Cell isolation and growth in electric-field defined micro-wells

Arnold, W. Mike; Franich, Nick R.

doi:10.1016/j.cap.2005.11.021

Cited by 15 publications

(9 citation statements)

References 13 publications

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“…In one of the first reports, the manipulation of yeast cells by means of DEP forces was described by Crane and Pohl (Crane and Pohl 1968). Since then, different DEP systems have been introduced with the capability of patterning and manipulation of yeast cells (Arnold and Franich 2006;Kadaksham et al 2005;Morales et al 2008), the separation of the live from dead yeast cells (Arnold 2001;Fatoyinbo et al 2005;Li et al 2007;Markx et al 1994) and yeast cells from polystyrene microparticles (Bhatt et al 2005;Hunt et al 2004;Lee et al 2007). Fu et al (2004) applied the travelling wave DEP force rather than the classical DEP force to manipulate the yeast cells.…”

Section: Introductionmentioning

confidence: 97%

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khoshmanesh

Zhang

Tovar-Lopez

et al. 2009

Microfluid Nanofluid

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This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of *80% at low medium conductivities and *85% at high medium conductivities.

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Section: Introductionmentioning

confidence: 97%

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khoshmanesh

Zhang

Tovar-Lopez

et al. 2009

Microfluid Nanofluid

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“…Research has been reported on genetic profiling analysis after DEP that suggests that DEP does not stress mammalian cells to the point that their viability is compromised . Studies on yeast cell viability during the exposure to DEP have been reported . Therefore, the current knowledge is that the DEP forces operate close to the limit of cell viability and it is a matter of debate whether the conditions used in DEP experiments might induce alteration in cell membrane potentials and structures, as well as in their internal metabolic machinery.…”

Section: Introductionmentioning

confidence: 99%

Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics

et al. 2013

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The spatial and temporal control of biological species is essential in complex microfluidic biosystems. In addition, if the biological species is a cell, microfluidic handling must ensure that the cell's metabolic viability is maintained. The use of DEP for cell manipulation in microfluidics has many advantages because it is remote and fast, and the voltages required for cell trapping scale well with miniaturization. In this paper, the conditions for bacterial cell (Escherichia coli) trapping using a quadrupole electrode configuration in a PDMS microfluidic channel were developed both for stagnant and for in-flow fluidic situations. The effect of the electrical conductivity of the fluid, the applied electric field and frequency, and the fluid-flow velocity were studied. A dynamic exchange between captured and free-flowing cells during DEP trapping was demonstrated. The metabolic activity of trapped cells was confirmed by using E. coli cells genetically engineered to express green fluorescent protein under the control of an inducible promoter. Noninduced cells trapped by negative DEP and positive DEP were able to express green fluorescent protein minutes after the inducer was inserted in the microchannel system immediately after DEP trapping. Longer times of trapping prior to exposure to the inducer indicated first a degradation of the cell metabolic activity and finally cell death.

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“…A further possibility for the formation of structured tissue is to isolate cells in electric field traps and allow them to grow in defined positions within an array [54,55]. It is interesting to consider how far the above techniques, or combinations of them, may be effective at assembling nanoparticles into chains of specifiable orientation, or into more complex structures.…”

Section: Introductionmentioning

confidence: 99%

Particle patterning using fluidics and electric fields

Arnold

2008

IEEE Trans. Dielect. Electr. Insul.

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Electric-field manipulation of micro-particles in suspension can create patterns via a number of particle forces and fluid flows. These effects are assessed for their suitability for down-scaling to form nano-patterns such as may be incorporated into structured nano-composites. Consideration is given not only to the assembly of field-aligned chains or wires, but also to methods that can give cross-field assembly and even 2-D patterns or crystals. Dielectrophoresis is often the dominant driving force behind particle assembly, but other dipole-dipole interactions and also electrically-driven fluid flows are increasingly recognized as significant or dominant. Orientation of nonspherical particles in frequency-selectable directions is also possible. Estimates for the threshold field strengths required for using these effects to handle nano-particles are considered. Finally the use of media with modified permittivity to increase the fieldinduced forces or to optimize the selectivity of particle incorporation is discussed. Index Terms -Composite; Artificial tissue; Dielectrophoresis; Electroosmosis W. Michael Arnold (A'95-SM'00) was raised in Yorkshire, U.K. He received the B.A. degree from Cambridge University in 1974, and the M.Sc. and Ph.D. (EE) degrees from the University of Wales.After developing the technique of electro-rotation and also teaching in Biotechnology at the University of Würzburg, Germany, he received the Dr. rer. nat. habil. degree from that University in 1993. In 1995 he chaired the IAS session on "Biological Applications of Electrostatics", and in 2001 he cochaired a DEIS workshop on "Fast Field Effects in Biosystems". Present research interests are nanoassembly, microfluidic devices and bio-impedance spectroscopy.

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Cell isolation and growth in electric-field defined micro-wells

Cited by 15 publications

References 13 publications

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics

Particle patterning using fluidics and electric fields

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