Cell line-dependent cytotoxicity of poly(isobutylcyanoacrylate) nanoparticles coated with chitosan and thiolated chitosan: Insights from cultured human epithelial HeLa, Caco2/TC7 and HT-29/MTX cells

Pradines, Bénédicte; Moal, Vanessa Liévin-Le; Vauthier, Christine; Ponchel, Gilles; Loiseau, Philippe M.; Bouchemal, Kawthar

doi:10.1016/j.ijpharm.2015.06.001

Cited by 15 publications

(13 citation statements)

References 16 publications

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“…In earlier work we have reported on the pronounced permeability enhancing effect of chitosan solutions (Hagesaether, 2011), but this effect seems to diminish when liposomes are coated with chitosan, in line with earlier studies on chitosan nanoparticles (Hafner et al, 2015). This effect might also have been reflected in the biocompatibility, as chitosan in a free soluble form is reported to be much more cytotoxic than when it is incorporated in a nanosystem, arguing for an acceptable cytotoxicity profile of chitosan nanoparticles (Hafner et al, 2015;Pradines et al, 2015).…”

Section: Biocompatibilitysupporting

confidence: 82%

“…The toxicity of poly(isobutyl cyanoacrylate) nanoparticles has recently been found to be highly cell line-dependent: a lower toxicity was reported using human fully-differentiated enterocyte-like Caco-2/TC7, and fullydifferentiated mucus-secreting HT-29/MTX cells forming monolayer in culture, compared to undifferentiated human cervix epithelial HeLa cells. This was attributed to a resistance against internalization by the robust monolayers by tight assembly of polarized cells, mimicking the intestinal epithelial barrier (Pradines et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

Adamczak

Hagesæther

Smistad

et al. 2016

International Journal of Pharmaceutics

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Drug delivery to the oral cavity poses a significant challenge due to the short residence time of the formulations at the site of action. From this point of view, nanoparticulate drug delivery systems with ability to adhere to the oral mucosa are advantageous as they could increase the effectiveness of the therapy. Positively, negatively and neutrally charged liposomes were coated with four different types of polymers: alginate, low-ester pectin, chitosan and hydrophobically modified ethyl hydroxyethyl cellulose. The mucoadhesion was studied using a novel in vitro method allowing the liposomes to interact with a mucus-producing confluent HT29-MTX cell-line without applying any external force. MTT viability and paracellular permeability tests were conducted on the same cell-line. The alginate-coated liposomes achieved a high specific (genuine) mucin interaction, with a low potential of cell-irritation. The positively charged uncoated liposomes achieved the highest initial mucoadhesion, but also displayed a higher probability of cell-irritation. The chitosan-coated liposomes displayed the highest potential for long lasting mucoadhesion, but with the drawback of a higher general adhesion (tack) and a higher potential for irritating the cells.

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Section: Biocompatibilitysupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

Adamczak

Hagesæther

Smistad

et al. 2016

International Journal of Pharmaceutics

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“…We produced an array of nanoparticles covering a range of properties and selected cell lines frequently used in the literature. Cytotoxicity has been shown to be very cell-line dependent [ 10 , 17 ]. This might be due to differences between cell lines in their ability to interact and endocytose the nanoparticles, and in the fact that cancer cell lines in particular often have deregulated pathways of cell signaling, cell death (apoptosis), and autophagy [ 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although there are numerous studies describing the cytotoxicity of PACA NPs [ 10 , 11 , 12 ], the results generally lack consistency and evaluate only a subset of the PACA NP family. Currently, the literature is lacking a comprehensive and systematic study comparing cytotoxicity of various PACA NPs and the dependency on cell lines.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles

Sulheim

Iversen

Nakstad

et al. 2017

IJMS

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Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batches. Here we report the toxicity of poly(alkyl cyanoacrylate) nanoparticles in 12 different cell lines after synthesizing and analyzing 19 different nanoparticle batches and report that large variations were obtained when using different cell lines or various toxicity assays. Surprisingly, we found that nanoparticles with intermediate degradation rates were less toxic than particles that were degraded faster or more slowly in a cell-free system. The toxicity did not vary significantly with either the three different combinations of polyethylene glycol surfactants or with particle size (range 100–200 nm). No acute pro- or anti-inflammatory activity on cells in whole blood was observed.

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“…Another study focused on the capacity of drug unloaded poly (isobutylcyanoacrylate) nanoparticles coated with chitosan (Cs-NPs) to be active after topical application [ 76 ]. No cytotoxicity was observed with this formulation on fully differentiated Caco/TC7 and HT29/MTX cells at 25 µg/mL [ 77 ]. In vitro and in vivo evaluation demonstrated an intrinsic antileishmanial activity of Cs-NPs.…”

Section: Chitosan-based Drug Loaded Formulations For the Chemothermentioning

confidence: 99%

Chitosan Contribution to Therapeutic and Vaccinal Approaches for the Control of Leishmaniasis

2020

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The control of leishmaniases, a complex parasitic disease caused by the protozoan parasite Leishmania, requires continuous innovation at the therapeutic and vaccination levels. Chitosan is a biocompatible polymer administrable via different routes and possessing numerous qualities to be used in the antileishmanial strategies. This review presents recent progress in chitosan research for antileishmanial applications. First data on the mechanism of action of chitosan revealed an optimal in vitro intrinsic activity at acidic pH, high-molecular-weight chitosan being the most efficient form, with an uptake by pinocytosis and an accumulation in the parasitophorous vacuole of Leishmania-infected macrophages. In addition, the immunomodulatory effect of chitosan is an added value both for the treatment of leishmaniasis and the development of innovative vaccines. The advances in chitosan chemistry allows pharmacomodulation on amine groups opening various opportunities for new polymers of different size, and physico-chemical properties adapted to the chosen routes of administration. Different formulations have been studied in experimental leishmaniasis models to cure visceral and cutaneous leishmaniasis, and chitosan can act as a booster through drug combinations with classical drugs, such as amphotericin B. The various architectural possibilities given by chitosan chemistry and pharmaceutical technology pave the way for promising further developments.

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Cell line-dependent cytotoxicity of poly(isobutylcyanoacrylate) nanoparticles coated with chitosan and thiolated chitosan: Insights from cultured human epithelial HeLa, Caco2/TC7 and HT-29/MTX cells

Cited by 15 publications

References 16 publications

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles

Chitosan Contribution to Therapeutic and Vaccinal Approaches for the Control of Leishmaniasis

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