Cell-lineage specificity of primary cilia during postnatal epididymal development

Bernet, Agathe; Bastien, Alexandre; Soulet, Denis; Jerczynski, Olivia; Roy, Christian; Alves, Maíra Bianchi Rodrigues; Lecours, Cynthia; Tremblay, Marie‐Ève; Bailey, Janice L.; Robert, Claude; Belleannée, Clémence

doi:10.1093/humrep/dey276

Cited by 11 publications

(20 citation statements)

References 33 publications

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“…Arl mice have much longer astrocytic and neuronal primary cilia than wild type FVB/N and C57BL/6 mice Double transgenic Arl mice have become a useful mouse model to study primary cilia and centrosomes [33]. While this strain has been commonly used in developmental studies [33][34][35], little is known about their morphological features and cilia expression pattern in the mature mouse brain. To characterize cilia morphology of Arl mice, we first measured cilia length in the hippocampus.…”

Section: Resultsmentioning

confidence: 99%

“…Arl mice contain fluorescent proteins that label Arl13bpositive primary cilia and Centrin2-positive centrosomes [33]. This strain has been used to investigate the roles of primary cilia in development [33][34][35]. We first found that overexpression of Arl13b significantly elongates both astrocyte and neuronal primary cilia in the hippocampus.…”

Section: Discussionmentioning

confidence: 99%

“…The Arl mice were introduced from the Jackson Laboratory (Stock No: 027967) and bred in house. The expression of Arl13b-mCherry and Centrin2-GFP was driven by CAG promoters [33][34][35]. This mouse strain has C57BL/6, FVB/N, and BALB/c mixed genetic background.…”

Section: Micementioning

confidence: 99%

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Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

et al. 2020

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Primary cilia are centriole-derived sensory organelles that are present in most mammalian cells, including astrocytes and neurons. Evidence is emerging that astrocyte and neuronal primary cilia demonstrate a dichotomy in the mature mouse brain. However, it is unknown how astrocytic and neuronal primary cilia change their morphology and ciliary proteins when exposed to reactive insults including epilepsy and traumatic brain injury. We used a double transgenic mouse strain (Arl13b-mCherry; Centrin2-GFP), in which we found spontaneous seizures, and a cortical injury model to examine the morphological changes of astrocytic and neuronal primary cilia under reactive conditions. Transgenic overexpression of Arl13b drastically increases the length of astrocytic and neuronal primary cilia in the hippocampus, as well as the cilia lengths of cultured astrocytes and neurons. Spontaneous seizures shorten Arl13b-positive astrocytic cilia and AC3-positive neuronal cilia in the hippocampus. In a cortical injury model, Arl13b is not detectable in primary cilia, but Arl13b protein relocates to the cell body and has robust expression in the proximity of injured tissues. In contrast, the number of AC3-positive cilia near injured tissues remains unchanged, but their lengths become shorter. These results on astrocytic cilia implicate Arl13b in regulating astrocyte proliferation and tissue regeneration, while the shortening of AC3-positive cilia suggests adaptive changes of neuronal primary cilia under excitotoxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

et al. 2020

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“…In the rete testis, the apical surface of the cuboidal epithelial cells bears a single long cilium—presumably a PC despite the lack of ultrastructural evidence—extending from a centriole pair (Fawcett & Dym, ; Dym, ). In the efferent ductules, the presence of multiple motile cilia and primary cilia‐like extensions has been reported to protrude from ciliated and non‐ciliated cells, respectively (Aire, ; Hess, ; Bernet et al ., ). While multiple motile cilia from the efferent ductules have recently been proposed to generate luminal flow turbulence to maintain sperm cells in suspension (Yuan et al ., ), the role of primary cilia in both the rete testis and the efferent ductules remains unknown.…”

Section: Diversity Of Cilia Found In the Male Reproductive Tractmentioning

confidence: 97%

“…Cilia are found throughout the male reproductive system as motile sperm flagella, multiple motile cilia (efferent ductules) (Ford et al ., ; Hess, ), and non‐motile sensory primary cilia (testis, rete testis, epididymis, and prostate) (Dym, ; Zhang et al ., ; Ou et al ., ; Dores et al ., ; Bernet et al ., ) (Table ). The broad diversity of ciliary extensions found at different stages of development confers to the male reproductive system a unique way to sense and control sperm function and spermatozoa surrounding environment.…”

Section: Diversity Of Cilia Found In the Male Reproductive Tractmentioning

confidence: 99%

Primary cilia: biosensors of the male reproductive tract

et al. 2019

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Background: The primary cilium is a microtubule-based organelle that extends transiently from the apical cell surface to act as a sensory antenna. Initially viewed as a cellular appendage of obscure significance, the primary cilium is now acknowledged as a key coordinator of signaling pathways during development and in tissue homeostasis. Objectives: The aim of this review was to present the structure and function of this overlooked organelle,with an emphasis on its epididymal context and contribution to male infertility issues. Materials and Methods: A systematic review has been performed in order to include main references relevant to the aforementioned topic. Results: Increasing evidence demonstrates that primary cilia dysfunctions are associated with impaired male reproductive system development and male infertility issues. Discussion: While a large amount of data exists regarding the role of primary cilia in most organs and tissues, few studies investigated the contribution of these organelles to male reproductive tract development and homeostasis. Conclusion: Functional studies of primary cilia constitute an emergent and exciting new area in reproductive biology research.

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Shear stress effects on epididymal epithelial cell via primary cilia mechanosensory signaling

Fakhari,

Campolina‐Silva,

Asayesh

et al. 2024

Journal Cellular Physiology

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Shear stress, resulting from fluid flow, is a fundamental mechanical stimulus affecting various cellular functions. The epididymis, essential for sperm maturation, offers a compelling model to study the effects of shear stress on cellular behavior. This organ undergoes extensive proliferation and differentiation until puberty, achieving full functionality as spermatozoa commence their post‐testicular maturation. Although the mechanical tension exerted by testicular fluid is hypothesized to drive epithelial proliferation and differentiation, the precise mechanisms remain unclear. Here we assessed whether the responsiveness of the epididymal cells to shear stress depends on functional primary cilia by combining microfluidic strategies on immortalized epididymal cells, calcium signaling assays, and high‐throughput gene expression analysis. We identified 97 genes overexpressed in response to shear stress, including early growth response (Egr) 2/3, cellular communication network factor (Ccn) 1/2, and Fos proto‐oncogene (Fos). While shear stress triggered a rapid increase of intracellular Ca2+, this response was abrogated following the impairment of primary ciliogenesis through pharmacological and siRNA approaches. Overall, our findings provide valuable insights into how mechanical forces influence the development of the male reproductive system, a requisite to sperm maturation.

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Cell-lineage specificity of primary cilia during postnatal epididymal development

Abstract: Study funded by an NSERC operating grant to CB (RGPIN-2015-109194). No competing interest to declare.

Cited by 11 publications

References 33 publications

Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

Primary cilia: biosensors of the male reproductive tract

Shear stress effects on epididymal epithelial cell via primary cilia mechanosensory signaling

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