Cell kinetics of hemopoietic colony-forming units in spleen (CFU-S) in young and old mice

Hirabayashi, Yoko; Matsumura, Takuya; Matsuda, Motoi; Kuramoto, Kazunao; Motoyoshi, Kazuo; Yoshida, Kazuko; Sasaki, Hideki; Inoue, Tohru

doi:10.1016/s0047-6374(97)00175-9

Cited by 16 publications

(20 citation statements)

References 18 publications

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“…All the types of HPC of the nonirradiated control groups showed curves compatible with previous observations, 10,11 i.e., the percent incorporation of BrdUrd increased logarithmically with BrdUrd treatment duration and reached a plateau on day 3 or 5. Compared with the nonirradiated control groups, the irradiated groups showed a significantly higher percent BrdUrd incorporation at 1 h but flatter slopes of the extrapolation curves in the log phase of increasing ratio.…”

Section: Hematological Parameter Recovery 4 Weeks After Whole-body Irsupporting

confidence: 89%

“…10 Moreover, it was reported that beta-catenin, cyclin D1, and PiK3r1 appeared to be downregulated in bone marrow cells of aged mice, 2 suggesting that hematopoiesis in the old group was suppressed in the steady state, specifically that in immature HPCs. In addition, although there was no significant ....................................................................................................................... difference in the cell cycle of CFU-GM or CFU-S9 between the nonirradiated young and old groups, the irradiated young and old groups showed marked differences in the cell-cycle parameters of HPCs.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11][12] Accordingly, the percent mortality of BrdUrd-incorporating HPCs purged with UVA light corresponds to the percent fraction of cells that had incorporated BrdUrd. Figure 1 shows the experimental design.…”

Section: Brdurd-ultraviolet-light Cytocide Assaymentioning

confidence: 99%

“…8,9 In contrast, however, nonirradiated old mice show deceleration of the cell cycle of primitive HSCs/HPCs. 10 The cell cycle assay of hematopoietic colony-forming progenitor cells was carried out in this study by continuous bromodeoxyuridine (BrdUrd) incorporation in vivo and ultraviolet A (UVA) light irradiation to purge cells that incorporated BrdUrd (BUUV assay). The assay specifically provides various cell-cycle parameters, such as the percent incorporation of BrdUrd at time zero, generation doubling time, and the cycling-cell fraction accumulated during BrdUrd incorporation, which is in contrast to the dormant cell fraction, in certain HPCs.…”

Section: Introductionmentioning

confidence: 99%

“…The assay specifically provides various cell-cycle parameters, such as the percent incorporation of BrdUrd at time zero, generation doubling time, and the cycling-cell fraction accumulated during BrdUrd incorporation, which is in contrast to the dormant cell fraction, in certain HPCs. [10][11][12] By this BUUV assay, we evaluated cell kinetic changes with aging 10 and the effects of benzene inhalation. 12,13 In addition, the effects of various genes related to HSC maintenance were also evaluated using mice with modified genes, e.g., Trp53, 11,14 aryl hydrocarbon receptor, 15,16 thioredoxin, 17,18 and connexin 32 [19][20][21] genes.…”

Section: Introductionmentioning

confidence: 99%

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Cell cycle of primitive hematopoietic progenitors decelerated in senescent mice is reactively accelerated after 2-Gy whole-body irradiation

Hirabayashi

Tsuboi

Kuramoto

et al. 2016

Exp Biol Med (Maywood)

