Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Guerrero, Julien; Catros, Sylvain; Derkaoui, Sidi-Mohammed; Lalande, Charlotte; Siadous, Robin; Bareille, Reine; Thebaud, Noëlie; Bordenave, Laurence; Chassande, Olivier; Visage, Catherine Le; Letourneur, Didier; Amédée, Joëlle

doi:10.1016/j.actbio.2013.05.025

Cited by 70 publications

(76 citation statements)

References 77 publications

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“…[ 119,120 ] Various biomaterials have been developed for transplantation of stem cells and their specifi c differentiation into bones, cartilages, and muscles. [ 119,[121][122][123] One of the vital aims for bone regeneration is to direct the proliferation of stem cells and to promote their differentiation in a controlled manner with the use of osteogenic inducers and growth factors. [ 124,125 ] To repair and regenerate bone tissue, the use of graphene-based materials and stem cells is being studied and the effective promotion of the osteogenic differentiation of MSCs is one of the core issues in this fi eld.…”

Section: Supporting In Vitro Osteogenic Differentiation Of Mesenchymamentioning

confidence: 99%

Graphene‐Based Materials in Regenerative Medicine

Ding

Liu

Fan

2015

Adv Healthcare Materials

147

100

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Graphene possesses many unique properties such as two-dimensional planar structure, super conductivity, chemical and mechanical stability, large surface area, and good biocompatibility. In the past few years, graphene-based materials have risen as a shining star on the path of researchers seeking new materials for future regenerative medicine. Herein, the recent research advances made in graphene-based materials mostly utilizing the mechanical and electrical properties of graphene are described. The most exciting findings addressing the impact of graphene-based materials on regenerative medicine are highlighted, with particular emphasis on their applications including nerve, bone, cartilage, skeletal muscle, cardiac, skin, adipose tissue regeneration, and their effects on the induced pluripotent stem cells. Future perspectives and emerging challenges are also addressed in this Review article.

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Section: Supporting In Vitro Osteogenic Differentiation Of Mesenchymamentioning

confidence: 99%

Graphene‐Based Materials in Regenerative Medicine

Ding

Liu

Fan

2015

Adv Healthcare Materials

147

100

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“…In addition, these matrices have recently been shown to favor the co-culture of human MSCs and human EPCs, promoting cell communication and improved bone formation [50].…”

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confidence: 99%

The Use of Total Human Bone Marrow Fraction in a Direct Three-Dimensional Expansion Approach for Bone Tissue Engineering Applications: Focus on Angiogenesis and Osteogenesis

Guerrero

Oliveira

Catros

et al. 2015

Tissue Engineering Part A

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Current approaches in bone tissue engineering have shown limited success, mostly owing to insufficient vascularization of the construct. A common approach consists of co-culture of endothelial cells and osteoblastic cells. This strategy uses cells from different sources and differentiation states, thus increasing the complexity upstream of a clinical application. The source of reparative cells is paramount for the success of bone tissue engineering applications. In this context, stem cells obtained from human bone marrow hold much promise. Here, we analyzed the potential of human whole bone marrow cells directly expanded in a three-dimensional (3D) polymer matrix and focused on the further characterization of this heterogeneous population and on their ability to promote angiogenesis and osteogenesis, both in vitro and in vivo, in a subcutaneous model. Cellular aggregates were formed within 24 h and over the 12-day culture period expressed endothelial and bone-specific markers and a specific junctional protein. Ectopic implantation of the tissue-engineered constructs revealed osteoid tissue and vessel formation both at the periphery and within the implant. This work sheds light on the potential clinical use of human whole bone marrow for bone regeneration strategies, focusing on a simplified approach to develop a direct 3D culture without two-dimensional isolation or expansion.

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“…Bone healing can be improved by adjusting the speed of vascularization and during the last 5 years, attempts have been made to increase vascularization in the tissue-engineering field [27-29, 33, 38-39]. Hence, basic strategies using scaffolds focused firstly on the use of cells (HUVEC, EPC) [30][31][32], and secondly on the use of active molecules (VEGF, Ang1, ...) operating on endothelial cells [34][35][36] to promote proliferation, migration, sprouting and establishment of functional blood vessel. In our strategy, we chose to use not only VEGF active molecules (VEGF-NPs), but also endothelial and mesenchymal cells.…”

Section: Double Effect Of Vegf-nps Active 3d Pcl and Hob On Endothelimentioning

confidence: 99%

“…Consequently, during the last few years, tridimensional scaffolds associated with endothelial cells were developed for the cell-based strategy with the goal to promote biomaterials vascularization [33][34][35][36]. Various studies have reported the development of VEGF loaded nanoparticles for bone regeneration, wound healing angiogenesis, or inhibition of the graft shrinkage [40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

“…The prosthesis domain aims for replacement after ablation due to severe injuries, the bone substitute domain, targets the substitution of important lesions (until 60 cm [27][28][29]33]. In order to increase vascularization within the implanted site, several active angiogenic materials were developed with majority based on cell activities [34][35][36] or added factors activity [30][31][32]. One of the main growth factor involved in angiogenesis is VEGF165 (Vascular Endothelial Growth Factor) which acts as an initiator and a modulator of the signaling cascade resulting in proliferation and migration of endothelial cells toward formation of new blood vessels [37,38].…”

Section: Introductionmentioning

confidence: 99%

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