplantations. However, one main obstacle to reaching this goal is acellular vasculature angiogenesis and endothelialization. Achieving an intact and functional vascular structure as a vital pathway for supplying oxygen and nutrients remains the decisive challenge in the decellularization/re-endothelialization procedure. In order to better understand and overcome this issue, complete and appropriate knowledge of endothelialization and its determining variables is required. Decellularization methods and their effectiveness, biological and mechanical characteristics of acellular scaffolds, artificial and biological bioreactors, and their possible applications, extracellular matrix surface modification, and different types of utilized cells are factors affecting endothelialization consequences. This review focuses on the characteristics of endothelialization and how to optimize them, as well as discussing recent developments in the process of re-endothelialization.

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Cell Inertia: Predicting Cell Distributions in Lung Vasculature to Optimize Re-endothelialization

Cited by 3 publications

References 61 publications

Orthotopic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells

Orthotopic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells

Orthotropic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

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