Cell-laden Polymeric Microspheres for Biomedical Applications

Leong, Wenyan; Wang, Dong‐An

doi:10.1016/j.tibtech.2015.09.003

Cited by 96 publications

(71 citation statements)

References 88 publications

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“…142 Moreover, compared with monolayer cultures, the expansion and differentiation of encapsulated stem cells can be accomplished efficiently in a one-step process in microfluidic microgels. 135 Furthermore, the monodispersity of microfluidic droplets is particularly important in microscale tissue engineering, in which the spheroid size influences stem cell behavior and differentiation potential. 143,144 …”

Section: Cell-laden Microfluidic Microgels For Tissue Regenerationmentioning

confidence: 99%

Cell-laden microfluidic microgels for tissue regeneration

et al. 2016

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Regenerating the diseased tissue is one of the foremost concerns for the millions of patients who suffer from tissue damage each year. Local delivery of cell-laden hydrogels offers an attractive approach for tissue repair. However, due to the typical macroscopic size of these cell constructs, the encapsulated cells often suffer from poor nutrient exchange. These issues can be mitigated by incorporating cells into microscopic hydrogels, or microgels, whose large surface-to-volume ratio promotes efficient mass transport and enhanced cell-matrix interactions. Using microfluidic technology, monodisperse cell-laden microgels with tunable sizes can be generated in a high-throughput manner, making them useful building blocks that can be assembled into tissue constructs with spatially controlled physicochemical properties. In this review, we examine microfluidics-generated cell-laden microgels for tissue regeneration applications. We provide a brief overview of the common biomaterials, gelation mechanisms, and microfluidic device designs that are used to generate these microgels, and summarize the most recent works on how they are applied to tissue regeneration. Finally, we discuss future applications of microfluidic cell-laden microgels as well as existing challenges that should be resolved to stimulate their clinical application.

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Section: Cell-laden Microfluidic Microgels For Tissue Regenerationmentioning

confidence: 99%

Cell-laden microfluidic microgels for tissue regeneration

et al. 2016

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“…Several studies have shown that adhesive cues control the environment of transplanted cells, impact their survival, and enhance their ability to form new tissue structures [270][271][272][273]. Photocrosslinkable chitosan hydrogels were endowed with an Ang-1-derived peptide (Gln-His-Arg-Glu-Asp-Gly-Ser, QHREDGS) to enhance the survival and retention of neonatal rat CMs in vitro and in vivo [274].…”

Section: Synthetic Biodegradable Polyestersmentioning

confidence: 99%

“…A variety of exogenous growth factors have been identified to play a considerable role in stem cell differentiation and proliferation in vitro. Cytokines, chemokines, morphogens, and growth factors that regulate survival, proliferation, and/or differentiation may be incorporated in cell vehicles [273]. These bioactive cues may be encapsulated, conjugated, or physically absorbed with cell vehicles and can be spatially and temporally released depending upon the dose required or target tissue [34,281].…”

Section: Influencing Stem Cell Differentiationmentioning

confidence: 99%

Insight on stem cell preconditioning and instructive biomaterials to enhance cell adhesion, retention, and engraftment for tissue repair

Shafiq

Jung

Kim³

2016

Biomaterials

108

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“…Novel carriers hold great promise in achieving enhanced bioavailability, reduced cytotoxicity, targeted delivery, controlled release, and improved efficacy that can ultimately result in desired therapeutic responses in the body. Among these carriers, micro- (Kurmi et al, 2010;Leong and Wang, 2015;Skorb and Möhwald, 2014) and nano- (Gref et al, 2012;Peer et al, 2007;Schroeder et al, 2010;Wang et al, 2012) structures have attracted significant research interest because of their tunability in physical (size, structures, porosity, and mechanical strength) and chemical (compositions, reactivity, biocompatibility, and biodegradability) properties, and flexibility in integrating different functions, such as for active/passive targeting, stimuli-responsive release, and diagnostics/imaging. To enable their full potential in practical pharmaceutical applications, the development of advanced and robust platform technologies for the manufacture of microand nanostructured materials with high degree of uniformity (size, size distribution, and shape), batch-to-batch reproducibility and scale-up possibilities, becomes highly essential.…”

Section: Introductionmentioning

confidence: 99%

Multiphase microfluidic synthesis of micro- and nanostructures for pharmaceutical applications

Ran

Sun

Baby

et al. 2017

Chemical Engineering Science

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Multiphase microfluidics has attracted significant interest in making micro-and nanostructures for various applications because of its capabilities in precisely controlling and manipulating a small volume of liquids. In this review, we introduce the recent advances in making micro-and nanostructures for pharmaceutical applications, including microparticles and microcapsules for controlled release, nanoparticles for drug delivery and microgels for 3D cell culture. With the development of more advanced microfluidic systems, the research focus in this field has shifted from making simple micro-and nanostructures to multifunctional systems to achieve more desirable functions. However, these multifunctions may lose their advantages that have been demonstrated in vitro once they are applied in vivo or later in human. The key challenge is a lack of fundamental understanding of the interactions between the micro-and nanomaterials and the biology systems. Consequently, the translation of these advanced materials lags far behind their extensive laboratory research.To better understand the micro-or nano-bio interactions, the development of new in vivomimicking models is imperative. Microfluidics has demonstrated its great potential in creating physiologically relevant models including 3D cell culture, tumor-on-a-chip and organs-on-a-chip. Therefore, efforts towards developing 3D cell culture and biomimetic chips including tumor-on-a-chip and organs-on-chips for faster and reliable evaluation of these micro-and nanosystems are also highlighted.

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Cell-laden Polymeric Microspheres for Biomedical Applications

Cited by 96 publications

References 88 publications

Cell-laden microfluidic microgels for tissue regeneration

Cell-laden microfluidic microgels for tissue regeneration

Insight on stem cell preconditioning and instructive biomaterials to enhance cell adhesion, retention, and engraftment for tissue repair

Multiphase microfluidic synthesis of micro- and nanostructures for pharmaceutical applications

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