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Aging is considered to be a functional retardation of continuous xenobiotic responses over a lifetime after the developmental period; thus, the effects of ionizing radiation over a lifetime may be somewhat accounted for by a modifier of aging effects. This study was conducted to evaluate the possible/synergic effects of radiation during aging by determining cell-cycle parameters of hematopoietic stem cells/hematopoietic progenitor cells (HSCs/HPCs), such as the percent of cells in cycling, the generation doubling time, and the cumulative cycling-cell fraction, by bromodeoxyuridine-ultraviolet assay, which enables the determination of their cycling capacity in vivo. Colony-forming progenitor cells, such as colony-forming unit (CFU)-granulocyte/macrophage (GM), CFU in the spleen on day 9 (CFU-S9), and CFU-S on day 13 (CFU-S13) for mature, less mature, and immature HPCs, respectively, were evaluated in young and old mice (6 weeks and 21 months of age, respectively) with or without 2-Gy whole-body irradiation and a 4-week recovery period. Then, cell-cycle parameters were evaluated and compared. As a result, the generation doubling time of all types of HPC was prolonged by the irradiation in both young and old mouse groups, except that of CFU-S13 in old mice, which showed acceleration of the cell cycle following the irradiation. In addition, only CFU-S13 in irradiated old mice showed a significant increase in the cumulative cycling-cell-fraction ratio. Significant changes due to the effects of aging and irradiation on HPCs were observed only in the immature HPCs, i.e., the cell cycle of immature HPCs was suppressed by aging without irradiation and was, in contrast, accelerated as the cells recovered from radiation-induced damage. This suggests that the mechanisms of peripheral blood recovery after 2-Gy whole-body irradiation are markedly different between young and old mice, although 21-month-old mice showed almost the same level of recovery as the young mice.

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Section: Hematological Parameter Recovery 4 Weeks After Whole-body Irsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Brdurd-ultraviolet-light Cytocide Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cell cycle of primitive hematopoietic progenitors decelerated in senescent mice is reactively accelerated after 2-Gy whole-body irradiation

Hirabayashi

Tsuboi

Kuramoto

et al. 2016

Exp Biol Med (Maywood)

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Toxicogenomics Applied to Hematotoxicology

Hirabayashi

Inoue

2005

Handbook of Toxicogenomics

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Membrane Channel Connexin 32 Maintains Lin−/c-kit+ Hematopoietic Progenitor Cell Compartment: Analysis of the Cell Cycle

Hirabayashi

Yoon

Tsuboi³

et al. 2007

J Membrane Biol

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Membrane channel connexin (Cx) forms gap junctions that are implicated in the homeostatic regulation of multicellular systems; thus, hematopoietic cells were assumed not to express Cxs. However, hematopoietic progenitors organize a multicellular system during the primitive stage; thus, the aim of the present study was to determine whether Cx32, a member of the Cx family, may function during the primitive steady-state hematopoiesis in the bone marrow (BM). First, the numbers of mononuclear cells in the peripheral blood and various hematopoietic progenitor compartments in the BM decreased in Cx32-knockout (KO) mice. Second, on the contrary, the number of primitive hematopoietic progenitor cells, specifically the Lin(-)/c-kit(+)/Scal(+ )fraction, the KSL progenitor cell compartment, also increased in Cx32-KO mice. Third, expression of Cx32 was detected in Lin(-)/c-kit(+) hematopoietic progenitor cells of wild-type mice (0.27% in the BM), whereas it was not detected in unfractionated wild-type BM cells. Furthermore, cell-cycle analysis of the fractionated KSL compartment from Cx32-KO BM showed a higher ratio in the G(2)/M fraction. Taken together, all these results imply that Cx32 is expressed solely in the primitive stem cell compartment, which maintains the stemness of the cells, i.e., being quiescent and noncycling; and once Cx32 is knocked out, these progenitor cells are expected to enter the cell cycle, followed by proliferation and differentiation for maintaining the number of peripheral blood cells.

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Cell kinetics of hemopoietic colony-forming units in spleen (CFU-S) in young and old mice

Cited by 16 publications

References 18 publications

Cell cycle of primitive hematopoietic progenitors decelerated in senescent mice is reactively accelerated after 2-Gy whole-body irradiation

Cell cycle of primitive hematopoietic progenitors decelerated in senescent mice is reactively accelerated after 2-Gy whole-body irradiation

Toxicogenomics Applied to Hematotoxicology

Membrane Channel Connexin 32 Maintains Lin−/c-kit+ Hematopoietic Progenitor Cell Compartment: Analysis of the Cell Cycle

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