Cell lineage in vascularized bone transplantation

Willems, Wouter F.; Larsen, Mikko; Friedrich, Patricia F.; Bishop, Allen T.

doi:10.1002/micr.22147

Cited by 2 publications

(2 citation statements)

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“…Previously, we applied fluorescent labeling, laser capture microdissection, and quantitative rtPCR to study cell lineage in bone autografts and allotransplants and described detailed spatial changes in cell lineage over time . Vascularized allogenic bone transplantation can offer the unique benefits of vascularized autograft bone transplant with strong osteoinductive and osteoconductive properties while providing good size and shape matching and no donor site morbidity …”

Section: Discussionmentioning

confidence: 99%

Vascularized bone transplant chimerism mediated by vascular endothelial growth factor

et al. 2014

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Background Vascular Endothelial Growth Factor (VEGF) induces angiogenesis and osteogenesis in bone allotrasnplants. We aim to determine whether bone remodeling in VEGF-treated bone allotransplants results from repopulation with circulation-derived autogenous cells, or survival of allogenic transplant-derived cells. Methods Vascularized femoral bone grafts were transplanted from female Dark Agouti rats (DA;RT1a) to male Piebald Viral Glaxo (PVG;RT1c). Arteriovenous bundle implantation and short term immunosuppression were used to maintain cellular viability. VEGF was encapsulated in biodegradable microspheres and delivered intramedullary in the experimental group (n=22). In the control group (n=22), no VEGF was delivered. Rats were sacrificed at 4 or 18 weeks. Laser capture microdissection of bone remodeling areas was performed at the inner and outer cortex. Sex-mismatched genes were quantified with reverse transcription-polymerase chain reaction to determine the amount of male cells to total cells, defined as the relative Expression Ratio (rER). Results At 4 weeks rER was significantly higher at the inner cortex in VEGF treated transplants as compared to untreated transplants (0.622±0.225 vs 0.362±0.081, p=0.043). At 4 weeks, the outer cortex in the control group had a significantly higher rER (p=0.038), while in the VEGF group, the inner cortex had a higher rER (p=0.015). Over time, in the outer cortex the rER significantly increased to 0.634±0.106 at 18 weeks in VEGF treated rats (p=0.049). At 18 weeks, the rER was > 0.5 at all cortical areas in both groups. Conclusion These in vivo findings suggest a chemotactic effect of intramedullary applied VEGF on recipient derived bone and could imply that more rapid angiogenesis of vascularized allotransplants can be established with microencapsulated VEGF.

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Section: Discussionmentioning

confidence: 99%

Vascularized bone transplant chimerism mediated by vascular endothelial growth factor

et al. 2014

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“…In cases of intractable nonunion, pedicled bone grafts are desirable (Yoshida, 2009). The procedure can achieve quick restoration of the graft blood supply, which facilitates osseointegration at the nonunion of the fracture and improves the functional recovery of the reconstructive extremity (Cutting & McCarthy, 1983;Henry, 2007;Kakar, Duymaz, Steinmann, Shin, & Moran, 2011;Korompilias, Paschos, Lykissas, Kostas-Agnantis, Vekris, & Beris, 2011;Malizos, Dailiana, Innocenti, Mathoulin, Mattar, & Sauerbier, 2010;Shaffer, Davy, Field, Bensusan, & Kellis, 1988;Willems, Larsen, Friedrich, & Bishop, 2014).…”

Section: Introductionmentioning

confidence: 99%