CELL KINETICS IN MOUSE THYMUS STUDIED BY SIMULTANEOUS USE OF <sup>3</sup>H‐THYMIDINE AND COLCHICINE

Borum, Kirstine

doi:10.1111/j.1365-2184.1973.tb01645.x

Cited by 8 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37) and in experimental tumors (38). In the normal lymphoid germinal center and in the normal bone marrow, cell migration and growth retardation are accompanied by a clearly demonstrable morphologic transition from large to small cell size.…”

Section: Discussionmentioning

confidence: 99%

Dual parameter flow cytometry studies in human lymphomas.

Shackney¹,

Skramstad²,

Cunningham³

et al. 1980

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Dual parameter flow cytometry studies using Coulter volume and cell DNA content were carried out in monodisperse cell suspensions of 64 samples of human lymphoma, chronic lymphocytic leukemia, hairy cell leukemia, and benign lymphoid proliferations. Differences in mean Coulter volume among the lymphomas were due 1)oth to the intrinsic differences in mean G1 cell Coulter volume and to the presence of increased fractions of larger S and G2 cells, especially among the large B cell lymphomas.However, the relative contribution of larger non-G, cells to the overall population Coulter volume distribution was a relatively minor one; the presence of cells in S did not increase mean Coulter volume by more than 10%, even in samples with high S fractions. There was a good correlation between mean GC cell Coulter volume and the log of the fraction of cells in S among the B cell lymphomas (r = 0.55). Evidence is presented that within individual samples, large cells proliferate more rapidly than small cells. This was seen in every case, both in the normal samples and in the lymphomas, and in the T cell lymphomas as well as in the B cell lymphomas. Aneuploidy was detected by flow cytometry in 11 cases; in 7 cases the aneuploid cell component could be analyzed separately from the diploid cell component on the basis of cell Coulter volume differences. Predictable relationships between cell size and proliferative behavior might be expected in the lymphomas for at least two reasons.First, cells increase both in size and in DNA content as they progress through the cell cycle. The dependence of cell size on cell position in the proliferative cycle can be demonstrated in experimental cell systems, by performing paired measurements of electronic cell volume and DNA content on individual cells by means of flow cytometry. This is illustrated in Fig. 1. Thus, the frequencies of large cells in a given lymphoma might be determined by the fractions of large S and G2 cells.Second, rapidly proliferating lymphoid cells may be intrinsically larger than slowly proliferating cells. It has been shown that large lymphocytes proliferate more rapidly than small lymphocytes in the normal lymphoid germinal center (8), and in the normal bone marrow (9-11). A relationship between proliferative rate and cell size (which is independent of relative position in the cell cycle) might be preserved in the malignant state as well.Of course, cell size differences among the lymphomas could also be due to factors that are totally unrelated to their respective proliferative rates or to the presence of different fractions of larger S and G2 cells. and DNA content were obtained in monodisperse cell suspensions of pretherapy biopsy specimens of human lymphoma by means of flow cytometry. Conventional histologic studies and immunologic surface marker studies were done in parallel on the same clinical samples. METHODSClinical samples. The collection and processing of pretherapy biopsy specimens and the techniques for determining immunologic cell surface mar...

show abstract

Section: Discussionmentioning

confidence: 99%

Dual parameter flow cytometry studies in human lymphomas.

Shackney¹,

Skramstad²,

Cunningham³

et al. 1980

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Until today, no report has come up which evidences that the majority of lymphocytes produced in the thymus die in situ. In contrast, many autoradiographic studies show that thymocytes move from the cortex to the medulla, from where they may emigrate via the PVS and the blood (BORUM 1973;JOEL et al 1974;AMANO and HAMATANI 1981). Experiments with radioactively labeled thymocytes, however, have demonstrated that 16% of all thymocytes emigrate in mice (JOEL et al 1977).…”

Section: Cellular Proliferation and Cell Deathmentioning

confidence: 99%

“…The proliferative capacity of the thymus of experimental animals has been abundantly investigated during the last two decades (LEBLOND 1959;SAINTE-MARIE and LEBLOND 1964;MURRAY et al 1965a, b;BORUM 1973;SHORTMAN and JACKSON 1974;BELLAMY and HINSULL 1977;HINSUr:L and BELLAMY 1981;AMANO and HAMATANI 1981;ROTHENBERG 1982). It is generally accepted that " x 1000, oil immersion, 500 cells counted thymic homeostasis operates via the control of cell proliferation.…”

Section: Cellular Proliferation and Cell Deathmentioning

confidence: 99%

Changes in the Human Thymus During Aging

Steinmann¹

1986

Current Topics in Pathology

293

184

View full text Add to dashboard Cite

“…As 32 P-orthophosphate is continuously incorporated (figure 1) but only into the DNA of the dividing fraction of cells (Ord and Stocken 1956), the labelling of DNA in the whole population requires about 2 or 3 days . The incorporation of labelled precursors into the DNA of thymus cells by other routes than cell division is improbable (Borum 1973) . The dose-response curves determined at 72 hours after irradiation (figure 2 and table) support the assumption that in the low dose-range the dividing cells from thymus are capable of recovery from radiation injury .…”

Section: Discussionmentioning

confidence: 99%

Recovery Response of Dividing Cells in the Thymus of Whole-body Gamma-irradiated Mice

Suciu

Uray

Maniu

1976

International Journal of Radiation Biology and Related Studies

View full text Add to dashboard Cite

Mice were irradiated with different doses of gamma-rays 30 min after the administration of 32P-orthophosphate. The dose-response curves determined at 72 hours after exposure showed an inflection point in the total activity present in the DNA in thymus and spleen. In the low dose-range, the dose-response curves have D0 = 55 rad (n = 2-5) for thymus and DO = 95 rad (n = 2-5) for the spleen. Thirty minutes after the administration of 32P-orthophosphate, the dividing cells from thymus were partially synchronized by the administration of 80 mg per kg body-weight hydroxyurea. At different time-intervals, the mice were irradiated with 80 rad, and the total activity of DNA was determined at 72 hours after synchronization. A significant maximum of recovery was found at 5 hours (S phase) after the administration of hydroxyurea. In similar conditions, the dose-response curves corresponding to the G1, S and M phase of the division cycle were also determined. The synchronization of dividing cells induced by hydroxyurea failed in the spleen.

show abstract

CELL KINETICS IN MOUSE THYMUS STUDIED BY SIMULTANEOUS USE OF ³H‐THYMIDINE AND COLCHICINE

Cited by 8 publications

References 9 publications

Dual parameter flow cytometry studies in human lymphomas.

Dual parameter flow cytometry studies in human lymphomas.

Changes in the Human Thymus During Aging

Recovery Response of Dividing Cells in the Thymus of Whole-body Gamma-irradiated Mice

Contact Info

Product

Resources

About

CELL KINETICS IN MOUSE THYMUS STUDIED BY SIMULTANEOUS USE OF 3H‐THYMIDINE AND COLCHICINE

Cited by 8 publications

References 9 publications

Dual parameter flow cytometry studies in human lymphomas.

Dual parameter flow cytometry studies in human lymphomas.

Changes in the Human Thymus During Aging

Recovery Response of Dividing Cells in the Thymus of Whole-body Gamma-irradiated Mice

Contact Info

Product

Resources

About

CELL KINETICS IN MOUSE THYMUS STUDIED BY SIMULTANEOUS USE OF ³H‐THYMIDINE AND COLCHICINE